##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547883_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1969501
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570484604983697	31.0	31.0	33.0	30.0	34.0
2	31.989941614652647	31.0	31.0	34.0	30.0	34.0
3	32.03969787270989	33.0	31.0	34.0	30.0	34.0
4	35.8108282250174	37.0	35.0	37.0	35.0	37.0
5	35.714407354959455	37.0	35.0	37.0	33.0	37.0
6	35.76944261515988	37.0	35.0	37.0	35.0	37.0
7	36.05361713449244	37.0	35.0	37.0	35.0	37.0
8	36.074797118661024	37.0	35.0	37.0	35.0	37.0
9	37.82969036319352	39.0	38.0	39.0	35.0	39.0
10	37.426991405437214	39.0	37.0	39.0	35.0	39.0
11	37.13967091156592	39.0	37.0	39.0	33.0	39.0
12	36.36180992038085	38.0	35.0	39.0	33.0	39.0
13	36.050115739976775	38.0	35.0	39.0	32.0	39.0
14	36.99243412417663	39.0	35.0	41.0	32.0	41.0
15	37.22688234227858	39.0	35.0	41.0	32.0	41.0
16	37.378453222415224	39.0	35.0	41.0	33.0	41.0
17	37.31377135629786	39.0	35.0	41.0	33.0	41.0
18	37.23615169527713	39.0	35.0	41.0	33.0	41.0
19	37.13804258032872	38.0	35.0	41.0	32.0	41.0
20	36.98683118211161	38.0	35.0	41.0	32.0	41.0
21	36.84809502508504	38.0	35.0	41.0	32.0	41.0
22	36.78175791736079	38.0	35.0	41.0	32.0	41.0
23	36.72410930484422	38.0	35.0	41.0	32.0	41.0
24	36.735338545144174	38.0	35.0	41.0	32.0	41.0
25	36.697099417568204	38.0	35.0	41.0	32.0	41.0
26	36.584951213530736	38.0	35.0	41.0	32.0	41.0
27	36.49499086316788	38.0	35.0	40.0	31.0	41.0
28	36.44502490732424	38.0	35.0	40.0	31.0	41.0
29	36.44255321525605	38.0	35.0	40.0	31.0	41.0
30	36.382329331135146	38.0	35.0	40.0	31.0	41.0
31	36.17967546094163	38.0	35.0	40.0	31.0	41.0
32	36.02867376051091	38.0	35.0	40.0	30.0	41.0
33	35.904040160426426	38.0	35.0	40.0	30.0	41.0
34	35.741496450116045	38.0	35.0	40.0	30.0	41.0
35	35.649462478059164	38.0	35.0	40.0	29.0	41.0
36	35.50971388184114	38.0	35.0	40.0	29.0	41.0
37	35.38705286262866	37.0	34.0	40.0	28.0	41.0
38	35.26497371669271	37.0	34.0	40.0	28.0	41.0
39	35.16228222275592	37.0	34.0	40.0	27.0	41.0
40	34.93639657964124	37.0	34.0	40.0	26.0	41.0
41	34.86966394025695	37.0	34.0	40.0	25.0	41.0
42	34.796121961857345	36.0	34.0	40.0	25.0	41.0
43	34.70352998043667	36.0	34.0	40.0	25.0	41.0
44	34.4839037908587	36.0	34.0	40.0	24.0	41.0
45	34.31279649007541	36.0	34.0	40.0	23.0	41.0
46	34.306861991946185	35.0	34.0	40.0	23.0	41.0
47	34.21504584156088	35.0	33.0	40.0	23.0	41.0
48	34.154308121701895	35.0	34.0	40.0	23.0	41.0
49	34.03368822864269	35.0	33.0	40.0	24.0	41.0
50	33.926535198509676	35.0	33.0	40.0	24.0	41.0
51	33.59187377919585	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	6.0
11	7.0
12	5.0
13	19.0
14	30.0
15	84.0
16	149.0
17	365.0
18	825.0
19	1593.0
20	2728.0
21	4489.0
22	6969.0
23	10397.0
24	15447.0
25	22032.0
26	28323.0
27	32435.0
28	35284.0
29	40195.0
30	46947.0
31	56309.0
32	70437.0
33	94818.0
34	176325.0
35	280807.0
36	132460.0
37	170337.0
38	264324.0
39	475272.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.98043463801237	23.954290959994434	29.577948932242226	12.487325469750967
2	31.59922234109046	26.094376189704903	29.00577354365395	13.300627925550684
3	29.40445320921391	25.90651134475179	30.61252571082726	14.07650973520704
4	25.60882172692474	28.418315096057327	30.378456268872167	15.59440690814577
5	23.817505043155602	32.16896056412259	29.254262881816256	14.759271510905556
6	21.8834110772221	40.44455930715446	26.945454711624922	10.726574903998525
7	86.99041026127938	4.655240083655707	6.471029971551169	1.8833196835137427
8	87.96187460681665	3.631681324355763	6.033152559963159	2.373291508864428
9	83.36304475092929	6.0129951698425135	7.681285767308572	2.9426743119196184
10	48.853745187232704	27.20882091453622	12.968056375701256	10.969377522529818
11	41.64024288385738	22.670158583316283	20.98364001846153	14.705958514364806
12	38.40851058212207	21.556018504179487	24.188817370491307	15.846653543207138
