##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547888_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3316231
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444028175359318	31.0	31.0	33.0	30.0	34.0
2	31.85695206395453	31.0	31.0	34.0	30.0	34.0
3	31.930268428224693	33.0	31.0	34.0	30.0	34.0
4	35.71860404175704	37.0	35.0	37.0	35.0	37.0
5	35.61731737023145	37.0	35.0	37.0	33.0	37.0
6	35.66880564110281	37.0	35.0	37.0	33.0	37.0
7	35.97358085127363	37.0	35.0	37.0	35.0	37.0
8	35.991267496142456	37.0	35.0	37.0	35.0	37.0
9	37.73569513100867	39.0	38.0	39.0	35.0	39.0
10	37.26198747915932	39.0	37.0	39.0	34.0	39.0
11	37.00892579557938	39.0	37.0	39.0	33.0	39.0
12	36.5592381833473	39.0	35.0	39.0	32.0	39.0
13	36.41163356834913	39.0	35.0	39.0	32.0	39.0
14	37.446009340121364	40.0	36.0	41.0	32.0	41.0
15	37.61508320741227	40.0	36.0	41.0	33.0	41.0
16	37.69121300657282	40.0	35.0	41.0	33.0	41.0
17	37.61730380060979	40.0	35.0	41.0	33.0	41.0
18	37.51016409894245	39.0	36.0	41.0	32.0	41.0
19	37.43665956925196	39.0	36.0	41.0	32.0	41.0
20	37.27699548071289	39.0	35.0	41.0	32.0	41.0
21	37.160596472320535	39.0	35.0	41.0	32.0	41.0
22	37.08958634063791	39.0	35.0	41.0	32.0	41.0
23	36.99392231723303	39.0	35.0	41.0	32.0	41.0
24	37.026859407562384	39.0	35.0	41.0	32.0	41.0
25	37.000195402551874	39.0	35.0	41.0	32.0	41.0
26	36.89397541968578	39.0	35.0	41.0	31.0	41.0
27	36.81919986876668	39.0	35.0	41.0	31.0	41.0
28	36.74031272248526	39.0	35.0	41.0	31.0	41.0
29	36.74378262551674	39.0	35.0	41.0	31.0	41.0
30	36.646565634299904	38.0	35.0	41.0	31.0	41.0
31	36.50746947362835	38.0	35.0	41.0	30.0	41.0
32	36.39867035800582	38.0	35.0	41.0	30.0	41.0
33	36.301216049183544	38.0	35.0	41.0	30.0	41.0
34	36.155200286107934	38.0	35.0	41.0	30.0	41.0
35	36.07255676700447	38.0	35.0	41.0	30.0	41.0
36	35.971523998177446	38.0	35.0	41.0	29.0	41.0
37	35.875755337912224	38.0	35.0	41.0	29.0	41.0
38	35.75534243543348	38.0	35.0	40.0	29.0	41.0
39	35.66515058812248	38.0	35.0	40.0	28.0	41.0
40	35.518441568153726	38.0	35.0	40.0	27.0	41.0
41	35.444399078351296	38.0	34.0	40.0	27.0	41.0
42	35.37663540326352	38.0	34.0	40.0	27.0	41.0
43	35.31698394954996	38.0	34.0	40.0	27.0	41.0
44	35.09814455024394	38.0	34.0	40.0	26.0	41.0
45	34.94573779691463	38.0	34.0	40.0	25.0	41.0
46	34.930771710414625	37.0	34.0	40.0	25.0	41.0
47	34.859040881048394	37.0	34.0	40.0	25.0	41.0
48	34.772507705283495	37.0	34.0	40.0	25.0	41.0
49	34.628300622001305	37.0	34.0	40.0	24.0	41.0
50	34.55709659550254	37.0	34.0	40.0	24.0	41.0
51	34.224535323383684	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	9.0
12	9.0
13	19.0
14	39.0
15	90.0
16	245.0
17	584.0
18	1264.0
19	2530.0
20	4474.0
21	7031.0
22	10854.0
23	15805.0
24	23186.0
25	33253.0
26	44406.0
27	52022.0
28	57255.0
29	64922.0
30	76540.0
31	92541.0
32	114632.0
33	148783.0
34	246463.0
35	337331.0
36	242625.0
37	323109.0
38	511972.0
