##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547890_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2100640
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.527464010968085	31.0	31.0	33.0	30.0	34.0
2	31.964231853149517	31.0	31.0	34.0	30.0	34.0
3	32.02553650316094	33.0	31.0	34.0	30.0	34.0
4	35.80453718866631	37.0	35.0	37.0	35.0	37.0
5	35.74566655876304	37.0	35.0	37.0	35.0	37.0
6	35.77857938533018	37.0	35.0	37.0	35.0	37.0
7	36.04619592124305	37.0	35.0	37.0	35.0	37.0
8	36.06628313275954	37.0	35.0	37.0	35.0	37.0
9	37.82760396831442	39.0	38.0	39.0	35.0	39.0
10	37.30820083403153	39.0	37.0	39.0	34.0	39.0
11	37.1356339020489	39.0	37.0	39.0	34.0	39.0
12	36.627480672556935	39.0	35.0	39.0	33.0	39.0
13	36.42689275649326	39.0	35.0	39.0	32.0	39.0
14	37.43860870972656	40.0	35.0	41.0	32.0	41.0
15	37.606674632492954	40.0	35.0	41.0	33.0	41.0
16	37.7203623657552	40.0	35.0	41.0	33.0	41.0
17	37.66237956051489	40.0	35.0	41.0	33.0	41.0
18	37.53718390585726	39.0	36.0	41.0	33.0	41.0
19	37.46359395231929	39.0	36.0	41.0	33.0	41.0
20	37.32454870896489	39.0	35.0	41.0	32.0	41.0
21	37.20305430725874	39.0	35.0	41.0	32.0	41.0
22	37.129992764109986	39.0	35.0	41.0	32.0	41.0
23	37.04110794805393	39.0	35.0	41.0	32.0	41.0
24	37.0788978596999	39.0	35.0	41.0	32.0	41.0
25	37.045362365755196	39.0	35.0	41.0	32.0	41.0
26	36.95268584812248	39.0	35.0	41.0	32.0	41.0
27	36.88820787950339	39.0	35.0	41.0	32.0	41.0
28	36.81090239165207	39.0	35.0	41.0	31.0	41.0
29	36.81815256302841	38.0	35.0	41.0	31.0	41.0
30	36.742356615126816	38.0	35.0	41.0	31.0	41.0
31	36.5875885444436	38.0	35.0	41.0	31.0	41.0
32	36.438537778962605	38.0	35.0	41.0	31.0	41.0
33	36.315513843400105	38.0	35.0	41.0	30.0	41.0
34	36.14475921623886	38.0	35.0	41.0	30.0	41.0
35	36.03021126894661	38.0	35.0	41.0	30.0	41.0
36	35.904397707365376	38.0	35.0	41.0	29.0	41.0
37	35.801080623048215	38.0	35.0	41.0	29.0	41.0
38	35.67813666311219	38.0	35.0	40.0	28.0	41.0
39	35.60752484956966	38.0	35.0	40.0	28.0	41.0
40	35.43076205346942	38.0	35.0	40.0	27.0	41.0
41	35.348009654200624	38.0	35.0	40.0	26.0	41.0
42	35.28174080280296	38.0	34.0	40.0	26.0	41.0
43	35.22189189961155	38.0	34.0	40.0	26.0	41.0
44	35.07075081879808	38.0	34.0	40.0	26.0	41.0
45	34.94010254017823	38.0	34.0	40.0	25.0	41.0
46	34.886837820854595	37.0	34.0	40.0	25.0	41.0
47	34.80190560971894	37.0	34.0	40.0	24.0	41.0
48	34.70174851473836	37.0	34.0	40.0	24.0	41.0
49	34.574092657475816	37.0	34.0	40.0	24.0	41.0
50	34.49237327671567	37.0	34.0	40.0	24.0	41.0
51	34.181188590143954	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	2.0
11	5.0
12	16.0
13	24.0
14	37.0
15	61.0
16	137.0
17	357.0
18	752.0
19	1445.0
20	2574.0
21	4210.0
22	6458.0
23	9790.0
24	14934.0
25	22095.0
26	29447.0
27	33817.0
28	36218.0
29	39717.0
30	46058.0
31	55554.0
32	68941.0
33	91563.0
34	160321.0
35	238138.0
36	141746.0
37	188371.0
38	314303.0
