FastQCFastQC Report
Thu 26 May 2016
SRR1547883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547883_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1969501
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87980.4467121367290497No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37350.18964194483780408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG650.045.0000041
ACTATCG207.034515E-445.014
CACGCAT406.8193913E-945.026
ATTCGTT207.034515E-445.014
ATTAACG207.034515E-445.01
ATATCGT207.034515E-445.035
TTGCCGA253.891823E-545.029
TAAGTCG302.1662945E-644.9999961
ACGTTAG1100.042.9545441
CGACGGT2450.042.244928
CGGTCTA2500.041.39999831
GCGATAA1850.041.3513539
CGTTTCG603.6379788E-1241.24999642
CGGGTAC2250.041.06
TAACGGG4100.040.6097563
GGCGATA5550.040.540548
CGTTTTT49300.040.0253521
TCGCTAA451.929584E-840.00000430
CCACGCA451.929584E-840.00000425
CGAATAT1300.039.80769314