13	23.925501941862432	35.005465851502485	24.82745629476705	16.24157591186803
14	17.502098247220996	37.87126789983859	28.81496379032049	15.811670062619923
15	16.39466037336361	25.50854251914571	42.50228865078007	15.59450845671061
16	20.181558679076577	22.154494971061197	40.282995540494774	17.380950809367448
17	20.214257316954903	22.16063865923399	27.41024249289541	30.214861530915698
18	24.403389487997213	24.639946869790876	31.065940052835717	19.89072358937619
19	27.76804886110746	27.30356572553149	24.908542823791407	20.01984258956964
20	30.53311473312276	25.80633368553761	24.527786479925627	19.132765101414012
21	23.652996368115577	27.70046829120676	29.059391185889215	19.587144154788447
22	23.408619746829274	23.933676601332014	27.774547969257185	24.883155682581524
23	21.04020256907714	29.622884172183717	25.82984217829796	23.50707108044119
24	21.251068163966405	26.04878088409196	33.75570766402251	18.94444328791912
25	20.656095122571656	25.836798254989464	31.032581349285937	22.474525273152945
26	19.188109069251553	32.19125047410486	26.748348947271417	21.87229150937217
27	19.424107933938597	31.094069005296266	29.527834715493924	19.953988345271213
28	16.75043577027887	29.58185855198855	34.50361284406558	19.164092833667006
29	18.232181654134727	26.950836785561417	34.2585253828254	20.558456177478458
30	21.046244708685094	28.08675903185629	31.558653689437072	19.308342570021544
31	27.74068152288321	27.409023910117337	25.467770770362648	19.382523796636814
32	28.673709736628723	27.28107271841954	26.151903451686493	17.893314093265246
33	26.522149519091382	28.087368323245332	25.105851685274594	20.28463047238869
34	20.24294478652207	29.199782076779858	28.500975627836695	22.05629750886138
35	20.77368836065582	28.512704487075663	29.154135996884488	21.559471155384028
36	28.172009052039073	26.79201483015241	26.13733123263202	18.8986448851765
37	21.87010821522812	30.673353301166134	27.731389829200392	19.725148654405356
38	22.363786563195447	31.121994860627133	23.94474539489952	22.569473181277896
39	21.43218002935769	28.918137132197447	27.072491966239166	22.5771908722057
40	24.210954957626324	25.93230468022103	26.79211637871725	23.064623983435396
41	19.016491994672762	24.76007882199603	28.31707117691232	27.906358006418884
42	23.56764479936796	25.863302430412578	25.44334834051874	25.125704429700725
43	23.293362125736415	26.006790552530816	26.76276884347863	23.937078478254136
44	21.742157023530325	28.917680163655668	27.610496262758943	21.729666550055065
45	19.606844576367312	32.96459356964023	24.697524906054884	22.731036947937575
46	23.58506037823794	29.159365747973727	26.284170457389965	20.971403416398367
47	22.033449081772492	28.016385876422508	27.10884635245171	22.841318689353294
48	23.822023954290962	25.988308713730024	28.521945406476057	21.66772192550296
49	23.099556689740194	24.003135819682246	29.555557473695117	23.341750016882447
50	20.21080466575036	29.403183852153415	28.163428198310132	22.222583283786097
51	19.37500920283869	31.0694942526051	25.742967381077747	23.812529163478466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1578.0
1	2543.0
2	3508.0
3	9409.5
4	15311.0
5	11748.5
6	8186.0
7	7952.5
8	7719.0
9	8501.0
10	9283.0
11	9557.0
12	9831.0
13	9629.5
14	9428.0
15	9324.0
16	9220.0
17	8985.0
18	8750.0
19	8840.0
20	8930.0
21	9725.5
22	10521.0
23	10844.0
24	11167.0
25	12578.0
26	15866.0
27	17743.0
28	20469.0
29	23195.0
30	25858.0
31	28521.0
32	33085.0
33	37649.0
34	42743.5
35	47838.0
36	50794.0
37	53750.0
38	64925.0
39	76100.0
40	97321.5
41	118543.0
42	138962.0
43	159381.0
44	164509.5
45	169638.0
46	167109.0
47	164580.0
48	162505.5
49	160431.0
50	155252.5
51	150074.0
52	136827.0
53	123580.0
54	113943.0
55	104306.0
56	97035.0
57	89764.0
58	85813.5
59	81863.0
60	75805.5
61	69748.0
62	62126.5
63	54505.0
64	46058.5
65	37612.0
66	32618.0
