FastQCFastQC Report
Thu 26 May 2016
SRR1547888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547888_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3316231
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173980.524631728006885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93050.041.4454571
CACGACC3950.040.44303527
ACCCGTA403.4608456E-739.37533
GCGATAC1550.039.1935469
GGGCGAT48200.038.7448127
ATAGGGC14650.037.9351544
CTATGCG1250.037.81
ATAGGGA41650.037.6530574
AGGGCGA24200.037.0971076
TAGGGAC33350.037.0389825
ACGGGAT16000.036.843755
GTAGGGA43300.036.84184
TAGGGAT51550.036.838025
TAGGGTA11800.036.8008465
TCACGAC3550.036.76056325
TAAGGGA41600.036.7247624
GCGTAAG3250.036.6923071
TACGGGA12250.036.5510184
AGGGATT55400.036.4711236
ATAGCGG6300.036.4285742
Produced by FastQC (version 0.11.5)