##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547885_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1875191
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426995436731513	31.0	31.0	33.0	30.0	34.0
2	31.791828139106897	31.0	31.0	34.0	30.0	34.0
3	31.592746018938872	31.0	31.0	34.0	30.0	34.0
4	35.51846505236	37.0	35.0	37.0	33.0	37.0
5	35.52501105220748	37.0	35.0	37.0	33.0	37.0
6	35.60659687466504	37.0	35.0	37.0	33.0	37.0
7	35.97268064959783	37.0	35.0	37.0	35.0	37.0
8	36.008844432380485	37.0	35.0	37.0	35.0	37.0
9	37.75587020202209	39.0	38.0	39.0	35.0	39.0
10	37.3296618851093	39.0	37.0	39.0	34.0	39.0
11	37.010645848876194	39.0	37.0	39.0	33.0	39.0
12	36.45647083417103	39.0	35.0	39.0	32.0	39.0
13	36.26609556039892	39.0	35.0	39.0	32.0	39.0
14	37.32623556747019	40.0	35.0	41.0	32.0	41.0
15	37.53390827920996	40.0	35.0	41.0	33.0	41.0
16	37.63596561630255	40.0	35.0	41.0	33.0	41.0
17	37.56744779598451	40.0	35.0	41.0	33.0	41.0
18	37.39750777387477	39.0	35.0	41.0	33.0	41.0
19	37.24634930521744	39.0	35.0	41.0	32.0	41.0
20	36.99413446416925	38.0	35.0	41.0	32.0	41.0
21	36.8812355648038	38.0	35.0	41.0	32.0	41.0
22	36.793881796574325	38.0	35.0	41.0	32.0	41.0
23	36.704818869117865	38.0	35.0	41.0	32.0	41.0
24	36.65058172740803	38.0	35.0	41.0	32.0	41.0
25	36.55859749753492	38.0	35.0	40.0	32.0	41.0
26	36.41759852729668	38.0	35.0	40.0	31.0	41.0
27	36.32312388444697	38.0	35.0	40.0	31.0	41.0
28	36.24384662682361	37.0	35.0	40.0	31.0	41.0
29	36.20198955733043	37.0	35.0	40.0	31.0	41.0
30	36.07062747208151	37.0	35.0	40.0	31.0	41.0
31	35.86208018276538	37.0	35.0	40.0	30.0	41.0
32	35.58484655696407	37.0	35.0	40.0	30.0	41.0
33	35.37648484874341	37.0	34.0	40.0	29.0	41.0
34	35.143285137354006	37.0	34.0	40.0	27.0	41.0
35	34.95395882339452	37.0	34.0	40.0	25.0	41.0
36	34.790579732944536	37.0	34.0	40.0	24.0	41.0
37	34.64851473796536	37.0	34.0	40.0	24.0	41.0
38	34.519091655196725	36.0	34.0	40.0	23.0	41.0
39	34.43441654743437	36.0	34.0	40.0	23.0	41.0
40	34.28528827196803	36.0	34.0	40.0	23.0	41.0
41	34.204631421545855	36.0	34.0	40.0	22.0	41.0
42	34.12147029289283	36.0	33.0	40.0	22.0	41.0
43	34.03435063414874	36.0	33.0	40.0	22.0	41.0
44	33.85571549778129	35.0	33.0	40.0	21.0	41.0
45	33.730334669908295	35.0	33.0	40.0	21.0	41.0
46	33.69704686082645	35.0	33.0	40.0	21.0	41.0
47	33.61882176268978	35.0	33.0	40.0	20.0	41.0
48	33.50939131000522	35.0	33.0	40.0	20.0	41.0
49	33.37743728505523	35.0	33.0	40.0	20.0	41.0
50	33.29102582083638	35.0	33.0	40.0	20.0	41.0
51	32.9990059679254	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	7.0
13	9.0
14	24.0
15	59.0
16	168.0
17	428.0
18	940.0
19	1808.0
20	3251.0
21	5317.0
22	8020.0
23	12204.0
24	18116.0
25	27537.0
26	36269.0
27	39790.0
28	40770.0
29	42935.0
30	47779.0
31	55254.0
32	68111.0
33	88277.0
34	151581.0
35	241102.0
36	162527.0
37	163932.0
38	233911.0
39	424995.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.19259958052273	25.083471497036836	27.31199115183467	18.411937770605768
2	33.67998246578615	25.838594575165942	28.031064568889246	12.450358390158655
