##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547880_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2618379
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51469325105342	31.0	31.0	33.0	30.0	34.0
2	31.936540508459622	31.0	31.0	34.0	30.0	34.0
3	31.943648341206526	33.0	31.0	34.0	30.0	34.0
4	35.746864758692304	37.0	35.0	37.0	35.0	37.0
5	35.700585362164915	37.0	35.0	37.0	33.0	37.0
6	35.750628537732695	37.0	35.0	37.0	33.0	37.0
7	36.04678237947982	37.0	35.0	37.0	35.0	37.0
8	36.073725003141256	37.0	35.0	37.0	35.0	37.0
9	37.83104164828698	39.0	38.0	39.0	35.0	39.0
10	37.381156814960704	39.0	37.0	39.0	35.0	39.0
11	37.112884345619946	39.0	37.0	39.0	33.0	39.0
12	36.60398704694775	39.0	35.0	39.0	33.0	39.0
13	36.44235154651027	39.0	35.0	39.0	32.0	39.0
14	37.50434753715944	40.0	35.0	41.0	33.0	41.0
15	37.69101149986308	40.0	35.0	41.0	33.0	41.0
16	37.7667289571143	40.0	35.0	41.0	33.0	41.0
17	37.70292001272543	40.0	35.0	41.0	33.0	41.0
18	37.5946973299129	39.0	36.0	41.0	33.0	41.0
19	37.49448952959064	39.0	36.0	41.0	33.0	41.0
20	37.334411481301984	39.0	35.0	41.0	32.0	41.0
21	37.226616925968315	39.0	35.0	41.0	32.0	41.0
22	37.15061341387171	39.0	35.0	41.0	32.0	41.0
23	37.05037811562039	39.0	35.0	41.0	32.0	41.0
24	37.07533477773844	39.0	35.0	41.0	32.0	41.0
25	37.03767598197205	39.0	35.0	41.0	32.0	41.0
26	36.926797839426605	39.0	35.0	41.0	32.0	41.0
27	36.85659753610917	39.0	35.0	41.0	32.0	41.0
28	36.78327125293932	39.0	35.0	41.0	31.0	41.0
29	36.78080904254121	38.0	35.0	41.0	31.0	41.0
30	36.68746579467678	38.0	35.0	41.0	31.0	41.0
31	36.51523442557399	38.0	35.0	41.0	31.0	41.0
32	36.35778051993237	38.0	35.0	41.0	30.0	41.0
33	36.2342143746188	38.0	35.0	41.0	30.0	41.0
34	36.05219870767372	38.0	35.0	41.0	30.0	41.0
35	35.92676155743687	38.0	35.0	41.0	29.0	41.0
36	35.811832053342926	38.0	35.0	41.0	29.0	41.0
37	35.708984451830695	38.0	35.0	40.0	28.0	41.0
38	35.61186902278089	38.0	35.0	40.0	28.0	41.0
39	35.527823512180625	38.0	35.0	40.0	27.0	41.0
40	35.38436299710622	38.0	35.0	40.0	27.0	41.0
41	35.322323468069364	38.0	34.0	40.0	26.0	41.0
42	35.23937061823365	38.0	34.0	40.0	26.0	41.0
43	35.183439830521095	38.0	34.0	40.0	26.0	41.0
44	34.96604005760816	38.0	34.0	40.0	25.0	41.0
45	34.85174873461787	37.0	34.0	40.0	24.0	41.0
46	34.82893003648441	37.0	34.0	40.0	25.0	41.0
47	34.764353823491554	37.0	34.0	40.0	24.0	41.0
48	34.65870983535997	37.0	34.0	40.0	24.0	41.0
49	34.519355295776506	37.0	34.0	40.0	24.0	41.0
50	34.45919784721769	37.0	34.0	40.0	24.0	41.0
51	34.12966763023993	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	5.0
12	3.0
13	14.0
14	28.0
15	86.0
16	193.0
17	449.0
18	968.0
19	1840.0
20	3232.0
21	5402.0
22	8366.0
23	12378.0
24	18779.0
25	27872.0
26	37326.0
27	42965.0
28	45989.0
29	50101.0
30	57863.0
31	69724.0
32	86897.0
33	113758.0
34	198066.0
35	287824.0
36	186375.0
37	247539.0
38	392053.0
39	722184.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.95903992508342	25.23267258101291	29.650291267994433	14.157996225909237
2	31.725812038669726	26.174591226098286	28.989959054819796	13.109637680412195
3	28.710664116997574	24.929316955261253	32.29792172943642	14.062097198304752
4	25.546492696435468	27.40497078535995	31.731388007618456	15.31714851058613
5	23.232732923690573	31.946101003712602	30.22163712739829	14.599528945198536
6	22.44117448238013	40.172412015220104	26.850620173779273	10.535793328620494
7	88.64759456136792	3.105470980327905	6.778545046381749	1.4683894119224146
8	89.30464229968236	2.4547248507568993	6.5288867654377	1.7117460841230396
9	84.2354372686307	5.288615590027265	7.927805714909873	2.548141426432155
10	42.08649702735929	34.89628506797526	13.141107532561177	9.876110372104268
11	33.70073621885907	21.91661329395019	27.70569119290981	16.676959294280927
12	31.36016596527852	20.97545848022765	29.944328151119453	17.720047403374377
