##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547887_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1876652
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.478646014285015	31.0	31.0	33.0	30.0	34.0
2	31.831995489840416	31.0	31.0	34.0	30.0	34.0
3	31.55947186798618	31.0	31.0	34.0	30.0	34.0
4	35.52398899742733	37.0	35.0	37.0	33.0	37.0
5	35.56804031860995	37.0	35.0	37.0	33.0	37.0
6	35.66589490219817	37.0	35.0	37.0	33.0	37.0
7	36.02592702323073	37.0	35.0	37.0	35.0	37.0
8	36.0673337411518	37.0	35.0	37.0	35.0	37.0
9	37.823614607290004	39.0	38.0	39.0	35.0	39.0
10	37.39218619115318	39.0	37.0	39.0	35.0	39.0
11	37.10560775252951	39.0	37.0	39.0	33.0	39.0
12	36.72187651200116	39.0	35.0	39.0	33.0	39.0
13	36.607853240771334	39.0	35.0	39.0	33.0	39.0
14	37.743372239498854	40.0	36.0	41.0	33.0	41.0
15	37.87665161148684	40.0	36.0	41.0	33.0	41.0
16	37.94976319530739	40.0	36.0	41.0	33.0	41.0
17	37.86971372422804	40.0	36.0	41.0	33.0	41.0
18	37.674372233104485	39.0	36.0	41.0	33.0	41.0
19	37.49751259157265	39.0	36.0	41.0	33.0	41.0
20	37.24237418551761	39.0	35.0	41.0	33.0	41.0
21	37.129285024607654	39.0	35.0	41.0	32.0	41.0
22	37.041900682705155	39.0	35.0	41.0	32.0	41.0
23	36.932921500629845	38.0	35.0	41.0	32.0	41.0
24	36.90811775438387	38.0	35.0	41.0	32.0	41.0
25	36.82524836783804	38.0	35.0	41.0	32.0	41.0
26	36.703964826723336	38.0	35.0	41.0	32.0	41.0
27	36.60532640041947	38.0	35.0	41.0	31.0	41.0
28	36.5051320116889	38.0	35.0	40.0	31.0	41.0
29	36.47590709412294	38.0	35.0	40.0	31.0	41.0
30	36.33341983489747	38.0	35.0	40.0	31.0	41.0
31	36.11498082755887	38.0	35.0	40.0	30.0	41.0
32	35.8318036588563	38.0	35.0	40.0	30.0	41.0
33	35.577679825561695	38.0	35.0	40.0	29.0	41.0
34	35.30329331170616	38.0	35.0	40.0	26.0	41.0
35	35.07879670818031	38.0	34.0	40.0	25.0	41.0
36	34.91617732003589	38.0	34.0	40.0	24.0	41.0
37	34.78082191050871	38.0	34.0	40.0	23.0	41.0
38	34.64360147752487	37.0	34.0	40.0	23.0	41.0
39	34.557388903217	37.0	34.0	40.0	23.0	41.0
40	34.42041518619328	37.0	34.0	40.0	22.0	41.0
41	34.32880416827414	37.0	34.0	40.0	22.0	41.0
42	34.24016120197032	37.0	34.0	40.0	21.0	41.0
43	34.16159255951556	37.0	33.0	40.0	21.0	41.0
44	33.984459025967524	36.0	33.0	40.0	21.0	41.0
45	33.843289539030145	36.0	33.0	40.0	20.0	41.0
46	33.80549883515963	36.0	33.0	40.0	20.0	41.0
47	33.74316388973555	36.0	33.0	40.0	20.0	41.0
48	33.631210261678774	36.0	33.0	40.0	20.0	41.0
49	33.488996894469516	36.0	33.0	40.0	19.0	41.0
50	33.42108286459077	35.0	33.0	40.0	19.0	41.0
51	33.133100329736145	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	6.0
12	5.0
13	6.0
14	15.0
15	63.0
16	146.0
17	357.0
18	807.0
19	1674.0
20	2908.0
21	4740.0
22	7447.0
23	11425.0
24	17999.0
25	27986.0
26	37483.0
27	41614.0
28	41069.0
29	41742.0
30	45237.0
31	52589.0
32	63242.0
33	82204.0
34	133297.0
35	212518.0
36	158386.0
37	171012.0
38	251856.0
39	468741.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.215564739759955	23.126450721817367	27.82082133501576	18.837163203406917
2	36.21912853315372	24.316335687170557	27.732952087014535	11.731583692661186
3	27.554069694327982	23.715744847739483	36.207725246875825	12.522460211056712
4	23.75773451870672	26.52532275563077	36.24310740616801	13.473835319494503
5	21.72123547679591	30.48556684990078	34.64526188126515	13.147935792038162
6	19.90864582245403	38.19354893714977	31.82044406741367	10.077361172982524
7	82.42353936691512	3.5347523142276778	12.380345423658728	1.6613628951984705
8	82.89832105259792	2.963415699874031	12.173700824660086	1.9645624228679586
9	78.21871076789942	5.588622717477721	13.58253954382592	2.6101269707969297
10	39.066699633176526	32.55574288680054	19.18149981989202	9.196057660130915
11	28.663332359968713	24.547811741335103	31.17850299362908	15.61035290506711