3	27.13200948596703	25.220577530502226	35.31725568222117	12.330157301309573
4	24.190069171620383	26.777378944331538	35.38114250761656	13.65140937643152
5	22.37078782907981	30.519771052655436	33.6298009109472	13.479640207317548
6	19.834512857623572	39.49555005330124	30.33221682484611	10.337720264229084
7	83.27455709845025	3.654401071677498	11.237735249369264	1.8333065805029993
8	83.6372401531364	3.272839940038108	10.850787999729095	2.239131907096397
9	79.38343347424343	5.440512459797429	12.405083002211507	2.7709710637476395
10	43.137205756640256	31.073634632418777	16.821433123345837	8.967726487595131
11	32.98762632713147	23.63897864270893	28.91369465830414	14.459700371855453
12	31.285186415677124	23.527203362217502	29.712493287350462	15.475116934754912
13	20.960424831390508	34.0724224892291	29.728385001847812	15.238767677532582
14	15.05649291192204	36.891175352270785	31.775216497946072	16.277115237861103
15	14.281051903512761	28.409319370666775	42.39338819352268	14.916240532297776
16	17.434384017414757	26.618035176150055	40.04194772692488	15.9056330795103
17	17.260481732260875	27.5629522539304	30.1663137248419	25.010252288966832
18	19.90202598028681	29.307894502480014	33.47136371708269	17.318715800150493
19	22.693848253324596	31.475620350140332	27.948459650243628	17.882071746291444
20	24.04261752536142	30.455724243557057	28.28341219641092	17.2182460346706
21	19.902879226702773	31.874246410099023	30.546701642659336	17.67617272053887
22	20.016734295333116	28.29994384572025	30.41124877412488	21.27207308482176
23	17.288372224482732	32.134379911166384	29.706307250834712	20.87094061351617
24	17.821811218163912	30.5157714600806	35.11583620015241	16.54658112160308
25	17.69419755107613	31.292332354410828	32.406192222552264	18.607277871960775
26	16.039059487806842	35.70857581974316	28.804159149654623	19.448205542795375
27	15.985251635700044	34.599568790592535	31.57811657585814	17.837062997849284
28	15.205544395210941	32.54521806045357	35.072960567750165	17.17627697658532
29	16.04049934113378	30.15943442561318	34.25635041977057	19.543715813482468
30	18.399832337079264	32.10862253498444	32.09545054343798	17.396094584498325
31	22.85324534940707	31.512469929729825	27.391449724321415	18.242834996541685
32	23.428600073272534	31.809239698782683	27.52252970497405	17.239630522970728
33	21.149525568328773	32.83420195596075	26.90349943019138	19.112773045519095
34	16.944140623541813	32.82886916586097	29.65932537005564	20.56766484054158
35	16.659369632213465	33.194858550409	29.877169845631723	20.268601971745813
36	21.859266602708736	31.905549887984748	28.516401795870394	17.718781713436123
37	17.443129793178404	35.186655652677516	29.437747941409704	17.932466612734384
38	17.068181321262742	35.631996953910296	27.391289740618426	19.90853198420854
39	16.88361345590929	35.12623514084699	28.486164876004633	19.503986527239093
40	19.99732293936991	32.23191664209139	28.096444575512574	19.674315843026125
41	16.60321535246276	30.85744332177362	29.025469938795567	23.513871386968045
