FastQCFastQC Report
Thu 26 May 2016
SRR1547889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547889_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3439206
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197470.5741732248664372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG4050.043.3333362
CGTTTTT99300.042.3716051
CGACGGT2650.039.90566328
GCGCGAC3800.039.671059
TACGGGA13900.039.3345344
CGGTCTA2750.039.27272831
TATGGGA39100.038.95784
TACCGGG4050.038.888893
CGGGTAT1800.038.756
AGGGATT69850.038.2355046
TAAGGGA50250.037.970154
TACGCGG1900.037.8947372
TAGTACG950.037.8947371
TAGGGAT64600.037.546445
TAGGGAC43700.037.534325
TATAGCG1500.037.51
GGGCGAT48800.037.2079937
ATAGGGA54600.037.04674
TTAGGGA53750.036.920934
TCACGAC3050.036.88524625
Produced by FastQC (version 0.11.5)