##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547882_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1769393
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47354092618203	31.0	31.0	33.0	30.0	34.0
2	31.833470009206547	31.0	31.0	34.0	30.0	34.0
3	31.546413939695704	31.0	31.0	34.0	30.0	34.0
4	35.52062034833415	37.0	35.0	37.0	33.0	37.0
5	35.57038939342475	37.0	35.0	37.0	33.0	37.0
6	35.66205190141478	37.0	35.0	37.0	33.0	37.0
7	36.030833172732116	37.0	35.0	37.0	35.0	37.0
8	36.07254013099408	37.0	35.0	37.0	35.0	37.0
9	37.82460708276793	39.0	38.0	39.0	35.0	39.0
10	37.38687391664825	39.0	37.0	39.0	35.0	39.0
11	37.127609298782126	39.0	37.0	39.0	33.0	39.0
12	36.78884736177887	39.0	35.0	39.0	33.0	39.0
13	36.685015143611395	39.0	35.0	39.0	33.0	39.0
14	37.77666973928347	40.0	36.0	41.0	33.0	41.0
15	37.92616394435832	40.0	36.0	41.0	33.0	41.0
16	37.99932971363626	40.0	36.0	41.0	33.0	41.0
17	37.90874102022558	40.0	36.0	41.0	33.0	41.0
18	37.71956201929136	39.0	36.0	41.0	33.0	41.0
19	37.544936031735176	39.0	36.0	41.0	33.0	41.0
20	37.28517576366585	39.0	35.0	41.0	33.0	41.0
21	37.1878514270148	39.0	35.0	41.0	32.0	41.0
22	37.12379499636316	39.0	35.0	41.0	32.0	41.0
23	37.02448636340259	39.0	35.0	41.0	32.0	41.0
24	37.026953311107256	39.0	35.0	41.0	32.0	41.0
25	36.994890338099	39.0	35.0	41.0	32.0	41.0
26	36.89308706432093	38.0	35.0	41.0	32.0	41.0
27	36.83009710109625	38.0	35.0	41.0	32.0	41.0
28	36.73203183238546	38.0	35.0	41.0	32.0	41.0
29	36.70032152269168	38.0	35.0	41.0	31.0	41.0
30	36.56848930678487	38.0	35.0	40.0	31.0	41.0
31	36.3619619835729	38.0	35.0	40.0	31.0	41.0
32	36.07998336152568	38.0	35.0	40.0	30.0	41.0
33	35.83808515123548	38.0	35.0	41.0	29.0	41.0
34	35.5313539728031	38.0	35.0	40.0	27.0	41.0
35	35.287075850305726	38.0	35.0	40.0	25.0	41.0
36	35.12879332064725	38.0	35.0	40.0	24.0	41.0
37	34.98443929641408	38.0	34.0	40.0	24.0	41.0
38	34.817462259656274	38.0	34.0	40.0	23.0	41.0
39	34.685871369447035	38.0	34.0	40.0	23.0	41.0
40	34.47711220740672	38.0	34.0	40.0	21.0	41.0
41	34.324398254090525	38.0	34.0	40.0	21.0	41.0
42	34.174740716166504	38.0	33.0	40.0	20.0	41.0
43	34.04824818454691	37.0	33.0	40.0	18.0	41.0
44	33.79868406849128	37.0	33.0	40.0	18.0	41.0
45	33.64087006108875	37.0	33.0	40.0	18.0	41.0
46	33.57755682315913	37.0	33.0	40.0	18.0	41.0
47	33.462968373899976	36.0	33.0	40.0	17.0	41.0
48	33.331576421970695	36.0	33.0	40.0	17.0	41.0
49	33.18285310273071	36.0	33.0	40.0	15.0	41.0
50	33.099470835478606	36.0	32.0	40.0	15.0	41.0
51	32.78095143362724	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	10.0
14	11.0
15	57.0
16	104.0
17	273.0
18	636.0
19	1309.0
20	2317.0
21	4034.0
22	6430.0
23	10040.0
24	16616.0
25	27189.0
26	37606.0
27	41338.0
28	40519.0
29	40661.0
30	44251.0
31	50738.0
32	60828.0
33	77212.0
34	120940.0
35	177125.0
36	135393.0
37	167730.0
38	251315.0
39	454635.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.179780862702632	23.377678107690038	27.574428066574242	19.868112963033084
2	36.53524118158035	24.30731895062318	26.671632588124854	12.485807279671617
3	27.17824700335087	24.11030223359084	36.19179006585874	12.519660697199548
4	23.96991510648002	26.373055618508722	35.78119728064935	13.87583199436191
5	21.77786393412882	29.932016233815776	34.38597304273273	13.904146789322667
6	19.808601028714367	38.04570267882828	31.34792553152409	10.797770760933268
7	81.85349439044916	2.9017860927448	13.138630027359666	2.106089489446381
8	82.09515918735974	2.3100577429660905	12.841239905436497	2.7535431642376795
9	77.11576794979973	4.843525434993809	14.82672306265482	3.213983552551638
10	35.19172959314296	35.837939903684486	18.99832315375951	9.972007349413047
11	26.47631136779675	23.462226876674656	34.71574715170683	15.345714603821762
