##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547889_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3439206
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415167047277773	31.0	31.0	33.0	30.0	34.0
2	31.830300365840255	31.0	31.0	34.0	30.0	34.0
3	31.881051324055612	33.0	31.0	34.0	30.0	34.0
4	35.67139275751438	37.0	35.0	37.0	33.0	37.0
5	35.58873472539883	37.0	35.0	37.0	33.0	37.0
6	35.62682840167178	37.0	35.0	37.0	33.0	37.0
7	35.98852438615192	37.0	35.0	37.0	35.0	37.0
8	36.01794367653464	37.0	35.0	37.0	35.0	37.0
9	37.75700466910095	39.0	38.0	39.0	35.0	39.0
10	37.25224339571401	39.0	37.0	39.0	34.0	39.0
11	36.951827253150874	39.0	37.0	39.0	33.0	39.0
12	36.531069962078455	39.0	35.0	39.0	32.0	39.0
13	36.38264762273618	39.0	35.0	39.0	32.0	39.0
14	37.416081793297636	40.0	35.0	41.0	32.0	41.0
15	37.586931983719495	40.0	35.0	41.0	32.0	41.0
16	37.68111011669554	40.0	35.0	41.0	33.0	41.0
17	37.60382512707875	40.0	35.0	41.0	33.0	41.0
18	37.502564254656455	39.0	36.0	41.0	32.0	41.0
19	37.4438777438746	39.0	36.0	41.0	32.0	41.0
20	37.3061154231529	39.0	35.0	41.0	32.0	41.0
21	37.18383400122005	39.0	35.0	41.0	32.0	41.0
22	37.09661997565717	39.0	35.0	41.0	32.0	41.0
23	37.00870229930978	39.0	35.0	41.0	32.0	41.0
24	37.02887701405499	39.0	35.0	41.0	32.0	41.0
25	37.00351912621692	39.0	35.0	41.0	32.0	41.0
26	36.90488327829156	39.0	35.0	41.0	31.0	41.0
27	36.81856393597825	39.0	35.0	41.0	31.0	41.0
28	36.763117126453025	39.0	35.0	41.0	31.0	41.0
29	36.79179729274722	39.0	35.0	41.0	31.0	41.0
30	36.69843940723528	38.0	35.0	41.0	31.0	41.0
31	36.57137955679305	38.0	35.0	41.0	31.0	41.0
32	36.430382768580884	38.0	35.0	41.0	30.0	41.0
33	36.33974498765122	38.0	35.0	41.0	30.0	41.0
34	36.209682990783335	38.0	35.0	41.0	30.0	41.0
35	36.102851355807125	38.0	35.0	41.0	30.0	41.0
36	35.992100211502304	38.0	35.0	41.0	29.0	41.0
37	35.91096055310441	38.0	35.0	40.0	29.0	41.0
38	35.76390509902576	38.0	35.0	40.0	29.0	41.0
39	35.67907679854013	38.0	35.0	40.0	28.0	41.0
40	35.53921980829296	38.0	35.0	40.0	27.0	41.0
41	35.46393062817406	38.0	35.0	40.0	27.0	41.0
42	35.40378302433759	38.0	34.0	40.0	27.0	41.0
43	35.34981766140208	38.0	34.0	40.0	27.0	41.0
44	35.176099657886155	38.0	34.0	40.0	26.0	41.0
45	35.038076230385734	38.0	34.0	40.0	26.0	41.0
46	34.98812865527683	38.0	34.0	40.0	26.0	41.0
47	34.88915930014079	37.0	34.0	40.0	25.0	41.0
48	34.77557726986985	37.0	34.0	40.0	24.0	41.0
49	34.625908712650535	37.0	34.0	40.0	24.0	41.0
50	34.54630661844624	37.0	34.0	40.0	24.0	41.0
51	34.2295681038007	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	5.0
12	9.0
13	15.0
14	27.0
15	87.0
16	230.0
17	511.0
18	1108.0
19	2211.0
20	4030.0
21	6562.0
22	10338.0
23	15541.0
24	23503.0
25	34555.0
26	46269.0
27	54579.0
28	60648.0
29	68736.0
30	80351.0
31	96755.0
32	119496.0
33	154365.0
34	250913.0
35	363202.0
36	246567.0
37	325181.0
38	522760.0
39	950541.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.31708132632939	24.254493624400517	29.91324160285833	13.515183446411758
2	30.330605378101804	26.03362520302651	30.267015119187395	13.368754299684287
3	28.95691621845275	25.227218142792264	32.296029955751415	13.519835683003578
4	25.15342203985455	28.259400570945736	31.323043749051383	15.264133640148337
5	23.44799933473017	31.84298934114444	29.87736122814394	14.831650095981455
6	21.069805065471506	40.948666639916304	26.743876348203628	11.237651946408562
7	88.49548413209328	3.180385240081577	6.564160448661697	1.7599701791634466
8	89.11812784695073	2.2500251511540745	6.181194147718979	2.4506528541762256
9	84.29154868885435	4.699602175618442	8.1488576142284	2.859991521298811
10	41.63795364395154	36.449779396756114	12.509515277654204	9.402751681638145
