##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547879_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1182841
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.510120971457702	31.0	31.0	33.0	30.0	34.0
2	31.872880632308146	31.0	31.0	34.0	30.0	34.0
3	31.588275178151587	31.0	31.0	34.0	30.0	34.0
4	35.563350441859896	37.0	35.0	37.0	33.0	37.0
5	35.598539448666386	37.0	35.0	37.0	33.0	37.0
6	35.70790410545458	37.0	35.0	37.0	33.0	37.0
7	36.059729921434915	37.0	35.0	37.0	35.0	37.0
8	36.11268970216623	37.0	35.0	37.0	35.0	37.0
9	37.88026201323762	39.0	38.0	39.0	35.0	39.0
10	37.462118746306565	39.0	37.0	39.0	35.0	39.0
11	37.164477727775754	39.0	37.0	39.0	33.0	39.0
12	36.81475870383255	39.0	35.0	39.0	33.0	39.0
13	36.71776341875197	39.0	35.0	39.0	33.0	39.0
14	37.80779242518648	40.0	36.0	41.0	33.0	41.0
15	37.95019026225841	40.0	36.0	41.0	33.0	41.0
16	38.022843306919526	40.0	36.0	41.0	33.0	41.0
17	37.95940451844331	40.0	36.0	41.0	33.0	41.0
18	37.75849924038818	39.0	36.0	41.0	33.0	41.0
19	37.56336312319238	39.0	36.0	41.0	33.0	41.0
20	37.30486853262611	39.0	35.0	41.0	33.0	41.0
21	37.20413394530626	39.0	35.0	41.0	33.0	41.0
22	37.137676999698186	39.0	35.0	41.0	32.0	41.0
23	37.03951503202882	39.0	35.0	41.0	32.0	41.0
24	37.03727466328949	39.0	35.0	41.0	32.0	41.0
25	36.98364530820288	39.0	35.0	41.0	32.0	41.0
26	36.896232883371475	38.0	35.0	41.0	32.0	41.0
27	36.82428661164096	38.0	35.0	41.0	32.0	41.0
28	36.74263996598021	38.0	35.0	41.0	32.0	41.0
29	36.71395394647294	38.0	35.0	41.0	32.0	41.0
30	36.57289948522244	38.0	35.0	41.0	31.0	41.0
31	36.342015537168564	38.0	35.0	41.0	31.0	41.0
32	36.054260040022285	38.0	35.0	41.0	30.0	41.0
33	35.741608550937954	38.0	35.0	41.0	29.0	41.0
34	35.41176793837887	38.0	35.0	41.0	25.0	41.0
35	35.15038792196077	38.0	34.0	41.0	24.0	41.0
36	34.96783084116969	38.0	34.0	40.0	23.0	41.0
37	34.82190505739994	38.0	34.0	40.0	23.0	41.0
38	34.668275786855546	38.0	34.0	40.0	22.0	41.0
39	34.564971961573875	38.0	34.0	40.0	21.0	41.0
40	34.430359617226664	38.0	34.0	40.0	21.0	41.0
41	34.332244147776414	38.0	34.0	40.0	20.0	41.0
42	34.255730060084154	38.0	34.0	40.0	19.0	41.0
43	34.19492391623219	38.0	33.0	40.0	19.0	41.0
44	33.93817089532744	37.0	33.0	40.0	18.0	41.0
45	33.82391885299884	37.0	33.0	40.0	18.0	41.0
46	33.82666224792681	37.0	33.0	40.0	18.0	41.0
47	33.776342720619255	37.0	33.0	40.0	18.0	41.0
48	33.68021568410293	37.0	33.0	40.0	18.0	41.0
49	33.54864516870822	37.0	33.0	40.0	18.0	41.0
50	33.482922894962215	36.0	33.0	40.0	18.0	41.0
51	33.1636661224966	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	6.0
14	10.0
15	32.0
16	76.0
17	205.0
18	428.0
19	962.0
20	1730.0
21	2854.0
22	4343.0
23	7068.0
24	11527.0
25	18762.0
26	25806.0
27	28666.0
28	27772.0
29	26877.0
30	28340.0
31	31901.0
32	38056.0
33	48796.0
34	79570.0
35	115417.0
36	83363.0
37	106914.0
38	170059.0
39	323249.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.796904233113324	23.845470354849045	27.686138711796428	18.6714867002412
2	35.94912587575168	24.69630322249567	27.26224403787153	12.092326863881112
3	27.269683752930447	23.992911980562052	36.1710491942704	12.566355072237098
4	23.503919799871664	26.235309733091768	36.52545016616773	13.735320300868839
5	21.91241257278028	30.254784878102804	34.79157384635805	13.041228702758866
6	20.434191915904165	38.24047357168039	31.38088720292922	9.944447309486229
7	83.0579934243064	2.775182801407797	12.726731657086624	1.440092117199184
8	83.75225410684952	2.1994503065078064	12.348912491197042	1.6993830954456262
9	79.11029462117055	4.891274482369144	13.746818042323525	2.2516128541367775
10	38.660310219209514	35.11444057147157	17.915002946296248	8.310246263022671