42	19.197831047610617	32.318414497509856	26.995916682620596	21.487837772258935
43	19.31755218535072	32.0801987637526	27.93390113327122	20.668347917625457
44	18.37130191004543	34.632472105508185	27.516770291666287	19.479455692780096
45	16.87049479226383	36.636267985501206	25.612537602836188	20.880699619398772
46	20.11757735611999	34.191237052652234	26.796576988690752	18.894608602537023
47	18.60647795344581	32.71517408093362	28.641509051611276	20.03683891400929
48	19.77478560850601	30.758840032828655	29.35988920595289	20.106485152712445
49	18.91908610909502	29.854932110915634	29.545256989821304	21.68072479016804
50	17.32148885100238	32.901448439119	29.812109806414387	19.964952903464233
51	16.556180143782687	34.411961234882206	27.626412456117805	21.405446165217302
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2330.0
1	2770.0
2	3210.0
3	31586.0
4	59962.0
5	39862.5
6	19763.0
7	18855.5
8	17948.0
9	21795.0
10	25642.0
11	29473.0
12	33304.0
13	33901.5
14	34499.0
15	31525.0
16	28551.0
17	24990.0
18	21429.0
19	19669.0
20	17909.0
21	16842.0
22	15775.0
23	16495.5
24	17216.0
25	17748.0
26	20360.5
27	22441.0
28	24862.0
29	27283.0
30	31429.0
31	35575.0
32	39484.0
33	43393.0
34	49213.0
35	55033.0
36	57991.0
37	60949.0
38	68123.5
39	75298.0
40	90447.5
41	105597.0
42	121467.5
43	137338.0
44	141620.0
45	145902.0
46	150632.0
47	155362.0
48	154979.0
49	154596.0
50	141898.5
51	129201.0
52	115046.0
53	100891.0
54	88801.5
55	76712.0
56	68538.5
57	60365.0
58	56259.5
59	52154.0
60	46268.0
61	40382.0
62	34810.0
63	29238.0
64	24884.0
65	20530.0
66	15833.5
67	11137.0
68	9364.0
69	7591.0
70	6470.0
71	5349.0
72	4233.5
73	3118.0
74	2468.0
75	1275.5
76	733.0
77	692.5
78	652.0
79	464.5
80	277.0
81	254.0
82	231.0
83	156.0
84	81.0
85	72.0
86	63.0
87	47.5
88	32.0
89	28.5
90	25.0
91	19.0
92	13.0
93	10.5
94	8.0
95	6.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1875191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.548103243286842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34728670249562	18.496329730743966
2	8.192415305792956	4.022165134769777
3	3.5673366840810634	2.6271404767315945
4	2.1942943627419136	2.1546305825100323
5	1.4002789832447076	1.7187096525047902
6	0.9856741851693166	1.4517858997067412
7	0.682994428956667	1.1736352429632602
8	0.5129610319729312	1.0073776298123582
9	0.4105797679539643	0.9071059080004803
>10	4.946945360438619	29.630577493246562
>50	1.5721594450591327	26.422143195066262
>100	0.18112440249382666	5.978110425774849
>500	0.0030848427551545972	0.4982839129538904
>1k	0.0026441509329896548	1.2880628331709718
>5k	0.0	0.0
>10k+	2.2034591108247123E-4	2.623941882044423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48510	2.5869364774041683	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4579	0.24418845866901023	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTT	2979	0.15886381707250088	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT	2316	0.12350741871094732	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2210	0.11785466120517857	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	2093	0.11161529678843382	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.332790099781835E-5	0.0	0.0	0.06996620610913769	0.0