12	27.848690114096808	23.720700481495772	32.06252411208897	16.368085292318447
13	21.393044634807094	30.874557456576923	32.06859875991926	15.663799148696722
14	16.39781909485616	34.38122784618565	32.649633496247574	16.571319562710617
15	14.993882723062136	29.250601603280735	40.4682914040536	15.287224269603527
16	18.496023769990387	27.25278847649964	38.69651912022048	15.554668633289495
17	18.054492788220724	27.014811483429003	32.133768008133636	22.796927720216644
18	19.71452352380729	28.627950200676523	34.73744732640894	16.92007894910724
19	22.566996971201906	30.144001125408444	30.29608046670347	16.992921436686185
20	23.63693428509921	29.9666640378717	29.89344854560142	16.50295313142767
21	20.940536657835338	29.90703657364285	32.38527974286122	16.76714702566059
22	20.561830323363097	27.03191641284586	32.082453219883064	20.32380004390798
23	17.194716974697492	31.420263319997527	31.83360580437929	19.55141390092569
24	18.072024008713388	29.6596811769044	35.60159262345922	16.666702190922983
25	18.167086918618903	31.077578581431187	33.316299452429114	17.439035047520797
26	17.401574719234038	34.158544045459685	29.96634431956484	18.473536915741438
27	16.99340101414647	32.920701334077926	32.682990772929664	17.402906878845943
28	15.730407129291951	31.268237265086974	36.22594919036668	16.7754064152544
29	17.162052421013595	29.470514512013946	35.147912346028996	18.219520720943468
30	18.086784337213295	30.847328114109594	34.03598536116446	17.029902187512654
31	22.764582884839598	30.000927183089885	30.198566383112052	17.035923548958465
32	22.82794039598178	30.884841728780827	30.094231642307683	16.192986232929705
33	21.15320261827979	31.142960975183463	29.915509108774565	17.788327297762187
34	18.266998889511747	31.130118956524704	31.91593326839499	18.686948885568555
35	17.826427062662656	31.640176228730738	31.72735275373378	18.806043954872827
36	22.116780308762625	31.474988436854566	29.601545731440886	16.80668552294192
37	19.05664982106432	33.26056189426703	30.681873890311046	17.00091439435761
38	18.43746203345106	34.17900601709854	28.872161700730874	18.511370248719526
39	18.509345366109432	32.738834903860706	29.26669409139254	19.485125638637317
40	20.507051920121576	31.3008485323864	29.55641216378956	18.635687383702464
41	17.539160163951546	30.28680863580461	30.555904877409347	21.618126322834495
42	20.061897464207533	31.42015674722857	28.42929855934931	20.08864722921458
43	19.927722348096506	31.363140315839054	29.113016158563227	19.59612117750121
44	19.223702636397157	33.28198302082645	29.088557708088658	18.40575663468773
45	17.896445371864363	34.662846388142285	27.771371570221863	19.669336669771486
46	19.999232676063546	32.69892340188804	28.686511937215855	18.615331984832565
47	19.228072119924207	32.009664018688596	29.6717771861805	19.090486675206698
48	19.451981507493134	30.748695016444177	30.518444549122588	19.280878926940105
49	19.17942165089745	29.76987742000115	30.653525533769717	20.397175395331686
50	17.66539560877563	31.859609560003666	31.131824120827943	19.343170710392762
51	17.345037865304807	32.67142762749833	29.848474837103524	20.135059670093337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1971.0
1	2617.0
2	3263.0
3	36609.5
4	69956.0
5	46908.0
6	23860.0
7	21789.0
8	19718.0
9	22614.0
10	25510.0
11	28447.0
12	31384.0
13	31402.5
14	31421.0
15	29440.5
16	27460.0
17	25025.0
18	22590.0
19	20496.5
20	18403.0
21	18267.0
22	18131.0
23	17694.0
24	17257.0
25	17500.0
26	20286.0
27	22829.0
28	26249.0
29	29669.0
30	34492.5
31	39316.0
32	44186.5
33	49057.0
34	54173.5
35	59290.0
36	63850.0
37	68410.0
38	72702.5
39	76995.0
40	90154.5
41	103314.0
42	116569.0
43	129824.0
44	133645.5
45	137467.0
46	141732.0
47	145997.0
48	146715.5