12	25.816932699518986	21.905873935298715	34.59282364064965	17.68436972453265
13	20.824881753234017	27.100819320524046	36.00285521644994	16.071443709792003
14	15.81830605184942	30.474066530160343	36.376542690063765	17.33108472792647
15	15.029447951924757	26.921605318886193	42.365093565985624	15.683853163203427
16	19.249313182543393	25.59442701536629	39.44233983066509	15.713919971425227
17	17.954405832960795	25.836374395060908	34.62464246213249	21.584577309845805
18	20.028450434697096	26.705825104993636	35.491097794554406	17.774626665754866
19	21.342403863923955	29.15655255785459	31.766487151243396	17.734556426978067
20	21.906100001525946	29.07115604051785	31.531999957047418	17.490744000908787
21	19.991658156215152	28.81796186601846	34.36319687033915	16.82718310742724
22	19.52036658899408	24.853155856273876	34.16064153073964	21.465836023992406
23	16.398561540596127	29.828590934857317	33.626220969564145	20.146626554982415
24	17.17340353443243	28.465298551537167	36.9376390660526	17.423658847977812
25	18.990071736465556	28.7037984212665	33.71167400345768	18.59445583881026
26	17.243710131101455	31.175323967032764	32.0040827560638	19.57688314580198
27	17.66374118129777	29.081781153197735	34.12695766288213	19.12752000262237
28	16.269647274517308	29.287840519319335	36.41649989572695	18.026012310436403
29	17.10106234171832	27.940259738791777	35.40140601890027	19.557271900589637
30	18.95797033219867	27.292184381875593	35.8380529367981	17.91179234912764
31	21.412823493706597	27.707863657197695	32.402524481559496	18.476788367536212
32	21.196930246700422	28.513563691051115	32.52663484030964	17.762871221938823
33	20.553771830226523	27.728322650762156	32.01352102105072	19.7043844979606
34	16.993511334112885	29.30609536716829	33.82662868000495	19.873764618713878
35	17.826113249006863	28.75986284561994	33.68109854622461	19.732925359148588
36	20.738072321977086	28.787273375671766	30.261790342789872	20.212863959561272
37	18.38986590316566	29.768683384640948	31.401107611480324	20.44034310071307
38	18.303112988465536	30.142540407925207	29.912404988603434	21.641941615005823
39	17.86443147452262	28.562676578917177	29.68701696005353	23.88587498650667
40	20.145326674175834	27.218882407695748	31.03013293259327	21.605657985535153
41	17.47497588155938	27.223516765353995	31.38601769081261	23.915489662274013
42	19.652276232583716	29.025886278514722	29.87035667033836	21.4514808185632
43	19.641538086790217	28.48666181001055	30.78434242703571	21.08745767616352
44	20.210885880072997	30.0902625928779	29.736977596271714	19.96187393077739
45	18.748746038895824	30.49147362965718	28.755624103859347	22.004156227587654
46	20.265424357392618	29.22719825386446	29.637169356948966	20.870208031793954
47	19.56721881458783	29.075055682937595	30.621970359326617	20.735755143147962
48	19.681382259339784	28.55719447290681	30.46847139103636	21.29295187671704
49	20.14267039600586	27.09607192975218	30.47050598708145	22.290751687160512
50	17.6432256711765	28.980107867500323	32.09710900856961	21.279557452753572
51	17.33142382726732	29.760714550131034	30.26026439575606	22.64759722684559
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1373.0
1	1887.5
2	2402.0
3	29548.0
4	56694.0
5	38922.0
6	21150.0
7	18832.5
8	16515.0
9	17748.5
10	18982.0
11	20176.5
12	21371.0
13	22079.0
14	22787.0
15	21883.0
16	20979.0
17	19510.5
18	18042.0
19	17129.0
20	16216.0
21	16331.5
22	16447.0
23	16575.5
24	16704.0
25	17554.5
26	21138.5
27	23872.0
28	26770.5
29	29669.0
30	33169.5
31	36670.0
32	41284.5
33	45899.0
34	50821.0
35	55743.0
36	59030.5
37	62318.0
38	66895.0
39	71472.0
40	82986.5
41	94501.0
42	105177.0
43	115853.0
44	121431.5
45	127010.0
46	140443.0
47	153876.0
48	155602.0
49	157328.0
50	147571.5
51	137815.0
52	121207.0
53	104599.0
54	90429.0
55	76259.0
56	67854.5
