##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547886_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1197820
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.589426625035482	31.0	31.0	33.0	30.0	34.0
2	32.00626972333072	31.0	31.0	34.0	30.0	34.0
3	32.07950192850345	33.0	31.0	34.0	30.0	34.0
4	35.83136030455327	37.0	35.0	37.0	35.0	37.0
5	35.72564158220768	37.0	35.0	37.0	35.0	37.0
6	35.78098378721344	37.0	35.0	37.0	35.0	37.0
7	36.044580153946335	37.0	35.0	37.0	35.0	37.0
8	36.073946836753436	37.0	35.0	37.0	35.0	37.0
9	37.907653904593346	39.0	38.0	39.0	35.0	39.0
10	37.506629543671	39.0	37.0	39.0	35.0	39.0
11	37.14256148670084	39.0	37.0	39.0	34.0	39.0
12	35.83742381993956	37.0	35.0	39.0	33.0	39.0
13	35.28786962982752	37.0	35.0	39.0	31.0	39.0
14	36.19284366599322	37.0	35.0	41.0	31.0	41.0
15	36.58240553672505	37.0	35.0	41.0	32.0	41.0
16	36.7985999565878	37.0	35.0	41.0	33.0	41.0
17	36.74714898732698	37.0	35.0	41.0	33.0	41.0
18	36.697997194904076	37.0	35.0	40.0	32.0	41.0
19	36.58477567581106	37.0	35.0	40.0	32.0	41.0
20	36.41081631630796	36.0	35.0	40.0	32.0	41.0
21	36.2360555008265	36.0	35.0	40.0	32.0	41.0
22	36.11577616002404	35.0	35.0	40.0	31.0	41.0
23	36.10970596583794	35.0	35.0	40.0	32.0	41.0
24	36.068732363794226	35.0	35.0	40.0	32.0	41.0
25	36.00517440016029	35.0	35.0	40.0	32.0	41.0
26	35.88332554140021	35.0	35.0	40.0	31.0	41.0
27	35.776692658329296	35.0	35.0	40.0	31.0	41.0
28	35.792631614098944	36.0	35.0	40.0	31.0	41.0
29	35.86447212435925	36.0	35.0	40.0	31.0	41.0
30	35.85268487752751	36.0	35.0	40.0	31.0	41.0
31	35.61235828421633	35.0	35.0	40.0	31.0	41.0
32	35.36505484964352	35.0	34.0	40.0	30.0	41.0
33	35.240296538711995	35.0	34.0	40.0	30.0	41.0
34	35.1579110383864	35.0	34.0	40.0	30.0	41.0
35	35.08819522131873	35.0	34.0	40.0	30.0	41.0
36	34.86851446795011	35.0	34.0	40.0	29.0	41.0
37	34.69919520462173	35.0	34.0	40.0	27.0	41.0
38	34.63555125144011	35.0	34.0	40.0	27.0	41.0
39	34.6240962748994	35.0	34.0	40.0	27.0	41.0
40	34.406915897213274	35.0	34.0	40.0	26.0	41.0
41	34.41435357566245	35.0	34.0	40.0	26.0	41.0
42	34.32198243475647	35.0	34.0	40.0	26.0	41.0
43	34.207784141189826	35.0	34.0	40.0	26.0	41.0
44	33.9512481007163	35.0	34.0	40.0	24.0	41.0
45	33.82431083134361	35.0	34.0	39.0	23.0	41.0
46	33.82907532016497	35.0	34.0	39.0	23.0	41.0
47	33.783837304436396	35.0	33.0	39.0	23.0	41.0
48	33.75678482576681	35.0	33.0	39.0	23.0	41.0
49	33.70823579502763	35.0	34.0	39.0	24.0	41.0
50	33.55609023058556	35.0	33.0	39.0	24.0	41.0
51	33.17619926199262	35.0	33.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	5.0
14	11.0
15	32.0
16	91.0
17	200.0
18	490.0
19	982.0
20	1740.0
21	2782.0
22	4286.0
23	6511.0
24	9560.0
25	13884.0
26	17857.0
27	20410.0
28	22452.0
29	25499.0
30	30233.0
31	36875.0
32	46640.0
33	64109.0
34	137314.0
35	254127.0
36	67889.0
37	79890.0
38	125328.0
39	228571.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.775709205055854	24.532901437611663	29.449165984872515	13.242223372459968