11	30.01588157266532	24.11867739239813	28.712848256254496	17.152592778682056
12	29.528094566013202	22.54534913000268	29.646435834317575	18.280120469666546
13	23.309246378379196	29.643208345182	29.673476959507518	17.37406831693129
14	17.370317451179137	33.01055534329726	30.632128462209007	18.98699874331459
15	16.3130966856885	27.064502678815984	39.296744655597834	17.325655979897686
16	20.127378237883978	25.252660061653764	36.46882449030387	18.151137210158392
17	19.967951905178115	25.202386829983432	29.452088650694375	25.37757261414408
18	21.82608427642892	25.47262362301066	32.66044546328426	20.04084663727616
19	24.459977099365375	27.43534408814127	27.979539463469184	20.125139349024167
20	25.565842813719215	27.265682834933415	27.944676765509247	19.223797585838128
21	23.3245406061748	27.109629373756615	30.821387262059908	18.74444275800868
22	22.58120042823838	23.362747099185103	30.4226615096624	23.633390962914113
23	19.424396212381577	28.34186146453571	29.39030113345929	22.84344118962342
24	19.741649671464867	26.52347663966625	33.84211355760603	19.892760131262854
25	19.940038485627205	27.5804357168486	30.929987910000158	21.54953788752404
26	19.315853717398728	30.90472045001085	27.90335908927816	21.876066743312265
27	18.85060098173823	29.43504983417684	30.626923772521913	21.087425411563018
28	17.58144176301158	28.386028635679285	33.61630562403066	20.416223977278477
29	18.21687912849652	26.100762792342184	32.88465419053119	22.797703888630107
30	20.030757099167655	27.13998521751823	31.30045132510236	21.52880635821175
31	23.496702436550763	26.70471033139626	27.532633985867665	22.265953246185312
32	23.72884904248248	27.09453868131191	27.848113779750328	21.328498496455286
33	22.249583188677853	27.479133265061762	27.949706996324153	22.321576549936236
34	19.080363316416637	27.326481751892732	30.247155884236072	23.34599904745456
35	19.460625504840362	27.596573162526468	30.33618806201199	22.606613270621185
36	23.23399645150654	26.026705001096182	29.973953290381562	20.765345257015717
37	20.552301897589153	28.32900966095081	30.2258428253498	20.892845616110232
38	19.643022255718325	29.194965349560338	29.10581686586962	22.056195528851717
39	19.747319584811144	27.58450642386644	30.296120674364957	22.37205331695746
40	22.562475175956312	25.38489988677619	30.318684021835274	21.733940915432225
41	19.07748474502545	24.897345491953665	30.608634667420333	25.41653509560056
42	21.17398608864953	25.885102549832723	29.32394860906849	23.616962752449258
43	21.1134779364772	26.217446701360718	29.50619997755296	23.162875384609123
44	20.351790500481798	28.50358483905878	28.76335409975442	22.381270560704998
45	19.494586831960632	29.297663472324714	27.638966668469404	23.568783027245242
46	21.90432908060756	27.997886721528165	28.106807210734107	21.99097698713017
47	20.559949011486953	26.7700742555113	29.91030487850975	22.759671854491998
48	21.108912929321477	25.5866615724676	30.355843761612416	22.948581736598506
49	21.13598313099012	24.988354870281103	30.339415551147564	23.536246447581217
50	19.38211901235343	27.430197551411577	30.715985026776526	22.47169840945846
51	19.03099145558597	27.984656923720188	28.605497896898296	24.37885372379555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1516.0
1	2518.0
2	3520.0
3	17774.0
4	32028.0
5	23397.5
6	14767.0
7	13546.0
8	12325.0
9	13023.0
10	13721.0
11	13782.5
12	13844.0
13	13673.0
14	13502.0
15	12998.0
16	12494.0
17	12006.5
18	11519.0
19	11768.0
20	12017.0
21	14248.5
22	16480.0
23	18353.0
24	20226.0
25	22367.5
26	31817.5
27	39126.0
28	48156.0
29	57186.0
30	63150.5
31	69115.0
32	81283.5
33	93452.0
34	104434.5
35	115417.0
36	122893.5
37	130370.0
38	143890.5
39	157411.0
40	182025.5
41	206640.0
42	235513.5
