FastQCFastQC Report
Thu 26 May 2016
SRR1547881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547881_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2531188
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110720.4374230598438362No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC43500.17185606126451294No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34590.13665519906067822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT4700.042.60638428
CGGTCTA4650.042.58064331
CGTTTTT62400.040.7812541
ACGGGAT8700.040.3448265
AACGTCG403.4604273E-739.3750041
TACGGGA6450.039.0697674
TACGTTG1000.038.2500041
TAAGGGA26400.038.0113644
TAGGGTA12150.037.9629635
CGGGTAT1900.037.8947376
GTTGATC11400.037.6973716
TCACGAC5150.037.57281525
TAATGCG1500.037.51
CGAGGTA301.14030976E-437.49999619
ACGCTAG900.037.4999961
GTACGTC301.14030976E-437.49999644
GACCGAT10500.036.8571439
CGAATAT5800.036.85344714
ATTAGCG1600.036.5625041
CACGACG5300.036.50943426
Produced by FastQC (version 0.11.5)