2	28.936150673723933	26.864470454659294	31.198510627640214	13.000868243976557
3	28.685111285501996	25.914077240319916	31.341269973785714	14.059541500392381
4	24.927535021956555	28.401262293165917	31.263545440884272	15.407657243993256
5	23.807667262192982	32.94134344058372	29.686179893473142	13.564809403750147
6	20.74902739977626	41.555826417992684	26.831076455560936	10.864069726670117
7	88.28096041141407	4.582908951261459	5.728907515319497	1.4072231220049758
8	89.3709405419846	3.419795962665509	5.003589854903074	2.2056736404468116
9	86.42425406154514	4.906329832529095	6.351037718521982	2.3183783874037833
10	58.9571888931559	21.780985456913392	10.151358300913325	9.110467349017382
11	54.015795361573524	17.59554857992019	16.727555058356014	11.661101000150273
12	48.278622831477186	20.477367217111084	19.292798584094438	11.951211367317294
13	20.446394282947356	48.32337079026899	19.632248584929286	11.59798634185437
14	12.99243625920422	49.98897998029754	24.16982518241472	12.848758578083519
15	11.768713162244744	23.186622363961195	53.13628091032041	11.908383563473643
16	13.786629042761017	17.713178941744168	51.1610258636523	17.339166151842512
17	14.524970362825801	17.621679384214655	26.444373946001903	41.40897630695764
18	21.660015695179577	23.622664507188055	33.983737122439095	20.73358267519327
19	29.55936618189711	26.465328680436123	23.344325524703212	20.63097961296355
20	33.70823662987761	23.445342371975755	22.840326593311183	20.00609440483545
21	20.8674926115777	29.152209847890337	27.677280392713428	22.303017147818537
22	22.024845135329183	24.597602310864737	25.592576514000438	27.78497603980565
23	19.22567664590673	32.058406104423035	21.89010034896729	26.825816900702943
24	20.851463492010485	24.368936902038705	38.08326793675177	16.696331669199044
25	15.479203887061496	25.905311315556595	36.334006778981816	22.281478018400094
26	15.079143777863118	38.75139837371224	25.56995207961129	20.599505768813344
27	16.24826768629677	36.93743634268922	28.89006695496819	17.924229016045818
28	12.769948740211385	30.388288724516205	41.647910370506416	15.193852164765993
29	13.031841178140288	25.385867659581574	40.87250171144245	20.709789450835686
30	16.976841261625285	32.56449216075871	32.49094187774457	17.967724699871432
31	30.841027867292247	28.369287539029237	22.240904309495583	18.548780284182932
32	30.932193484830776	29.605449900652854	23.51905962498539	15.94329698953098
33	27.468150473359938	31.45622881568182	22.32088293733616	18.75473777362208
34	17.647643218513632	31.355211968409275	26.338932393848825	24.658212419228263
35	17.659832028184535	28.270858726686814	30.222737973986074	23.846571271142576
36	32.24808401930173	23.67500960077474	26.312801589554358	17.76410479036917
37	19.790285685662287	32.362291496218134	30.408074668981982	17.4393481491376
38	19.06071029036082	33.95318161326409	22.218864270090666	24.767243826284417
