FastQCFastQC Report
Thu 26 May 2016
SRR1547882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547882_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1769393
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416682.354931889071563No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43080.24347332672843172No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT41940.23703043925233117No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC32050.1811355645693184No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT27460.15519446499449246No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG18190.10280361683356948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.034319E-445.0000041
CGTTTTT163500.043.981651
CGGTCTA1550.042.09677531
CGACGGT1500.042.00000428
CGGTTTT14300.041.2237781
ATAGCGG2650.040.754722
TCACGAC1550.040.64516425
CACGACC5150.040.19417627
CCGCTCG451.929402E-840.00000419
AGGGCGC2250.040.06
CACGTGA4450.039.9438243
TTCGTAG1300.039.8076931
TACGGGA8400.039.6428574
GCGAGAC5400.039.58333621
CGTTAGG1950.039.230772
CGGGAGT3400.039.044126
GTAGCCG1100.038.86363643
ACGACCA5400.038.75000428
ACACGAC5350.038.6915926
TATCGTG356.2501986E-638.571431