43	264387.0
44	274765.0
45	285143.0
46	299475.5
47	313808.0
48	313950.0
49	314092.0
50	298303.5
51	282515.0
52	252778.5
53	223042.0
54	199178.0
55	175314.0
56	160207.0
57	145100.0
58	127857.0
59	110614.0
60	98077.5
61	85541.0
62	74326.5
63	63112.0
64	52652.5
65	42193.0
66	33475.5
67	24758.0
68	20198.0
69	15638.0
70	12862.0
71	10086.0
72	7747.0
73	5408.0
74	4201.0
75	2546.0
76	2098.0
77	1569.5
78	1041.0
79	831.5
80	622.0
81	456.0
82	290.0
83	209.5
84	129.0
85	94.0
86	59.0
87	38.5
88	18.0
89	13.5
90	9.0
91	6.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3439206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.470507514415797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9475779559147	15.546854652482315
2	9.354024551038117	3.8296325972411123
3	3.326398693198044	2.0427920833516033
4	1.7202095036213811	1.4085424628100378
5	1.0012490857252747	1.0248038466570593
6	0.6824577963670354	0.8382154469281828
7	0.48168325161325526	0.6902210435202165
8	0.38253876996256175	0.6264610212059197
9	0.3218335194295645	0.5929285930086404
>10	3.849757360598357	19.680338899064378
>50	2.2062902347438413	32.99774636012886
>100	0.720037428868368	18.861907242530044
>500	0.0034781554650622744	0.509166193734105
>1k	0.0023187703100415163	0.7645651194380559
>5k	0.0	0.0
>10k+	1.4492314437759477E-4	0.5858244378993982
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19747	0.5741732248664372	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09086399593394522	0.0
2	0.0	0.0	0.0	0.31748607091287934	0.0
3	0.0	0.0	0.0	0.5054073527436275	0.0
4	0.0	0.0	0.0	0.7977713460606896	0.0
5	0.0	0.0	0.0	1.4222759555548576	0.0
6	0.0	0.0	0.0	2.250519451291955	0.0
7	0.0	0.0	0.0	2.725047583657391	0.0
8	0.0	0.0	0.0	3.800528377770916	0.0
9	0.0	0.0	0.0	4.2464452550966705	0.0
10	0.0	0.0	0.0	4.925177497364217	0.0
11	0.0	0.0	0.0	5.642232538556865	0.0
12	0.0	0.0	0.0	6.19625576368499	0.0
13	0.0	0.0	0.0	6.4348573478878555	0.0
14	0.0	0.0	0.0	6.540724806830414	0.0
15	0.0	0.0	0.0	6.672295872942766	0.0
16	0.0	0.0	0.0	6.951459144930545	0.0
17	0.0	0.0	0.0	7.30968136250053	0.0
18	0.0	0.0	0.0	7.747951125928485	0.0
19	0.0	0.0	0.0	7.98757038688581	0.0
20	0.0	0.0	0.0	8.237744409610823	0.0
21	0.0	0.0	0.0	8.567471678055924	0.0
22	0.0	0.0	0.0	8.944448224386676	0.0
23	0.0	0.0	0.0	9.337823904703585	0.0
24	0.0	0.0	0.0	9.624663367067864	0.0
25	0.0	0.0	0.0	9.874401242612393	0.0
26	0.0	0.0	0.0	10.108408743180838	0.0
27	0.0	0.0	0.0	10.36733478599421	0.0
28	0.0	0.0	0.0	10.62495238726613	0.0
29	2.907647869886247E-5	0.0	0.0	10.906936077687698	0.0
30	2.907647869886247E-5	0.0	0.0	11.273648627037753	0.0
31	5.815295739772494E-5	0.0	0.0	11.565431090780837	0.0
32	5.815295739772494E-5	0.0	0.0	11.840203814485088	0.0
33	5.815295739772494E-5	0.0	0.0	12.11977415717465	0.0
34	5.815295739772494E-5	0.0	0.0	12.410422638248479	0.0
35	5.815295739772494E-5	0.0	0.0	12.745616284688966	0.0
36	5.815295739772494E-5	0.0	0.0	13.027774433982728	0.0
37	5.815295739772494E-5	0.0	0.0	13.320952568703358	0.0
38	8.72294360965874E-5	0.0	0.0	13.63657774497951	0.0
39	8.72294360965874E-5	0.0	0.0	14.069235748018583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	405	0.0	43.333336	2
CGTTTTT	9930	0.0	42.371605	1
CGACGGT	265	0.0	39.905663	28
GCGCGAC	380	0.0	39.67105	9
TACGGGA	1390	0.0	39.334534	4
CGGTCTA	275	0.0	39.272728	31
TATGGGA	3910	0.0	38.9578	4
TACCGGG	405	0.0	38.88889	3
CGGGTAT	180	0.0	38.75	6
AGGGATT	6985	0.0	38.235504	6
TAAGGGA	5025	0.0	37.97015	4
TACGCGG	190	0.0	37.894737	2
TAGTACG	95	0.0	37.894737	1
TAGGGAT	6460	0.0	37.54644	5
TAGGGAC	4370	0.0	37.53432	5
TATAGCG	150	0.0	37.5	1
GGGCGAT	4880	0.0	37.207993	7
ATAGGGA	5460	0.0	37.0467	4
TTAGGGA	5375	0.0	36.92093	4
TCACGAC	305	0.0	36.885246	25
>>END_MODULE