49	147434.0
50	137123.5
51	126813.0
52	113620.0
53	100427.0
54	87691.0
55	74955.0
56	70498.0
57	66041.0
58	58501.0
59	50961.0
60	45206.0
61	39451.0
62	33863.0
63	28275.0
64	24222.0
65	20169.0
66	16073.0
67	11977.0
68	9758.0
69	7539.0
70	6134.5
71	4730.0
72	3740.0
73	2750.0
74	2393.5
75	1534.5
76	1032.0
77	810.0
78	588.0
79	438.5
80	289.0
81	234.5
82	180.0
83	124.5
84	69.0
85	59.0
86	49.0
87	36.0
88	23.0
89	20.5
90	18.0
91	12.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1876652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.007218338185616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63850607807552	18.665014179304293
2	8.143503398904198	4.072927350683079
3	3.651379501064541	2.739325332560887
4	2.1298610324535527	2.1304759947423775
5	1.3809030515108245	1.7266272056498986
6	0.9349535780185835	1.4028352956947145
7	0.7436350411782687	1.3017370687069434
8	0.5646395376848642	1.1296051361006068
9	0.4655171304662551	1.0477159669561753
>10	5.890659299779292	33.95759095898709
>50	1.3101184330936895	22.59529922967744
>100	0.14113666423331866	4.7215511789183395
>500	0.0025936267685271823	0.45250235043086023
>1k	0.0021613556404393186	0.7919331627464123
>5k	2.161355640439319E-4	0.29143486305819377
>10k+	2.161355640439319E-4	2.973424725782718
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55013	2.9314438691883207	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5392	0.2873201850955851	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT	2228	0.11872206461293835	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2006	0.10689248725922547	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05936103230646918	0.0
2	0.0	0.0	0.0	0.1775502330746457	0.0
3	0.0	0.0	0.0	0.3281375556043422	0.0
4	0.0	0.0	0.0	0.4743553946069916	0.0
5	0.0	0.0	0.0	0.8622269872091363	0.0
6	0.0	0.0	0.0	1.5146121923510592	0.0
7	0.0	0.0	0.0	1.969624629393196	0.0
8	0.0	0.0	0.0	2.7068417586212044	0.0
9	0.0	0.0	0.0	3.18295560391591	0.0
10	0.0	0.0	0.0	3.731538932098226	0.0
11	0.0	0.0	0.0	4.192572730586171	0.0
12	0.0	0.0	0.0	4.531580708623656	0.0
13	0.0	0.0	0.0	4.7060403313986825	0.0
14	0.0	0.0	0.0	4.808616621515337	0.0
15	0.0	0.0	0.0	4.898457465742183	0.0
16	0.0	0.0	0.0	5.055385868024546	0.0
17	0.0	0.0	0.0	5.240929058770619	0.0
18	0.0	0.0	0.0	5.482316380447734	0.0
19	0.0	0.0	0.0	5.615745487176099	0.0
20	0.0	0.0	0.0	5.759299006954939	0.0
21	0.0	0.0	0.0	5.923580930295015	0.0
22	0.0	0.0	0.0	6.102196891059184	0.0
23	0.0	0.0	0.0	6.304791724837636	0.0
24	0.0	0.0	0.0	6.459055807896189	0.0
25	0.0	0.0	0.0	6.59520252023284	0.0
26	0.0	0.0	0.0	6.719093364139969	0.0
27	0.0	0.0	0.0	6.8585971186986185	0.0
28	0.0	0.0	0.0	7.002310497630887	0.0
29	0.0	0.0	0.0	7.163128805980011	0.0
30	0.0	0.0	0.0	7.372917301662748	0.0
31	0.0	0.0	0.0	7.530644999712253	0.0
32	0.0	0.0	0.0	7.691676453599282	0.0
33	0.0	0.0	0.0	7.849244292495359	0.0
34	0.0	0.0	0.0	8.016616826135053	0.0
35	0.0	0.0	0.0	8.213296871236649	0.0
36	0.0	0.0	0.0	8.388502503394342	0.0
37	0.0	0.0	0.0	8.563068698938322	0.0
38	0.0	0.0	0.0	8.752981373211442	0.0
39	0.0	0.0	0.0	9.027566112417219	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	20	7.0344284E-4	45.000004	8
AATGCGT	20	7.0344284E-4	45.000004	17
CGTTTTT	16695	0.0	43.948784	1
TAGTGCG	145	0.0	43.448273	1
CGACGGT	240	0.0	43.124996	28
TAATACG	65	0.0	41.538464	1
TCACGAC	255	0.0	41.47059	25
CACGACG	250	0.0	41.4	26
CTATGCG	120	0.0	41.249996	1
AGGGCGA	1385	0.0	41.10108	6
TATACGG	170	0.0	41.02941	2
ACATACG	445	0.0	40.95506	17
TCGAACG	55	6.184564E-11	40.909092	28
TAGCACG	50	1.0822987E-9	40.5	1
CGGTTTT	1675	0.0	40.298508	1
TAGTACG	45	1.929402E-8	40.000004	1
TAGGGCG	590	0.0	39.66102	5
GCGATCG	125	0.0	39.600002	9
TAGGGAC	1965	0.0	39.045803	5
GGGCGAT	2785	0.0	39.021545	7
>>END_MODULE