39	904107.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.65540579048926	24.118343987496647	28.999638444969605	13.22661177704448
2	31.19179574643624	25.723509610759926	29.386493281077225	13.69820136172661
3	29.85576095272012	25.39186805744232	30.888409160881736	13.863961828955823
4	25.925003415021454	27.797912750951305	30.641261118420278	15.635822715606965
5	23.848338671220432	31.998615295496602	29.313579180702433	14.839466852580536
6	21.99581392249213	41.03088717281758	26.107740986680362	10.865557918009934
7	88.32852717437356	3.5682978658603695	6.2201637943798245	1.8830111653862471
8	88.62684776784246	2.9948758093148515	6.063841752881509	2.31443466996117
9	83.60856044105492	5.5459646809887495	7.768970255690872	3.0765046222654573
10	41.307466216919146	33.08753220146606	13.705136946129507	11.899864635485285
11	32.39343097631016	23.95267398441182	26.467034413465168	17.18686062581286
12	30.577423587198844	22.00630776324086	28.596349289298605	18.819919360261693
13	24.230670300108766	27.67952534066535	29.01046398758108	19.079340371644797
14	18.60108659499293	30.88726328171952	30.5858367526267	19.92581337066085
15	18.125426123813448	26.205080406039265	37.43424387504972	18.235249595097567
16	22.139470983776462	24.739380338703786	34.0974437546721	19.023704922847653
17	22.20303712256474	24.254944845518903	27.982218367779566	25.559799664136783
18	23.78715475490097	24.869799480193024	30.994372828672066	20.348672936233935
19	26.292257686512187	26.491429577734483	27.082039821713266	20.134272914040068
20	26.919747146685502	26.549718641433607	27.26115882759675	19.269375384284146
21	25.247969758439627	26.056869982820857	30.04290111273913	18.652259146000382
22	24.35508865335376	23.059310403889235	29.72724155826298	22.858359384494022
23	21.53586405772095	26.98865066999253	29.013449304345805	22.462035967940714
24	21.396760358370692	26.137021214746497	32.77247574128582	19.693742685596995
25	21.74963083090412	26.75591658120318	29.504307751782065	21.990144836110634
26	20.06865625464571	29.521978414652057	27.54322602979105	22.866139300911183
27	20.05005079561707	27.878033828162152	30.45306554338344	21.618849832837338
28	18.885113853648917	27.0874676703764	32.88193735599239	21.145481119982293
29	19.642570134589537	25.940110927133848	32.24681875297589	22.170500185300725
30	21.174248717896912	26.582828518278735	30.859822491255883	21.383100272568466
31	25.096201078875385	25.957570507000266	27.629438359390523	21.316790054733822
32	25.33939885369867	26.111600790174144	27.45885313779408	21.0901472183331
33	23.93388759709441	26.529605446665204	27.26670729511907	22.269799661121315
34	20.722229543116867	26.54507481535514	29.557591132825184	23.175104508702802
35	20.446132974452023	26.84619979730001	29.575864889991077	23.131802338256897
36	24.531734972624044	25.991253323426506	28.26802475460847	21.208986949340982
37	21.00239699827907	28.53028030918232	28.7760412347632	21.691281457775407
