FastQCFastQC Report
Thu 26 May 2016
SRR1547879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547879_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1182841
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246172.0811757455144013No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25170.21279275912823448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110050.043.875511
CGGTCTA800.042.187531
TCGATTG451.9283107E-840.0000041
CGAGGGA7900.039.5886084
TAGCGCG403.4583354E-739.3751
CGTTATG403.4583354E-739.37531
CGGGATT6100.039.0983626
ACATACG750.039.017
ACGTAGG1850.038.918922
CGGTTTT10650.038.8732381
TAGGGAC12650.038.5968365
TTTAGCG700.038.571431
CACGAGG1050.038.571432
TACGGGA4900.038.571434
ATGGGAC10800.038.5416645
ACGGGAT6550.038.473285
GACCGAT4400.038.3522729
TAGGGAT20600.038.2281535
AGGGATT20950.038.0190936
GCGCGAC1600.037.968759
Produced by FastQC (version 0.11.5)