13	23.877406593927006	28.391268032626293	30.259675929267686	17.471649444179015
14	18.465241280960473	31.66566031884613	31.224700473078954	18.644397927114447
15	17.13636566746067	26.00666290097805	39.78228514664989	17.07468628491139
16	21.526639191652546	23.293877624285866	37.02493030993603	18.154552874125557
17	21.759836906727408	23.074543448446537	29.264327280351697	25.901292364474354
18	23.13801783469849	24.952575620259708	31.98585842614839	19.923548118893407
19	25.72209752675224	26.16718206187874	27.946145305931648	20.164575105437372
20	27.009497097249863	26.46855172608702	27.42551784902033	19.09643332764279
21	24.517191743441266	25.806615467050413	31.00681757682902	18.669375212679295
22	23.836121508765537	23.217876403683345	29.65334659344579	23.292655494105322
23	20.828420942881074	26.787947810458302	29.521394725515286	22.862236521145334
24	20.63360575378889	25.33697375360863	34.017344318755995	20.01207617384649
25	20.818949433981864	26.63014025089569	31.313954167826736	21.236956147295714
26	20.147350708205344	30.031710459028275	28.01725800581199	21.803680826954384
27	19.597124785984	29.38016230652629	30.698688005059623	20.32402490243009
28	18.116093965006595	27.459775685643674	34.30622534018185	20.117905009167885
29	19.300032577407624	26.09561106318069	33.22154661338179	21.382809746029892
30	21.107066624044876	26.866393291421907	31.58289919068248	20.443640893850738
31	25.19302973328155	25.982678596184893	28.179572170415362	20.644719500118203
32	25.175347037231816	26.619828527497358	28.453138373016284	19.75168606225455
33	24.479764006662137	26.532713560565526	28.33684504802399	20.65067738474835
34	20.856644511737986	26.94697750020146	30.0527540130745	22.143623974986053
35	21.14182095105407	26.567658845415426	30.385020655909628	21.905499547620874
36	25.328571608617395	25.776520511354544	28.58982599539639	20.305081884631676
37	21.378608673534274	28.26473936737195	29.890096124357857	20.46655583473592
38	21.02132655356616	28.553658580365944	27.898023930072767	22.526990935995133
39	20.974350924751537	27.36754304858082	28.90181291554813	22.756293111119515
40	23.164904698670437	25.57395243392954	28.342039101291295	22.919103766108726
41	20.011579683460646	24.916026289547847	29.670036308723834	25.402357718267677
42	21.63537058615273	25.99871905480452	28.48995504470514	23.87595531433761
43	21.746240708468868	25.863673669854514	29.352702569032214	23.037383052644405
44	20.95174151641149	27.925674625407552	29.311150142893755	21.811433715287208
45	20.21426233559007	29.698679984830314	27.205725374363297	22.881332305216322
46	22.659171953334486	27.970320568565516	28.081496223426782	21.289011254673216
47	21.856958064512433	26.676352048347468	29.30202235810782	22.164667529032275
48	21.79226154807994	25.90862514555761	30.38051405086888	21.91859925549357
49	21.654046262974152	24.75760766489496	30.49657822645232	23.091767845678568
50	20.51406614550453	27.34737790060186	29.734274526338623	22.404281427554988
51	19.79629381384437	28.07362876038954	28.256413605517	23.873663820249096
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1252.0
1	2245.5
2	3239.0
3	17302.0
4	31365.0
5	23041.5
6	14718.0
7	13555.5
8	12393.0
9	12719.0
10	13045.0
11	13242.0
12	13439.0
13	13343.0
14	13247.0
15	12608.0
16	11969.0
17	11488.0
18	11007.0
19	11062.5
20	11118.0
21	11478.0
22	11838.0
23	12710.0
24	13582.0
25	17044.5
26	22726.5
27	24946.0
28	31160.0
29	37374.0
30	44024.0
31	50674.0
32	57437.0
33	64200.0
34	71750.0
35	79300.0
36	85871.5
37	92443.0
38	103393.5
39	114344.0
40	133749.5
41	153155.0
42	170054.0
43	186953.0
44	194696.0
45	202439.0
46	205887.5
47	209336.0
48	207524.0
49	205712.0
50	198414.5
51	191117.0
52	177995.0
53	164873.0
54	149406.0
55	133939.0
56	125705.5
57	117472.0
58	110940.0
59	104408.0
60	96817.5
61	89227.0
62	77766.5
63	66306.0
64	58924.0
65	51542.0
66	43313.0
67	35084.0