38	21.636912506999664	28.433182127541777	27.668368096191127	22.261537269267432
39	21.23332180418071	26.440498264445388	28.94985301084273	23.376326920531167
40	22.664434413646095	25.892948953194157	28.670771125413157	22.771845507746598
41	20.27325599453114	25.01231066231514	29.82081163827248	24.893621704881234
42	21.968523905602474	25.974306373711602	28.01704706336802	24.0401226573179
43	21.814252384710233	26.078340139755042	28.23322621373481	23.87418126179992
44	21.3024967199209	27.435091222535462	28.362047155339905	22.900364902203737
45	20.384918903417766	28.425130818691457	26.886546805695982	24.30340347219479
46	22.258823344935863	27.158240786000732	27.866906738402726	22.71602913066068
47	21.029958407601885	26.809441199964656	29.26017518080013	22.900425211633326
48	21.9369519192119	25.95606277126051	29.4952914920583	22.611693817469288
49	22.144717904150827	24.970817774756945	29.616965766256936	23.267498554835296
50	20.622296818285577	27.41802365396138	29.087991759319543	22.871687768433503
51	20.20393030521698	27.414013076893617	28.19565343909999	24.186403178789416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1796.0
1	3119.0
2	4442.0
3	16991.0
4	29540.0
5	21840.5
6	14141.0
7	13209.5
8	12278.0
9	12750.0
10	13222.0
11	13336.0
12	13450.0
13	13358.5
14	13267.0
15	12718.5
16	12170.0
17	11785.0
18	11400.0
19	11048.5
20	10697.0
21	11720.5
22	12744.0
23	15374.0
24	18004.0
25	19710.0
26	27629.0
27	33842.0
28	39014.5
29	44187.0
30	52787.5
31	61388.0
32	68627.0
33	75866.0
34	87927.0
35	99988.0
36	107305.5
37	114623.0
38	127539.5
39	140456.0
40	161687.5
41	182919.0
42	205579.0
43	228239.0
44	239886.5
45	251534.0
46	257702.5
47	263871.0
48	267178.0
49	270485.0
50	262726.5
51	254968.0
52	236883.0
53	218798.0
54	201089.5
55	183381.0
56	172752.5
57	162124.0
58	153849.5
59	145575.0
60	133640.0
61	121705.0
62	105497.5
63	89290.0
64	77991.0
65	66692.0
66	56209.0
67	45726.0
68	37077.0
69	28428.0
70	22916.5
71	17405.0
72	14291.5
73	11178.0
74	9066.5
75	5683.0
76	4411.0
77	3066.5
78	1722.0
79	1351.5
80	981.0
81	694.5
82	408.0
83	348.5
84	289.0
85	192.0
86	95.0
87	71.0
88	47.0
89	48.0
90	49.0
91	30.0
92	11.0
93	12.0
94	13.0
95	12.5
96	12.0
97	7.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3316231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.681767594977792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57746211409618	15.268615799378354
2	8.261432862619005	3.2519920320715863
3	2.763782226989171	1.6318835842419306
4	1.3898928644705	1.0942219336170538
5	0.8555064036994966	0.8418939106814372
6	0.6217256813968085	0.7341996221448714
7	0.4570951398690148	0.6297508218187071
8	0.3748700379088096	0.5902483971553364
9	0.29110952671896034	0.5156595044609901
>10	4.202354992163544	21.3984070489342
>50	2.5425814874387074	36.238930312960846
>100	0.6546788830584366	15.945850188149116
>500	0.005318010529702201	0.7395706952509885
>1k	0.002033356967239077	0.583184617500613