39	19.519126412983585	31.854285284934296	28.417458382728622	20.20912991935349
40	23.632348766926583	27.458048788632684	24.15304469786779	24.75655774657294
41	15.776243509041427	23.519977959960595	28.12300679567882	32.58077173531916
42	22.97206591975422	25.398306924245713	23.274532066587632	28.35509508941243
43	23.671503230869412	26.607420146599658	24.64109799469035	25.07997862784058
44	18.90217227964135	33.631513916949125	26.769130587233477	20.697183216176054
45	17.13496184735603	40.01736487953115	20.130153111485864	22.717520161626954
46	23.838974136347698	33.51872568499441	23.534671319563873	19.10762885909402
47	21.762702242407038	29.093519894474962	25.100682907281563	24.043094955836438
48	24.543253577332152	22.65991551318228	30.480539647025427	22.316291262460137
49	20.536057170526455	21.755689502596383	31.700839859077323	26.007413467799832
50	18.30767561069276	33.559048938905676	27.13537927234476	20.997896178056802
51	17.497620677564242	36.35487802841829	22.69639845719724	23.451102836820223
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1133.0
1	1350.0
2	1567.0
3	4541.5
4	7516.0
5	5996.5
6	4477.0
7	4757.0
8	5037.0
9	5608.5
10	6180.0
11	6272.5
12	6365.0
13	6484.5
14	6604.0
15	6172.0
16	5740.0
17	5628.0
18	5516.0
19	5181.5
20	4847.0
21	4860.5
22	4874.0
23	5056.0
24	5238.0
25	5290.0
26	5896.0
27	6450.0
28	6740.0
29	7030.0
30	9242.5
31	11455.0
32	14735.5
33	18016.0
34	21050.5
35	24085.0
36	25502.0
37	26919.0
38	34355.0
39	41791.0
40	67538.5
41	93286.0
42	119563.5
43	145841.0
44	144163.0
45	142485.0
46	134823.0
47	127161.0
48	114826.5
49	102492.0
50	94731.0
51	86970.0
52	79323.0
53	71676.0
54	62883.5
55	54091.0
56	46893.0
57	39695.0
58	38206.0
59	36717.0
60	33348.0
61	29979.0
62	25267.0
63	20555.0
64	18254.5
65	15954.0
66	12565.0
67	9176.0
68	7555.5
69	5935.0
70	5277.0
71	4619.0
72	3239.5
73	1860.0
74	1595.0
75	1149.0
76	968.0
77	662.5
78	357.0
79	303.0
80	249.0
81	191.5
82	134.0
83	89.0
84	44.0
85	33.5
86	23.0
87	20.0
88	17.0
89	15.5
90	14.0
91	10.5
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1197820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.60620483941674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8092726707604	14.227446075656882
2	7.786271915754181	2.741733965683318
3	2.4240915464427784	1.2803715694851014
4	1.1409398970014701	0.8035048614428368
5	0.6611532300202002	0.5820199598988828
6	0.4231175917109354	0.4469696994494057
7	0.30437365825788937	0.37512054825077196
8	0.24231413289413445	0.34129858073758246
9	0.18148570936920091	0.28757471171229043
>10	2.322818388057758	10.614389595491817
>50	2.0197527802738273	26.723087054540258
>100	1.6786432479246043	40.249997266380404
>500	0.004804359610545519	0.5170775155079887
>1k	9.608719221091039E-4	0.8094085957624644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4798	0.4005610191848525	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4771	0.39830692424571307	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6696999549181013E-4	0.0	0.0	0.05760464844467449	0.0
