FastQCFastQC Report
Thu 26 May 2016
SRR1547886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547886_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1197820
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47980.4005610191848525No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.39830692424571307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA302.1655778E-645.00000441
GTCGATG302.1655778E-645.0000041
TAGCGAT302.1655778E-645.00000413
CGTCTAG302.1655778E-645.00000439
ATCGTAG302.1655778E-645.0000041
TTGCGTT302.1655778E-645.0000041
AACTGTG302.1655778E-645.00000424
CGGTGCC302.1655778E-645.00000420
TCGACCG302.1655778E-645.00000415
GCGTATT302.1655778E-645.00000440
TCTTGCG207.033405E-445.01
ACCGGTC253.8909046E-545.023
TCGCAAT453.8562575E-1045.022
CGAGTCA207.033405E-445.042
TTTACGC253.8909046E-545.025
TCCGATT453.8562575E-1045.030
GCGGTAT351.2119926E-745.09
CGGTTGA551.8189894E-1245.042
CACATCG253.8909046E-545.019
TTAGACA253.8909046E-545.024
Produced by FastQC (version 0.11.5)