68	29020.0
69	22956.0
70	19055.0
71	15154.0
72	12322.0
73	9490.0
74	7925.5
75	4827.5
76	3294.0
77	2531.5
78	1769.0
79	1372.0
80	975.0
81	650.0
82	325.0
83	298.5
84	272.0
85	198.0
86	124.0
87	90.5
88	57.0
89	41.5
90	26.0
91	18.5
92	11.0
93	6.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2618379.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.395587853647275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.022965487257	15.709286593437923
2	8.104676897686963	3.305992993843998
3	2.869963942888757	1.7560380520196275
4	1.4415014084221538	1.1760107446652128
5	0.8704486583657691	0.8876656041894223
6	0.6370579026651774	0.7795902253000739
7	0.45562764053875765	0.6504955499810777
8	0.3641348631462553	0.5941395673500223
9	0.31440083483438347	0.5771150863312243
>10	4.893883994420991	26.271247293071465
>50	2.7150543806306144	38.89113493222292
>100	0.30551630160510146	7.8741907988186215
>500	0.0038141500305598685	0.525094866238108
>1k	7.628300061119738E-4	0.25671304571640835
>5k	0.0	0.0
>10k+	1.9070750152799345E-4	0.7452846468139546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19161	0.7317886371682633	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04346200454556044	0.0
2	0.0	0.0	0.0	0.1433711468049507	0.0
3	0.0	0.0	0.0	0.23231930900759593	0.0
4	0.0	0.0	0.0	0.35239359924594565	0.0
5	0.0	0.0	0.0	0.635584077018644	0.0
6	0.0	0.0	0.0	1.0359844774190443	0.0
7	3.8191568141968754E-5	0.0	0.0	1.257571955778747	0.0
8	3.8191568141968754E-5	0.0	0.0	1.7088817165123917	0.0
9	3.8191568141968754E-5	0.0	0.0	1.9521620055767328	0.0
10	3.8191568141968754E-5	0.0	0.0	2.3595514629471133	0.0
11	3.8191568141968754E-5	0.0	0.0	2.830759030682724	0.0
12	3.8191568141968754E-5	0.0	0.0	3.199956919911136	0.0
13	3.8191568141968754E-5	0.0	0.0	3.357267989087905	0.0
14	3.8191568141968754E-5	0.0	0.0	3.4324671867594416	0.0
15	3.8191568141968754E-5	0.0	0.0	3.5344004821303563	0.0
16	3.8191568141968754E-5	0.0	0.0	3.7410168657784073	0.0
17	3.8191568141968754E-5	0.0	0.0	4.000337613462375	0.0
18	3.8191568141968754E-5	0.0	0.0	4.309078250322051	0.0
19	3.8191568141968754E-5	0.0	0.0	4.473645717445794	0.0
20	3.8191568141968754E-5	0.0	0.0	4.659524079592756	0.0
21	3.8191568141968754E-5	0.0	0.0	4.876757719184274	0.0
22	3.8191568141968754E-5	0.0	0.0	5.1243154638805155	0.0
23	3.8191568141968754E-5	0.0	0.0	5.3853166405627295	0.0
24	3.8191568141968754E-5	0.0	0.0	5.57310458111679	0.0
25	3.8191568141968754E-5	0.0	0.0	5.73217246242809	0.0
26	3.8191568141968754E-5	0.0	0.0	5.89357002939605	0.0
27	3.8191568141968754E-5	0.0	0.0	6.07360508161729	0.0
28	3.8191568141968754E-5	0.0	0.0	6.248560655275649	0.0
29	3.8191568141968754E-5	0.0	0.0	6.44371956848111	0.0
30	3.8191568141968754E-5	0.0	0.0	6.68505208757021	0.0
31	3.8191568141968754E-5	0.0	0.0	6.896747949781143	0.0
32	3.8191568141968754E-5	0.0	0.0	7.096260701754788	0.0
33	3.8191568141968754E-5	0.0	0.0	7.294322174139038	0.0
34	3.8191568141968754E-5	0.0	0.0	7.503841116965878	0.0
35	3.8191568141968754E-5	0.0	0.0	7.741010755127505	0.0
36	3.8191568141968754E-5	0.0	0.0	7.95392874751898	0.0
37	3.8191568141968754E-5	0.0	0.0	8.182352516576096	0.0
38	3.8191568141968754E-5	0.0	0.0	8.419980453555425	0.0
39	3.8191568141968754E-5	0.0	0.0	8.744226867080739	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10010	0.0	43.02198	1
TATAGCG	205	0.0	41.707317	1
TAACGCG	50	1.0822987E-9	40.5	1
CGGTCTA	460	0.0	40.1087	31
TACGGGA	1005	0.0	39.850746	4
CGTTAGG	475	0.0	39.789474	2
GGGCGAT	3810	0.0	39.271652	7
GACCGAT	1030	0.0	37.79126	9
CGGGATA	585	0.0	37.69231	6
AATGCGG	485	0.0	37.57732	2
ATAGGGA	3565	0.0	37.552593	4
ACGGGAT	1175	0.0	37.340424	5
GGCGATA	785	0.0	37.261147	8
GTAGGGA	3675	0.0	37.10204	4
AGGGCGA	1995	0.0	36.992485	6
TAAGGGA	3735	0.0	36.86747	4
GCGATAT	215	0.0	36.627907	9
TAGGGAT	4395	0.0	36.5529	5
TAGGGAG	3165	0.0	36.540283	5
TAATACG	105	0.0	36.42857	1
>>END_MODULE