2	1.6696999549181013E-4	0.0	0.0	0.22348933896578785	0.0
3	1.6696999549181013E-4	0.0	0.0	0.32475664123157066	0.0
4	1.6696999549181013E-4	0.0	0.0	0.5866490791604748	0.0
5	1.6696999549181013E-4	0.0	0.0	0.9498088193551619	0.0
6	1.6696999549181013E-4	0.0	0.0	1.409644186939607	0.0
7	1.6696999549181013E-4	0.0	0.0	1.606668781619943	0.0
8	1.6696999549181013E-4	0.0	0.0	2.132457297423653	0.0
9	1.6696999549181013E-4	0.0	0.0	2.298425472942512	0.0
10	1.6696999549181013E-4	0.0	0.0	2.6832913125511344	0.0
11	1.6696999549181013E-4	0.0	0.0	3.1104840460169307	0.0
12	1.6696999549181013E-4	0.0	0.0	3.477901521096659	0.0
13	1.6696999549181013E-4	0.0	0.0	3.620076472257935	0.0
14	1.6696999549181013E-4	0.0	0.0	3.6822727955786347	0.0
15	1.6696999549181013E-4	0.0	0.0	3.7810355479120403	0.0
16	1.6696999549181013E-4	0.0	0.0	3.9892471322903273	0.0
17	2.5045499323771516E-4	0.0	0.0	4.2298508957940255	0.0
18	2.5045499323771516E-4	0.0	0.0	4.578734701374163	0.0
19	2.5045499323771516E-4	0.0	0.0	4.726252692391177	0.0
20	2.5045499323771516E-4	0.0	0.0	4.861665358735035	0.0
21	2.5045499323771516E-4	0.0	0.0	5.042243408859428	0.0
22	2.5045499323771516E-4	0.0	0.0	5.2130537142475495	0.0
23	2.5045499323771516E-4	0.0	0.0	5.42385333355596	0.0
24	2.5045499323771516E-4	0.0	0.0	5.571120869579737	0.0
25	2.5045499323771516E-4	0.0	0.0	5.67748075670802	0.0
26	2.5045499323771516E-4	0.0	0.0	5.791855203619909	0.0
27	2.5045499323771516E-4	0.0	0.0	5.891202350937537	0.0
28	2.5045499323771516E-4	0.0	0.0	5.998146633050041	0.0
29	2.5045499323771516E-4	0.0	0.0	6.118698969795128	0.0
30	2.5045499323771516E-4	0.0	0.0	6.286503815264397	0.0
31	2.5045499323771516E-4	0.0	0.0	6.4503013808418626	0.0
32	2.5045499323771516E-4	0.0	0.0	6.573191297523835	0.0
33	2.5045499323771516E-4	0.0	0.0	6.693242724282446	0.0
34	2.5045499323771516E-4	0.0	0.0	6.833664490491059	0.0
35	2.5045499323771516E-4	0.0	0.0	7.0465512347431165	0.0
36	2.5045499323771516E-4	0.0	0.0	7.2067589454175085	0.0
37	2.5045499323771516E-4	0.0	0.0	7.360204371274482	0.0
38	2.5045499323771516E-4	0.0	0.0	7.505468267352357	0.0
39	2.5045499323771516E-4	0.0	0.0	7.663505368085355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	30	2.1655778E-6	45.000004	41
GTCGATG	30	2.1655778E-6	45.000004	1
TAGCGAT	30	2.1655778E-6	45.000004	13
CGTCTAG	30	2.1655778E-6	45.000004	39
ATCGTAG	30	2.1655778E-6	45.000004	1
TTGCGTT	30	2.1655778E-6	45.000004	1
AACTGTG	30	2.1655778E-6	45.000004	24
CGGTGCC	30	2.1655778E-6	45.000004	20
TCGACCG	30	2.1655778E-6	45.000004	15
GCGTATT	30	2.1655778E-6	45.000004	40
TCTTGCG	20	7.033405E-4	45.0	1
ACCGGTC	25	3.8909046E-5	45.0	23
TCGCAAT	45	3.8562575E-10	45.0	22
CGAGTCA	20	7.033405E-4	45.0	42
TTTACGC	25	3.8909046E-5	45.0	25
TCCGATT	45	3.8562575E-10	45.0	30
GCGGTAT	35	1.2119926E-7	45.0	9
CGGTTGA	55	1.8189894E-12	45.0	42
CACATCG	25	3.8909046E-5	45.0	19
TTAGACA	25	3.8909046E-5	45.0	24
>>END_MODULE