FastQCFastQC Report
Thu 26 May 2016
SRR1547880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547880_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2618379
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191610.7317886371682633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100100.043.021981
TATAGCG2050.041.7073171
TAACGCG501.0822987E-940.51
CGGTCTA4600.040.108731
TACGGGA10050.039.8507464
CGTTAGG4750.039.7894742
GGGCGAT38100.039.2716527
GACCGAT10300.037.791269
CGGGATA5850.037.692316
AATGCGG4850.037.577322
ATAGGGA35650.037.5525934
ACGGGAT11750.037.3404245
GGCGATA7850.037.2611478
GTAGGGA36750.037.102044
AGGGCGA19950.036.9924856
TAAGGGA37350.036.867474
GCGATAT2150.036.6279079
TAGGGAT43950.036.55295
TAGGGAG31650.036.5402835
TAATACG1050.036.428571
Produced by FastQC (version 0.11.5)