2	5.332790099781835E-5	0.0	0.0	0.21971095211101163	0.0
3	5.332790099781835E-5	0.0	0.0	0.39553304170081877	0.0
4	5.332790099781835E-5	0.0	0.0	0.5772745283013837	0.0
5	5.332790099781835E-5	0.0	0.0	1.071837482155151	0.0
6	5.332790099781835E-5	0.0	0.0	1.8849279886688877	0.0
7	5.332790099781835E-5	0.0	0.0	2.479320773190571	0.0
8	5.332790099781835E-5	0.0	0.0	3.4589543145204944	0.0
9	5.332790099781835E-5	0.0	0.0	4.051960573616234	0.0
10	5.332790099781835E-5	0.0	0.0	4.7486362722517335	0.0
11	5.332790099781835E-5	0.0	0.0	5.338869480495587	0.0
12	5.332790099781835E-5	0.0	0.0	5.7858639466593	0.0
13	5.332790099781835E-5	0.0	0.0	5.9912830213028965	0.0
14	5.332790099781835E-5	0.0	0.0	6.104178187715278	0.0
15	5.332790099781835E-5	0.0	0.0	6.209554120086967	0.0
16	5.332790099781835E-5	0.0	0.0	6.398388217520242	0.0
17	5.332790099781835E-5	0.0	0.0	6.617405906918282	0.0
18	5.332790099781835E-5	0.0	0.0	6.917322022130012	0.0
19	5.332790099781835E-5	0.0	0.0	7.067120096032884	0.0
20	5.332790099781835E-5	0.0	0.0	7.237716051324905	0.0
21	5.332790099781835E-5	0.0	0.0	7.425750230243213	0.0
22	5.332790099781835E-5	0.0	0.0	7.626103154292016	0.0
23	5.332790099781835E-5	0.0	0.0	7.848747140957908	0.0
24	5.332790099781835E-5	0.0	0.0	8.022276130804809	0.0
25	5.332790099781835E-5	0.0	0.0	8.166581430904905	0.0
26	5.332790099781835E-5	0.0	0.0	8.302514250548343	0.0
27	5.332790099781835E-5	0.0	0.0	8.45807173775898	0.0
28	5.332790099781835E-5	0.0	0.0	8.608456418572828	0.0
29	5.332790099781835E-5	0.0	0.0	8.780385571389795	0.0
30	1.066558019956367E-4	0.0	0.0	9.002229639540719	0.0
31	1.066558019956367E-4	0.0	0.0	9.17917161505148	0.0
32	1.066558019956367E-4	0.0	0.0	9.33926197384693	5.332790099781835E-5
33	1.066558019956367E-4	0.0	0.0	9.497325872404465	5.332790099781835E-5
34	1.066558019956367E-4	0.0	0.0	9.671868092370325	5.332790099781835E-5
35	1.066558019956367E-4	0.0	0.0	9.885019712658604	5.332790099781835E-5
36	1.066558019956367E-4	0.0	0.0	10.05817540719852	5.332790099781835E-5
37	1.066558019956367E-4	0.0	0.0	10.240610156512057	5.332790099781835E-5
38	1.066558019956367E-4	0.0	0.0	10.45146867705743	5.332790099781835E-5
39	1.066558019956367E-4	0.0	0.0	10.775435675619176	5.332790099781835E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	125	0.0	45.000004	21
ACGCTTA	30	2.1662381E-6	44.999996	23
CGTTTTT	14760	0.0	43.246952	1
CGACGGT	335	0.0	42.313435	28
GACGGTC	335	0.0	42.313435	29
GTCAACG	70	0.0	41.785713	1
CTCACGA	345	0.0	41.739132	24
CCGGATA	100	0.0	40.5	20
TTGTACG	95	0.0	40.263157	1
CGGTCTA	360	0.0	40.0	31
ACGAACT	45	1.929402E-8	40.0	21
GCCGGAT	85	0.0	39.705883	19
TCGACAG	85	0.0	39.705883	1
CTATACG	40	3.4597724E-7	39.375004	1
TCCGTAC	40	3.4597724E-7	39.375004	13
CGGGTAT	120	0.0	39.374996	6
GGAACGC	120	0.0	39.374996	8
CGGGACC	430	0.0	39.244186	6
TACGGGA	580	0.0	39.181034	4
AAGGGAC	1795	0.0	38.73259	5
>>END_MODULE