FastQCFastQC Report
Thu 26 May 2016
SRR1547884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547884_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1711308
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86990.5083246265429717No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48560.28375955701720557No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT21500.1256348944783756No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17920.10471522367686004No Hit
AAGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT17250.100800089755906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC302.1661253E-645.00000418
GCGAAAC302.1661253E-645.00000443
CGTTGCA302.1661253E-645.00000419
ATTACGA302.1661253E-645.00000430
CCATGCG600.045.0000041
TGCGCAC302.1661253E-645.00000413
CGTAACA302.1661253E-645.00000419
ATCGCTA302.1661253E-645.00000410
AGCGTAT253.891609E-545.014
TCGCATA207.034256E-445.036
CGAACTA207.034256E-445.039
CGAACGC253.891609E-545.013
CGACGGT2550.045.028
CGACGAT502.1827873E-1145.036
CTATCGT453.8562575E-1045.013
CGTCTAG207.034256E-445.039
ATCCGAT207.034256E-445.044
GTAGCGA351.2123928E-745.034
TGACCTA207.034256E-445.031
CGTAGTC207.034256E-445.018