##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547884_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1711308
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444483401000873	31.0	31.0	33.0	30.0	34.0
2	31.837241455074132	31.0	31.0	34.0	30.0	34.0
3	31.887345235340455	33.0	31.0	34.0	30.0	34.0
4	35.654259782575664	37.0	35.0	37.0	33.0	37.0
5	35.57335967575679	37.0	35.0	37.0	33.0	37.0
6	35.612024837142116	37.0	35.0	37.0	33.0	37.0
7	35.972289032716496	37.0	35.0	37.0	35.0	37.0
8	35.99441888894343	37.0	35.0	37.0	35.0	37.0
9	37.77167348016839	39.0	38.0	39.0	35.0	39.0
10	37.27311623623567	39.0	37.0	39.0	34.0	39.0
11	36.95237268802577	39.0	37.0	39.0	33.0	39.0
12	35.99878397109112	37.0	35.0	39.0	32.0	39.0
13	35.576270314870264	37.0	35.0	39.0	31.0	39.0
14	36.47849072171696	38.0	35.0	41.0	31.0	41.0
15	36.799344127415985	38.0	35.0	41.0	32.0	41.0
16	36.99582015627812	38.0	35.0	41.0	32.0	41.0
17	36.91978416509477	38.0	35.0	41.0	32.0	41.0
18	36.85467840973104	38.0	35.0	40.0	32.0	41.0
19	36.73105192051928	38.0	35.0	40.0	32.0	41.0
20	36.556776454033994	37.0	35.0	40.0	32.0	41.0
21	36.42710546552696	37.0	35.0	40.0	31.0	41.0
22	36.31325278675726	37.0	35.0	40.0	31.0	41.0
23	36.260753178270654	37.0	35.0	40.0	31.0	41.0
24	36.25652717102941	37.0	35.0	40.0	31.0	41.0
25	36.18623006495616	37.0	35.0	40.0	31.0	41.0
26	36.05473006612486	36.0	35.0	40.0	31.0	41.0
27	35.97503546994463	36.0	35.0	40.0	31.0	41.0
28	35.959712687605034	36.0	35.0	40.0	31.0	41.0
29	35.98494426485472	36.0	35.0	40.0	31.0	41.0
30	35.916269309791105	36.0	35.0	40.0	31.0	41.0
31	35.70846218214372	36.0	35.0	40.0	30.0	41.0
32	35.484528208832074	36.0	34.0	40.0	30.0	41.0
33	35.32585309015093	36.0	34.0	40.0	29.0	41.0
34	35.2011706834772	36.0	34.0	40.0	29.0	41.0
35	35.0995875669371	36.0	34.0	40.0	28.0	41.0
36	34.904359121794556	36.0	34.0	40.0	27.0	41.0
37	34.761573603349014	36.0	34.0	40.0	26.0	41.0
38	34.64435916854242	35.0	34.0	40.0	26.0	41.0
39	34.55993836293642	36.0	34.0	40.0	25.0	41.0
40	34.323256246099476	36.0	34.0	40.0	23.0	41.0
41	34.29371919023343	36.0	34.0	40.0	23.0	41.0
42	34.20810982009083	35.0	34.0	40.0	23.0	41.0
43	34.10261332267482	35.0	33.0	40.0	23.0	41.0
44	33.89772676806279	35.0	33.0	40.0	23.0	41.0
45	33.75002571132724	35.0	33.0	40.0	23.0	41.0
46	33.69856215245882	35.0	33.0	40.0	23.0	41.0
47	33.6199807398785	35.0	33.0	40.0	23.0	41.0
48	33.53893396162468	35.0	33.0	39.0	23.0	41.0
49	33.44413980417318	35.0	33.0	39.0	22.0	41.0
50	33.322552690690394	35.0	33.0	39.0	21.0	41.0
51	32.95426714536483	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	7.0
14	21.0
15	52.0
16	163.0
17	429.0
18	892.0
19	1632.0
20	3026.0
21	4792.0
22	7486.0
23	10986.0
24	16168.0
25	22799.0
26	28527.0
27	32537.0
28	35290.0
29	40392.0
30	46536.0
31	54581.0
32	67909.0
33	89379.0
34	169364.0
35	285433.0
36	107405.0
37	130477.0
38	200062.0
39	354893.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.882810107823953	25.264008582908517	29.878256865508725	13.97492444375881
2	29.228812113307484	27.547934094856096	31.52436615734865	11.698887634487772
3	28.932489066842436	26.537245194903548	31.4107104039717	13.119555334282316
4	24.92695645669862	29.37951555184689	31.45944505606238	14.23408293539211
5	23.278977250150177	32.210332681200576	30.320257954734043	14.190432113915206
6	20.719648362539065	41.1215280942998	27.188326122474738	10.9704974206864
7	86.7529398565308	4.512630105159329	7.018783293247037	1.7156467450628408
8	87.25261612754687	3.7050606904192582	5.919740923317135	3.122582258716724
9	84.26758946957533	5.31125899019931	7.782351277502355	2.6388002627230165
10	50.889495052906895	24.160291426207323	13.087124001056502	11.863089519829277
11	44.44804792591398	21.86140659659161	20.387446327604383	13.303099149890027