>5k	0.0	0.0
>10k+	1.5641207440300592E-4	0.5355915316340617
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17398	0.524631728006885	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.04641443855992E-5	0.0	0.0	0.053223071613527524	0.0
2	9.04641443855992E-5	0.0	0.0	0.17589245140040002	0.0
3	9.04641443855992E-5	0.0	0.0	0.2707591841460984	0.0
4	9.04641443855992E-5	0.0	0.0	0.4439980206445208	0.0
5	9.04641443855992E-5	0.0	0.0	0.7874602221618457	0.0
6	9.04641443855992E-5	0.0	0.0	1.2548583014874417	0.0
7	9.04641443855992E-5	0.0	0.0	1.5176264862128122	0.0
8	9.04641443855992E-5	0.0	0.0	2.1407133580260242	0.0
9	9.04641443855992E-5	0.0	0.0	2.413703991066967	0.0
10	9.04641443855992E-5	0.0	0.0	2.8683164713193983	0.0
11	9.04641443855992E-5	0.0	0.0	3.3323070678731366	0.0
12	9.04641443855992E-5	0.0	0.0	3.697118807465463	0.0
13	9.04641443855992E-5	0.0	0.0	3.8567277128764554	0.0
14	9.04641443855992E-5	0.0	0.0	3.939411940844893	0.0
15	9.04641443855992E-5	0.0	0.0	4.042480756014886	0.0
16	9.04641443855992E-5	0.0	0.0	4.2513021559716435	0.0
17	9.04641443855992E-5	0.0	0.0	4.501797371775368	0.0
18	9.04641443855992E-5	0.0	0.0	4.812240160591949	0.0
19	9.04641443855992E-5	0.0	0.0	4.993349377651919	0.0
20	9.04641443855992E-5	0.0	0.0	5.183897020442786	0.0
21	9.04641443855992E-5	0.0	0.0	5.426672629258939	0.0
22	9.04641443855992E-5	0.0	0.0	5.695954232380072	0.0
23	9.04641443855992E-5	0.0	0.0	5.963155160180337	0.0
24	9.04641443855992E-5	0.0	0.0	6.17496187690182	0.0
25	9.04641443855992E-5	0.0	0.0	6.3590262560117194	0.0
26	9.04641443855992E-5	0.0	0.0	6.536004277144746	0.0
27	9.04641443855992E-5	0.0	0.0	6.73053234228858	0.0
28	9.04641443855992E-5	0.0	0.0	6.9266586073165595	0.0
29	9.04641443855992E-5	0.0	0.0	7.153783919154003	0.0
30	9.04641443855992E-5	0.0	0.0	7.427890276642369	0.0
31	9.04641443855992E-5	0.0	0.0	7.65968956927307	0.0
32	9.04641443855992E-5	0.0	0.0	7.877768466671954	0.0
33	9.04641443855992E-5	0.0	0.0	8.101124439159998	0.0
34	9.04641443855992E-5	0.0	0.0	8.335818584411038	0.0
35	9.04641443855992E-5	0.0	0.0	8.60323059521487	0.0
36	9.04641443855992E-5	0.0	0.0	8.831923952221663	0.0
37	9.04641443855992E-5	0.0	0.0	9.072015791421045	0.0
38	9.04641443855992E-5	0.0	0.0	9.357882487679538	0.0
39	9.04641443855992E-5	0.0	0.0	9.78101344568578	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9305	0.0	41.445457	1
CACGACC	395	0.0	40.443035	27
ACCCGTA	40	3.4608456E-7	39.375	33
GCGATAC	155	0.0	39.193546	9
GGGCGAT	4820	0.0	38.744812	7
ATAGGGC	1465	0.0	37.935154	4
CTATGCG	125	0.0	37.8	1
ATAGGGA	4165	0.0	37.653057	4
AGGGCGA	2420	0.0	37.097107	6
TAGGGAC	3335	0.0	37.038982	5
ACGGGAT	1600	0.0	36.84375	5
GTAGGGA	4330	0.0	36.8418	4
TAGGGAT	5155	0.0	36.83802	5
TAGGGTA	1180	0.0	36.800846	5
TCACGAC	355	0.0	36.760563	25
TAAGGGA	4160	0.0	36.724762	4
GCGTAAG	325	0.0	36.692307	1
TACGGGA	1225	0.0	36.551018	4
AGGGATT	5540	0.0	36.471123	6
ATAGCGG	630	0.0	36.428574	2
>>END_MODULE