FastQCFastQC Report
Thu 26 May 2016
SRR1547887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547887_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1876652
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550132.9314438691883207No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53920.2873201850955851No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT22280.11872206461293835No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20060.10689248725922547No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC207.0344284E-445.0000048
AATGCGT207.0344284E-445.00000417
CGTTTTT166950.043.9487841
TAGTGCG1450.043.4482731
CGACGGT2400.043.12499628
TAATACG650.041.5384641
TCACGAC2550.041.4705925
CACGACG2500.041.426
CTATGCG1200.041.2499961
AGGGCGA13850.041.101086
TATACGG1700.041.029412
ACATACG4450.040.9550617
TCGAACG556.184564E-1140.90909228
TAGCACG501.0822987E-940.51
CGGTTTT16750.040.2985081
TAGTACG451.929402E-840.0000041
TAGGGCG5900.039.661025
GCGATCG1250.039.6000029
TAGGGAC19650.039.0458035
GGGCGAT27850.039.0215457
Produced by FastQC (version 0.11.5)