12	40.681104745609794	21.419931420878065	23.042316169853702	14.856647663658443
13	20.716960360145574	41.720777323544326	23.440841742106038	14.12142057420406
14	13.860041558854396	43.38289776007592	27.592403004017978	15.164657677051704
15	12.446619778555352	25.477178859679263	48.01076135914751	14.06544000261788
16	15.021317027677075	20.511094437704962	46.19448982883268	18.27309870578528
17	15.263880026272304	21.794264971588984	27.659778368359174	35.28207663377954
18	20.521262098932514	25.239816561367096	33.18187024194359	21.057051097756805
19	25.85881676472032	27.613147370315573	25.40518714340142	21.122848721562686
20	28.642009503841503	26.08881627386771	25.32793629200588	19.941237930284906
21	20.36296213188976	29.566974501375554	28.96042091780089	21.109642448933798
22	20.89863426104477	25.040203166233084	27.00495761137095	27.0562049613512
23	18.18252471209157	31.123269452372103	25.20411287740138	25.490092958134948
24	19.65484880570885	25.85957641757065	36.6316875746505	17.853887202069995
25	16.5593803102656	27.407456752378884	33.93603021782169	22.09713271953383
26	15.258328716981397	35.58897638531462	26.90164482372548	22.2510500739785
27	16.624067672213304	34.09970618965142	29.08073824232692	20.195487895808352
28	13.575580783821497	30.601855422869523	37.59001886276462	18.232544930544357
29	14.060005562996258	25.978082262222813	37.03629036970551	22.925621805075416
30	17.687172618838922	30.224366391087987	32.56006516652759	19.528395823545498
31	27.372103677421013	27.823454340188906	24.736166721595414	20.068275260794664
32	27.39132873801794	28.47973596804316	25.41138123587338	18.717554058065527
33	25.696601663756613	28.378702138948686	25.89498792736316	20.029708269931536
34	17.127074728803933	28.592748938239055	29.642764481905072	24.637411851051944
35	18.10311177181431	26.743753900525213	31.491233606107144	23.661900721553337
36	28.152033415375843	23.698247188700105	28.98472980901159	19.16498958691247
37	19.931888356742327	29.56498771699776	31.60535683816122	18.897767088098693
38	19.565735682881165	31.08294941646974	26.48243332001019	22.868881580638902
39	20.125190789735104	29.036970551180737	29.24862152225082	21.589217136833348
40	22.556897998490044	25.740603094241365	27.944122273722787	23.7583766335458
41	16.572820322233053	23.08386333728353	30.57626096529672	29.7670553751867
42	22.918259015910635	24.718402531864513	26.0819793982147	26.28135905401015
43	22.091639845077566	25.733766218588354	27.627580774471927	24.547013161862154
44	19.327029383372253	30.10983411519142	27.28766534136462	23.27547116007171
45	17.839570667582922	34.65542146708833	23.239299997428866	24.265707867899874
46	22.90522804778567	29.611092801529594	27.59345483104152	19.89022431964322
47	21.13149707708957	26.343066239391156	28.812113307481763	23.713323376037508
48	22.427815448767845	23.48753117498428	31.610382233940353	22.474271142307522
49	21.046591262356046	21.65133336605684	31.802925014082795	25.49915035750432
50	18.860894707440156	28.385889623609543	29.681740516610688	23.071475152339612
51	17.359294761667684	30.839918939197386	26.67760566771148	25.12318063142345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2047.0
1	2266.0
2	2485.0
3	7880.5
4	13276.0
5	10110.0
6	6944.0
7	7243.5
8	7543.0
9	8504.5
10	9466.0
11	10092.5
12	10719.0
13	10849.5
14	10980.0
15	10665.5
16	10351.0
17	9925.0
18	9499.0
19	9098.5
20	8698.0
21	9038.5
22	9379.0
23	9651.5
24	9924.0
25	10027.0
26	11504.5
27	12879.0
28	15525.0
29	18171.0
30	21687.0
31	25203.0
32	28551.0
33	31899.0
34	36307.0
35	40715.0
36	41780.5
37	42846.0
38	52914.0
39	62982.0
40	87392.5
41	111803.0
42	138092.5
43	164382.0
44	172872.5
45	181363.0
46	180235.5
47	179108.0
48	172712.0
49	166316.0
50	149860.5
51	133405.0
52	119758.5
53	106112.0
54	92368.0
55	78624.0
56	69595.0
