FastQCFastQC Report
Thu 26 May 2016
SRR1547890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547890_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2100640
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127670.6077671566760606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31600.1504303450377028No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG253.891912E-545.0000041
CCCGATA207.0346205E-445.00000431
CGTTTTT72850.041.5717241
TATAACG451.929584E-840.0000041
GACCGAT10200.039.2647069
TCGATCA1100.038.86363617
ACACGCG2800.038.5714336
CCGCTAT356.250759E-638.5714331
CGGTCTA2000.038.25000431
GGCGATA6300.037.857148
GGACCGA11250.037.88
TAGGGCG6850.037.773725
AACGCTA301.1402434E-437.50000430
GGGCGAT28850.037.435017
TAGGGAC17950.037.1030655
TCCGCGA850.037.05882623
ATTGGGT7650.036.7647064
ATAGCGG4900.036.7346952
CGTAAGG3500.036.642862
TATAGCG1850.036.486491
Produced by FastQC (version 0.11.5)