11	26.734531521988163	23.492168431767247	34.15590091990386	15.617399126340734
12	26.940983614873005	21.865068931496285	34.68598061785143	16.507966835779282
13	22.65308693222504	25.866367499942932	35.16525044363528	16.315295124196744
14	17.331746194120765	29.03965959921917	36.223465368549114	17.405128838110954
15	16.205982038160666	26.21561139662896	41.70416818490397	15.8742383803064
16	20.9687523513304	24.876293601591424	38.57551437598122	15.579439671096962
17	20.33181129162753	24.259219962784517	34.283982378020376	21.124986367567576
18	21.009501699721262	25.230694573488744	36.35628119079403	17.403522535995965
19	22.73771369102018	26.60492830397323	33.11882154913467	17.538536455871924
20	23.725843118390383	27.37088078617498	32.39801461058587	16.505261484848766
21	21.961531600612425	26.04339890145844	35.61323964928506	16.38182984864407
22	21.080432619430674	23.65212230553388	35.392246295148716	19.87519877988673
23	18.59159430557446	26.972179692790494	34.92785589948269	19.508370102152362
24	18.798891820625087	26.41479285888805	37.33578731207322	17.45052800841364
25	19.61928949030343	27.152085529669666	34.679978120474345	18.54864685955255
26	18.11688975948585	29.713630149783445	32.86739299703003	19.302087093700678
27	17.472001731424598	28.502731981728736	35.72677984615007	18.298486440696593
28	16.76277707654706	27.85327867397224	37.28125758238005	18.102686667100652
29	18.081804739605744	26.64559311014752	36.55766075068416	18.71494139956258
30	19.399733353848912	27.486788165104187	35.28081965369817	17.83265882734873
31	22.054274412199103	27.01656435649424	32.819457560229985	18.109703671076673
32	23.022705503106504	27.723759998173886	32.245923162961034	17.00761133575857
33	22.05275265230069	28.213005805514012	31.62758139090546	18.106660151279844
34	19.089209792355863	28.82137159601333	33.118229753618614	18.97118885801219
35	19.39745071400129	28.961035337801107	32.294788564143445	19.346725384054153
36	22.641251021904043	29.035009777307348	30.46005337995555	17.863685820833062
37	20.375604159815225	30.32216502471592	30.91598955396372	18.38624126150514
38	20.38718644348649	30.537071339258613	29.59205844234348	19.48368377491142
39	19.62233301010026	30.065410312966833	29.92574657117905	20.386510105753857
40	21.231932271539456	28.628361715564477	30.234917457206844	19.904788555689226
41	18.883095868337332	27.943485219061564	31.061063997612525	22.112354914988575
42	20.596428429518422	28.872942348126244	29.860310895547247	20.670318326808083
43	20.153596299079926	28.661333180030113	30.469014854912874	20.716055665977084
44	20.20499796675969	30.00859794342604	29.930734561957184	19.855669527857085
45	19.017348908264086	30.47510189450653	29.068826663938772	21.438722533290612
46	20.453974794583548	29.807556552402225	29.854562024819902	19.88390662819432
47	19.791501985473957	28.967037835178182	30.879382774185203	20.362077405162655
48	20.37222247115208	28.293912706779693	31.05117255827284	20.282692263795386
49	20.82604508974579	26.905729510559745	31.379872696330274	20.888352703364188
50	19.074583988887774	28.934319997362284	31.354510031356707	20.63658598239324
51	18.346675504146372	29.86605976627459	30.181402234112614	21.605862495466425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	636.0
1	1125.0
2	1614.0
3	19556.0
4	37498.0
5	26569.0
6	15640.0
7	13914.0
8	12188.0
9	12402.5
10	12617.0
11	12785.0
12	12953.0
13	12712.5
14	12472.0
15	11962.0
16	11452.0
17	10972.0
18	10492.0
19	9990.5
20	9489.0
21	9375.5
22	9262.0
23	9798.0
24	10334.0
25	11175.0
26	13494.5
27	14973.0
28	18065.0
29	21157.0
30	23359.5
31	25562.0
32	29697.5
33	33833.0
34	36981.5
35	40130.0