39	593477.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.681201919415038	25.984461878284716	30.394070378551298	16.940265823748955
2	29.551565237261023	27.498762281971207	29.130169852997184	13.819502627770582
3	28.088582527229793	25.273964125218978	32.141966257902354	14.49548708964887
4	25.534122933962983	27.876171071673394	31.160646279229187	15.429059715134436
5	23.853063828166654	30.91529248229111	30.575158047071373	14.656485642470866
6	22.257169243659074	39.06595132911874	27.744068474369715	10.932810952852464
7	87.95571825729301	3.25962563790083	7.049280219361719	1.7353758854444359
8	88.53173318607662	2.6817065275344656	6.811257521517251	1.9753027648716583
9	83.57053088582526	4.462687561885901	8.939894508340315	3.026887043948511
10	39.39875466524488	24.51252951481453	20.213934800822607	15.874781019117984
11	34.62206756036256	21.184210526315788	25.347227511615504	18.846494401706146
12	33.293044024678196	21.62079175870211	27.106881712240078	17.97928250437962
13	25.094923451900375	29.502056516109377	28.106005788712014	17.297014243278237
14	19.293024982862367	32.68727625866403	30.228168558153705	17.7915302003199
15	17.925917815522887	25.653467514662196	39.884130550689314	16.5364841191256
16	21.45512796100236	23.53235204509102	37.26207251123467	17.750447482671948
17	21.796928555107016	22.519422652144108	29.0294386472694	26.654210145479475
18	23.393584812247695	24.49677241221723	32.358852540178226	19.750790235356845
19	25.935429202528752	26.58213687257217	27.846513443521975	19.635920481377102
20	26.89532713839592	26.21943788559677	27.577595399497294	19.307639576510017
21	23.63465420062457	25.999076471932366	31.117421357300636	19.24884797014243
22	22.94505484042958	23.46132607205423	30.301431944550234	23.292187142965954
23	20.210174042196662	27.242078604615738	29.878465610480614	22.669281742706985
24	20.847075177088886	25.881445654657625	33.8810076928936	19.39047147535989
25	20.62238175032371	26.87504760453957	31.05153667453728	21.451033970599436
26	19.15211554573844	30.33418386777363	28.978596999009827	21.5351035874781
27	19.11960164521289	29.60202604920405	31.725331327595402	19.55304097798766
28	16.99382093076396	28.356167644146545	34.800536979206335	19.849474445883157
29	18.403343742859317	26.384768451519534	34.55532599588697	20.656561809734175
30	19.991288369258893	27.380179373905094	32.74273554726179	19.885796709574226
31	24.287931297128495	26.82849036484119	28.313133140376266	20.570445197654045
32	24.193293472465534	27.83942036712621	28.48498552821997	19.482300632188284
33	23.394060857643385	27.772678802650617	27.85251161550765	20.98074872419834
34	19.87061086145175	27.61896374438266	30.50722636910656	22.003199025059033
35	19.719276030162234	27.541844390281057	31.02378322796862	21.715096351588087
36	24.176060629141595	26.120420443293472	29.2674137405743	20.43610518699063
37	20.915625714068092	29.321492497524567	29.752170767004344	20.010711021403
38	20.763624419224616	29.115793282047374	27.203423718485798	22.917158580242212
