FastQCFastQC Report
Thu 26 May 2016
SRR1547885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547885_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1875191
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT485102.5869364774041683No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.24418845866901023No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTT29790.15886381707250088No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT23160.12350741871094732No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22100.11785466120517857No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC20930.11161529678843382No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG1250.045.00000421
ACGCTTA302.1662381E-644.99999623
CGTTTTT147600.043.2469521
CGACGGT3350.042.31343528
GACGGTC3350.042.31343529
GTCAACG700.041.7857131
CTCACGA3450.041.73913224
CCGGATA1000.040.520
TTGTACG950.040.2631571
CGGTCTA3600.040.031
ACGAACT451.929402E-840.021
GCCGGAT850.039.70588319
TCGACAG850.039.7058831
CTATACG403.4597724E-739.3750041
TCCGTAC403.4597724E-739.37500413
CGGGTAT1200.039.3749966
GGAACGC1200.039.3749968
CGGGACC4300.039.2441866
TACGGGA5800.039.1810344
AAGGGAC17950.038.732595
Produced by FastQC (version 0.11.5)