57	60566.0
58	56867.5
59	53169.0
60	45788.0
61	38407.0
62	33381.0
63	28355.0
64	24671.0
65	20987.0
66	17036.0
67	13085.0
68	10097.0
69	7109.0
70	5870.0
71	4631.0
72	3875.5
73	3120.0
74	2514.5
75	1560.5
76	1212.0
77	1025.0
78	838.0
79	571.5
80	305.0
81	269.5
82	234.0
83	144.0
84	54.0
85	49.5
86	45.0
87	30.5
88	16.0
89	14.5
90	13.0
91	8.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1711308.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.287151408266567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7762805502058	16.769226114523153
2	8.893605236447263	3.786390424672105
3	3.248090489254296	2.074277820975205
4	1.6324431991421862	1.3900026218213908
5	0.9660338674414222	1.0282054600869435
6	0.5542068681899273	0.7078491308796129
7	0.42503550538990365	0.6333456609986785
8	0.31551226991118203	0.537308596861216
9	0.2456933396671602	0.4707100189347755
>10	2.261348131856742	11.287802294348774
>50	1.4500012224645946	23.100608585659753
>100	1.2225861713461093	35.70808207744871
>500	0.006108765788967524	0.8774624588906429
>1k	0.002776711722257965	1.1145457813972355
>5k	2.776711722257965E-4	0.5141829525018323
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8699	0.5083246265429717	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4856	0.28375955701720557	No Hit
GAGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	2150	0.1256348944783756	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1792	0.10471522367686004	No Hit
AAGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1725	0.100800089755906	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12002515035282953	0.0
2	0.0	0.0	0.0	0.4065895794328081	0.0
3	0.0	0.0	0.0	0.6026384496537152	0.0
4	0.0	0.0	0.0	1.153854244823258	0.0
5	0.0	0.0	0.0	1.86155852716168	0.0
6	0.0	0.0	0.0	2.7686424652955517	0.0
7	0.0	0.0	0.0	3.1755242189015656	0.0
8	0.0	0.0	0.0	4.1113580956788605	0.0
9	0.0	0.0	0.0	4.448468656723395	0.0
10	0.0	0.0	0.0	5.120001776418973	0.0
11	0.0	0.0	0.0	5.949016775472328	0.0
12	0.0	0.0	0.0	6.6154660645541306	0.0
13	0.0	0.0	0.0	6.881052387997952	0.0
14	0.0	0.0	0.0	6.981501868746012	0.0
15	0.0	0.0	0.0	7.136587919883504	0.0
16	0.0	0.0	0.0	7.5199788699637935	0.0
17	0.0	0.0	0.0	7.9287889731129635	0.0
18	0.0	0.0	0.0	8.54305595486026	0.0
19	0.0	0.0	0.0	8.812382107721112	0.0
20	0.0	0.0	0.0	9.083519740455838	0.0
21	0.0	0.0	0.0	9.449263370474513	0.0
22	0.0	0.0	0.0	9.79174993630603	0.0
23	0.0	0.0	0.0	10.186477244306694	0.0
24	0.0	0.0	0.0	10.458900443403525	0.0
25	0.0	0.0	0.0	10.678556986819439	0.0
26	0.0	0.0	0.0	10.885124127275745	0.0
27	0.0	0.0	0.0	11.09589857582621	0.0
28	0.0	0.0	0.0	11.30421876132175	0.0
29	0.0	0.0	0.0	11.542749756326739	0.0
30	0.0	0.0	0.0	11.866069696395973	0.0
31	0.0	0.0	0.0	12.139778461854908	0.0
32	0.0	0.0	0.0	12.370888232860478	0.0
33	0.0	0.0	0.0	12.620989325124407	0.0
34	0.0	0.0	0.0	12.869337372349104	0.0
35	0.0	0.0	0.0	13.193066356260825	0.0
36	0.0	0.0	0.0	13.44398553620973	0.0
37	0.0	0.0	0.0	13.69946263326064	0.0
38	0.0	0.0	0.0	13.951375205398444	0.0
39	0.0	0.0	0.0	14.268676357499643	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGC	30	2.1661253E-6	45.000004	18
GCGAAAC	30	2.1661253E-6	45.000004	43
CGTTGCA	30	2.1661253E-6	45.000004	19
ATTACGA	30	2.1661253E-6	45.000004	30
CCATGCG	60	0.0	45.000004	1
TGCGCAC	30	2.1661253E-6	45.000004	13
CGTAACA	30	2.1661253E-6	45.000004	19
ATCGCTA	30	2.1661253E-6	45.000004	10
AGCGTAT	25	3.891609E-5	45.0	14
TCGCATA	20	7.034256E-4	45.0	36
CGAACTA	20	7.034256E-4	45.0	39
CGAACGC	25	3.891609E-5	45.0	13
CGACGGT	255	0.0	45.0	28
CGACGAT	50	2.1827873E-11	45.0	36
CTATCGT	45	3.8562575E-10	45.0	13
CGTCTAG	20	7.034256E-4	45.0	39
ATCCGAT	20	7.034256E-4	45.0	44
GTAGCGA	35	1.2123928E-7	45.0	34
TGACCTA	20	7.034256E-4	45.0	31
CGTAGTC	20	7.034256E-4	45.0	18
>>END_MODULE