39	20.3613184553279	28.370401401477647	28.80722065656181	22.461059486632646
40	22.670091019879656	26.605891537817044	28.859776068245868	21.864241374057432
41	19.36809734176251	25.494230329804253	30.180040368649554	24.957631959783686
42	22.09436171833346	27.26583326986061	28.130855358366976	22.508949653438954
43	21.451510015995126	27.43459136263234	28.55386929697616	22.560029324396375
44	20.359081041968164	29.303831213344505	28.46303983547871	21.87404790920862
45	19.440218219209385	30.48132949958108	26.97635006474217	23.10210221646736
46	22.01990821844771	29.038435905247923	28.056497067560365	20.885158808744002
47	21.07105453576053	28.225017137634246	29.209240993221115	21.49468733338411
48	21.60141671109757	26.68672404600503	29.647678802650624	22.06418044024678
49	21.147269403610327	25.36127085078833	30.5850597913017	22.90639995429964
50	19.341295985985223	28.68778086678346	29.7314151877523	22.239507959479017
51	19.084945540406732	29.99352578261863	27.661379389138546	23.26014928783609
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1403.0
1	2599.5
2	3796.0
3	12991.5
4	22187.0
5	16540.0
6	10893.0
7	10149.0
8	9405.0
9	9781.0
10	10157.0
11	10131.0
12	10105.0
13	10008.5
14	9912.0
15	9733.5
16	9555.0
17	9176.0
18	8797.0
19	8735.5
20	8674.0
21	8735.5
22	8797.0
23	10200.0
24	11603.0
25	12223.0
26	16978.0
27	21113.0
28	24073.0
29	27033.0
30	32518.5
31	38004.0
32	43299.0
33	48594.0
34	54490.0
35	60386.0
36	66063.0
37	71740.0
38	80354.5
39	88969.0
40	109145.5
41	129322.0
42	146011.0
43	162700.0
44	169825.0
45	176950.0
46	180279.5
47	183609.0
48	184671.0
49	185733.0
50	175963.5
51	166194.0
52	154825.5
53	143457.0
54	128680.5
55	113904.0
56	105194.5
57	96485.0
58	87685.5
59	78886.0
60	69193.5
61	59501.0
62	51103.5
63	42706.0
64	35604.5
65	28503.0
66	22623.0
67	16743.0
68	13168.0
69	9593.0
70	7468.5
71	5344.0
72	4374.0
73	3404.0
74	2648.0
75	1296.0
76	700.0
77	525.5
78	351.0
79	326.5
80	302.0
81	226.0
82	150.0
83	136.5
84	123.0
85	87.5
86	52.0
87	40.0
88	28.0
89	21.5
90	15.0
91	15.5
92	16.0
93	12.0
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2100640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.466624379815492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4683342035653	15.855152974743842
2	7.840470458993888	3.20935927690535
3	2.778041525959386	1.7057139727002062
4	1.4146078839235117	1.158089928199526
5	0.8938903142129735	0.9147458648876087
6	0.631505155033071	0.7754867281187408
7	0.4750642543959383	0.6806073155699146
8	0.3769980571420806	0.6172702101957744
9	0.3103458786244261	0.5716559273066945
>10	4.819088519892287	25.680548680168574
>50	2.5898559994799357	37.15736686251695
>100	0.3977821336983227	10.372899836148248
>500	0.0033069771237403353	0.46420634864993915
>1k	4.724253033914765E-4	0.21967857199180618
>5k	0.0	0.0
>10k+	2.3621265169573825E-4	0.6172175018968727