36	43158.5
37	46187.0
38	49904.0
39	53621.0
40	59855.0
41	66089.0
42	72386.0
43	78683.0
44	82849.5
45	87016.0
46	88113.0
47	89210.0
48	89732.0
49	90254.0
50	85603.5
51	80953.0
52	73227.0
53	65501.0
54	59272.5
55	53044.0
56	48585.5
57	44127.0
58	40865.5
59	37604.0
60	33201.0
61	28798.0
62	25123.5
63	21449.0
64	17628.0
65	13807.0
66	11286.0
67	8765.0
68	7083.0
69	5401.0
70	4392.0
71	3383.0
72	2700.0
73	2017.0
74	1639.0
75	1008.0
76	755.0
77	514.0
78	273.0
79	231.5
80	190.0
81	130.5
82	71.0
83	47.5
84	24.0
85	20.0
86	16.0
87	12.0
88	8.0
89	7.0
90	6.0
91	6.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1182841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.02826910287851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1219580598052	23.09941480965958
2	7.58132039997959	4.856331398513777
3	3.494775828570433	3.3579486207506712
4	2.168379135617004	2.7779772189043404
5	1.6236654377611868	2.6001596786829166
6	1.3146148126279507	2.526290219128693
7	1.124041942258824	2.520078246671148
8	0.9847309506561902	2.5231382305239873
9	0.8938924116476827	2.576684403834573
>10	8.626501161476929	48.36545180522093
>50	0.04612529187943078	0.965533306292642
>100	0.01732862538843679	1.0729544321079343
>500	0.002132753864949064	0.44118697355208575
>1k	2.66594233118633E-4	0.21491534980270943
>5k	0.0	0.0
>10k+	2.66594233118633E-4	2.1019353063541115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24617	2.0811757455144013	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2517	0.21279275912823448	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.058587756088941796	0.0
2	0.0	0.0	0.0	0.17787682368128938	0.0
3	0.0	0.0	0.0	0.30764912612937834	0.0
4	0.0	0.0	0.0	0.4365760064116817	0.0
5	0.0	0.0	0.0	0.7645152645199144	0.0
6	0.0	0.0	0.0	1.284027185395163	0.0
7	0.0	0.0	0.0	1.6114591902039244	0.0
8	0.0	0.0	0.0	2.2329290242729156	0.0
9	0.0	0.0	0.0	2.636618108435538	0.0
10	0.0	0.0	0.0	3.1553691493615794	0.0
11	0.0	0.0	0.0	3.643431365669604	0.0
12	0.0	0.0	0.0	4.002482159478746	0.0
13	0.0	0.0	0.0	4.189151373684206	0.0
14	0.0	0.0	0.0	4.31511927638626	0.0
15	0.0	0.0	0.0	4.428744015467844	0.0
16	0.0	0.0	0.0	4.6169349895717176	0.0
17	0.0	0.0	0.0	4.834884823911244	0.0
18	0.0	0.0	0.0	5.1239346623933395	0.0
19	0.0	0.0	0.0	5.2778860387828965	0.0
20	0.0	0.0	0.0	5.456523742413393	0.0
21	0.0	0.0	0.0	5.648265489613566	0.0
22	0.0	0.0	0.0	5.878220318707248	0.0
23	0.0	0.0	0.0	6.1156994050764215	0.0
24	0.0	0.0	0.0	6.3048203435626595	0.0
25	0.0	0.0	0.0	6.4635906262971945	0.0
26	0.0	0.0	0.0	6.628532490841964	0.0
27	0.0	0.0	0.0	6.8207814913416085	0.0
28	0.0	0.0	0.0	7.004069016883926	0.0
29	0.0	0.0	0.0	7.214494593947961	0.0
30	0.0	0.0	0.0	7.47750542972386	0.0
31	0.0	0.0	0.0	7.6846338603413304	0.0
32	0.0	0.0	0.0	7.887366095696716	0.0
33	0.0	0.0	0.0	8.08553305135686	0.0
34	0.0	0.0	0.0	8.312951613953185	0.0
35	0.0	0.0	0.0	8.562520237293093	0.0
36	0.0	0.0	0.0	8.775059369771592	0.0
37	0.0	0.0	0.0	8.99529184395874	0.0
38	0.0	0.0	0.0	9.25711908870254	0.0
39	0.0	0.0	0.0	9.65387571110572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11005	0.0	43.87551	1
CGGTCTA	80	0.0	42.1875	31
TCGATTG	45	1.9283107E-8	40.000004	1
CGAGGGA	790	0.0	39.588608	4
TAGCGCG	40	3.4583354E-7	39.375	1
CGTTATG	40	3.4583354E-7	39.375	31
CGGGATT	610	0.0	39.098362	6
ACATACG	75	0.0	39.0	17
ACGTAGG	185	0.0	38.91892	2
CGGTTTT	1065	0.0	38.873238	1
TAGGGAC	1265	0.0	38.596836	5
TTTAGCG	70	0.0	38.57143	1
CACGAGG	105	0.0	38.57143	2
TACGGGA	490	0.0	38.57143	4
ATGGGAC	1080	0.0	38.541664	5
ACGGGAT	655	0.0	38.47328	5
GACCGAT	440	0.0	38.352272	9
TAGGGAT	2060	0.0	38.228153	5
AGGGATT	2095	0.0	38.019093	6
GCGCGAC	160	0.0	37.96875	9
>>END_MODULE