67	27624.0
68	22969.0
69	18314.0
70	14629.5
71	10945.0
72	9094.5
73	7244.0
74	5821.5
75	3556.5
76	2714.0
77	1848.5
78	983.0
79	742.5
80	502.0
81	401.5
82	301.0
83	214.0
84	127.0
85	91.5
86	56.0
87	38.0
88	20.0
89	17.5
90	15.0
91	12.0
92	9.0
93	7.0
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1969501.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.70204709243856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3014487743151	15.624008782501605
2	7.8469434678711005	3.0920169947139917
3	2.741565181507088	1.6204333893912755
4	1.3279345166694423	1.0465211353238397
5	0.8139163135731944	0.8017908769661533
6	0.5293988558021765	0.6258144712618545
7	0.4141742958299527	0.5712057036643515
8	0.32324876922296064	0.5094929979042859
9	0.27334842932492315	0.4846971264483374
>10	3.2223593968253037	16.290492074883545
>50	2.19147195103765	31.670009277517547
>100	1.006884331188985	26.013992536203563
>500	0.00600112454064217	0.7931474559320226
>1k	0.00104367383315516	0.4041047476234124
>5k	2.6091845828879E-4	0.45227242966426434
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8798	0.4467121367290497	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3735	0.18964194483780408	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03894387461595602	0.0
2	0.0	0.0	0.0	0.14338657355340262	0.0
3	0.0	0.0	0.0	0.22828117375924156	0.0
4	0.0	0.0	0.0	0.4252346152654911	0.0
5	0.0	0.0	0.0	0.7375472264294357	0.0
6	0.0	0.0	0.0	1.1782679978329536	0.0
7	0.0	0.0	0.0	1.3766938935293762	0.0
8	0.0	0.0	0.0	1.874586506937544	0.0
9	0.0	0.0	0.0	2.093322115601871	0.0
10	0.0	0.0	0.0	2.496774563709285	0.0
11	0.0	0.0	0.0	2.9950733713768107	0.0
12	0.0	0.0	0.0	3.3876093487639762	0.0
13	0.0	0.0	0.0	3.561409717486815	0.0
14	0.0	0.0	0.0	3.6462535434102343	0.0
15	0.0	0.0	0.0	3.76151116450309	0.0
16	0.0	0.0	0.0	3.999388677639666	0.0
17	0.0	0.0	0.0	4.251635312701034	0.0
18	0.0	0.0	0.0	4.605887481143701	0.0
19	0.0	0.0	0.0	4.775625907272959	0.0
20	0.0	0.0	0.0	4.9632876550963925	0.0
21	0.0	0.0	0.0	5.1927874116337085	0.0
22	0.0	0.0	0.0	5.414214057266282	0.0
23	0.0	0.0	0.0	5.6560519644316	0.0
24	0.0	0.0	0.0	5.833914275748019	0.0
25	0.0	0.0035034254869634493	0.0	5.974762135180434	0.0
26	0.0	0.0035034254869634493	0.0	6.125561753967122	0.0
27	0.0	0.0035034254869634493	0.0	6.302560902482406	0.0
28	0.0	0.0035034254869634493	0.0	6.46463241196628	0.0
29	0.0	0.0035034254869634493	1.0154856483952026E-4	6.657828556573467	0.0
30	0.0	0.0035034254869634493	1.0154856483952026E-4	6.878595136534584	0.0
31	0.0	0.0035034254869634493	1.0154856483952026E-4	7.073923801003401	0.0
32	0.0	0.0035034254869634493	1.0154856483952026E-4	7.252700049403376	0.0
33	0.0	0.0035034254869634493	1.0154856483952026E-4	7.434471980466118	0.0
34	0.0	0.0035034254869634493	1.0154856483952026E-4	7.630460710606392	0.0
35	0.0	0.0035034254869634493	2.5387141209880064E-4	7.873567974832204	0.0
36	0.0	0.0035034254869634493	2.5387141209880064E-4	8.072450839070404	0.0
37	0.0	0.0035034254869634493	2.5387141209880064E-4	8.291084899169892	0.0
38	0.0	0.0035034254869634493	2.5387141209880064E-4	8.529724026542764	0.0
39	0.0	0.0035034254869634493	2.5387141209880064E-4	8.928860660644498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	65	0.0	45.000004	1
ACTATCG	20	7.034515E-4	45.0	14
CACGCAT	40	6.8193913E-9	45.0	26
ATTCGTT	20	7.034515E-4	45.0	14
ATTAACG	20	7.034515E-4	45.0	1
ATATCGT	20	7.034515E-4	45.0	35
TTGCCGA	25	3.891823E-5	45.0	29
TAAGTCG	30	2.1662945E-6	44.999996	1
ACGTTAG	110	0.0	42.954544	1
CGACGGT	245	0.0	42.2449	28
CGGTCTA	250	0.0	41.399998	31
GCGATAA	185	0.0	41.351353	9
CGTTTCG	60	3.6379788E-12	41.249996	42
CGGGTAC	225	0.0	41.0	6
TAACGGG	410	0.0	40.609756	3
GGCGATA	555	0.0	40.54054	8
CGTTTTT	4930	0.0	40.025352	1
TCGCTAA	45	1.929584E-8	40.000004	30
CCACGCA	45	1.929584E-8	40.000004	25
CGAATAT	130	0.0	39.807693	14
>>END_MODULE