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12767	0.6077671566760606	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3160	0.1504303450377028	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.520907913778658E-5	0.0	0.0	0.017280447863508266	0.0
2	9.520907913778658E-5	0.0	0.0	0.0978749333536446	0.0
3	9.520907913778658E-5	0.0	0.0	0.19203671262091554	0.0
4	9.520907913778658E-5	0.0	0.0	0.40382930916292176	0.0
5	9.520907913778658E-5	0.0	0.0	0.7846656257140681	0.0
6	9.520907913778658E-5	0.0	0.0	1.2587116307411075	0.0
7	9.520907913778658E-5	0.0	0.0	1.5219647345570875	0.0
8	9.520907913778658E-5	0.0	0.0	2.1063104577652525	0.0
9	9.520907913778658E-5	0.0	0.0	2.34471399192627	0.0
10	9.520907913778658E-5	0.0	0.0	2.771345875542692	0.0
11	9.520907913778658E-5	0.0	0.0	3.320749866707289	0.0
12	9.520907913778658E-5	0.0	0.0	3.7425260872876835	0.0
13	9.520907913778658E-5	0.0	0.0	3.9076662350521745	0.0
14	9.520907913778658E-5	0.0	0.0	3.981072435067408	0.0
15	9.520907913778658E-5	0.0	0.0	4.082946149744839	0.0
16	9.520907913778658E-5	0.0	0.0	4.315827557315866	0.0
17	9.520907913778658E-5	0.0	0.0	4.58017556554193	0.0
18	9.520907913778658E-5	0.0	0.0	4.889795490898012	0.0
19	9.520907913778658E-5	0.0	0.0	5.058125142813619	0.0
20	9.520907913778658E-5	0.0	0.0	5.244925356081956	0.0
21	9.520907913778658E-5	0.0	0.0	5.490755198415721	0.0
22	9.520907913778658E-5	0.0	0.0	5.741631121943788	0.0
23	9.520907913778658E-5	0.0	0.0	5.999504912788484	0.0
24	9.520907913778658E-5	0.0	0.0	6.192922157056897	0.0
25	9.520907913778658E-5	0.0	0.0	6.357395841267423	0.0
26	9.520907913778658E-5	0.0	0.0	6.52529705232691	0.0
27	9.520907913778658E-5	0.0	0.0	6.712144870134816	0.0
28	9.520907913778658E-5	0.0	0.0	6.889900220885064	0.0
29	9.520907913778658E-5	0.0	0.0	7.090410541549242	0.0
30	9.520907913778658E-5	0.0	0.0	7.321673394774926	0.0
31	9.520907913778658E-5	0.0	0.0	7.538083631655114	0.0
32	9.520907913778658E-5	0.0	0.0	7.735214030009902	0.0
33	9.520907913778658E-5	0.0	0.0	7.933534541853911	0.0
34	9.520907913778658E-5	0.0	0.0	8.144613070302384	0.0
35	9.520907913778658E-5	0.0	0.0	8.395727016528296	0.0
36	9.520907913778658E-5	0.0	0.0	8.601949881940742	0.0
37	9.520907913778658E-5	0.0	0.0	8.826167263310229	0.0
38	9.520907913778658E-5	0.0	0.0	9.06704623352883	0.0
39	9.520907913778658E-5	0.0	0.0	9.444788255007998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGCG	25	3.891912E-5	45.000004	1
CCCGATA	20	7.0346205E-4	45.000004	31
CGTTTTT	7285	0.0	41.571724	1
TATAACG	45	1.929584E-8	40.000004	1
GACCGAT	1020	0.0	39.264706	9
TCGATCA	110	0.0	38.863636	17
ACACGCG	280	0.0	38.57143	36
CCGCTAT	35	6.250759E-6	38.57143	31
CGGTCTA	200	0.0	38.250004	31
GGCGATA	630	0.0	37.85714	8
GGACCGA	1125	0.0	37.8	8
TAGGGCG	685	0.0	37.77372	5
AACGCTA	30	1.1402434E-4	37.500004	30
GGGCGAT	2885	0.0	37.43501	7
TAGGGAC	1795	0.0	37.103065	5
TCCGCGA	85	0.0	37.058826	23
ATTGGGT	765	0.0	36.764706	4
ATAGCGG	490	0.0	36.734695	2
CGTAAGG	350	0.0	36.64286	2
TATAGCG	185	0.0	36.48649	1
>>END_MODULE