57	59450.0
58	53258.5
59	47067.0
60	40501.5
61	33936.0
62	28843.0
63	23750.0
64	19689.5
65	15629.0
66	12749.5
67	9870.0
68	8099.0
69	6328.0
70	5456.5
71	4585.0
72	3991.0
73	3397.0
74	2828.0
75	1660.5
76	1062.0
77	801.0
78	540.0
79	416.5
80	293.0
81	201.0
82	109.0
83	96.5
84	84.0
85	69.0
86	54.0
87	35.0
88	16.0
89	11.0
90	6.0
91	5.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1769393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.968108905028323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8365595356974	22.127420573383873
2	8.720031349116468	5.226456982511667
3	3.639293951796013	3.2718827245450144
4	2.0955637738357855	2.5120033356697187
5	1.4128657397826576	2.1170457178995044
6	1.0684680829620443	1.9211980723052031
7	0.7919172456995104	1.66125835840251
8	0.6628618432280199	1.5891772725476088
9	0.568446070598719	1.5331728375304043
>10	6.721840444388382	43.900628560798694
>50	0.4395394875082136	8.0679390090121
>100	0.038790744164345116	2.0192277364167386
>500	0.0015286924890068952	0.3270978441690022
>1k	0.0021019521723844815	1.3393717572249286
>5k	0.0	0.0
>10k+	1.910865611258619E-4	2.3861192175829804
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41668	2.354931889071563	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4308	0.24347332672843172	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT	4194	0.23703043925233117	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTC	3205	0.1811355645693184	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT	2746	0.15519446499449246	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTG	1819	0.10280361683356948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08601819946162328	0.0
2	0.0	0.0	0.0	0.2814524529033403	0.0
3	0.0	0.0	0.0	0.5443109586168816	0.0
4	0.0	0.0	0.0	0.8077911464553098	0.0
5	0.0	0.0	0.0	1.5129482257474738	0.0
6	0.0	0.0	0.0	2.865672012944552	0.0
7	0.0	0.0	0.0	3.7862136902316217	0.0
8	0.0	0.0	0.0	5.320186075111635	0.0
9	0.0	0.0	0.0	6.330758627393688	0.0
10	0.0	0.0	0.0	7.437748425590018	0.0
11	0.0	0.0	0.0	8.249891346919537	0.0
12	0.0	0.0	0.0	8.81596117990746	0.0
13	0.0	0.0	0.0	9.15794286515206	0.0
14	0.0	0.0	0.0	9.374796893623971	0.0
15	0.0	0.0	0.0	9.54355533225236	0.0
16	0.0	0.0	0.0	9.78250733443616	0.0
17	0.0	0.0	0.0	10.074923999360232	0.0
18	0.0	0.0	0.0	10.470596413572338	0.0
19	0.0	0.0	0.0	10.687902574498713	0.0
20	0.0	0.0	0.0	10.917303278581977	0.0
21	0.0	0.0	0.0	11.155746631754505	0.0
22	0.0	0.0	0.0	11.420809283183555	0.0
23	0.0	0.0	0.0	11.70994798781277	0.0
24	0.0	0.0	0.0	11.928554029545726	0.0
25	0.0	0.0	0.0	12.114323951773292	0.0
26	0.0	0.0	0.0	12.30354138396614	0.0
27	0.0	0.0	0.0	12.529098962186467	0.0
28	0.0	0.0	0.0	12.742561997249904	0.0
29	0.0	0.0	0.0	12.982361747785822	0.0
30	0.0	0.0	0.0	13.300154346716642	0.0
31	0.0	0.0	0.0	13.529272468015868	0.0
32	0.0	0.0	0.0	13.779414748447632	0.0
33	0.0	0.0	0.0	14.025035704334764	0.0
34	0.0	0.0	0.0	14.270713176778703	0.0
35	0.0	0.0	0.0	14.523511735380438	0.0
36	0.0	0.0	0.0	14.750934359975426	0.0
37	0.0	0.0	0.0	14.995877117180864	0.0
38	0.0	0.0	0.0	15.278855517118018	0.0
39	0.0	0.0	0.0	15.735509296125846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.034319E-4	45.000004	1
CGTTTTT	16350	0.0	43.98165	1
CGGTCTA	155	0.0	42.096775	31
CGACGGT	150	0.0	42.000004	28
CGGTTTT	1430	0.0	41.223778	1
ATAGCGG	265	0.0	40.75472	2
TCACGAC	155	0.0	40.645164	25
CACGACC	515	0.0	40.194176	27
CCGCTCG	45	1.929402E-8	40.000004	19
AGGGCGC	225	0.0	40.0	6
CACGTGA	445	0.0	39.94382	43
TTCGTAG	130	0.0	39.807693	1
TACGGGA	840	0.0	39.642857	4
GCGAGAC	540	0.0	39.583336	21
CGTTAGG	195	0.0	39.23077	2
CGGGAGT	340	0.0	39.04412	6
GTAGCCG	110	0.0	38.863636	43
ACGACCA	540	0.0	38.750004	28
ACACGAC	535	0.0	38.69159	26
TATCGTG	35	6.2501986E-6	38.57143	1
>>END_MODULE