FastQCFastQC Report
Thu 26 May 2016
SRR1547878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547878_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1637464
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259901.5872104669171352No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25610.15640038498556302No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.15432400345900735No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22710.1386900719649409No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18190.11108641167072986No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16600.10137627453183702No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG207.034164E-445.0000041
GGCGTAA207.034164E-445.00000432
TCGATTG650.045.0000041
TACGAGA207.034164E-445.00000435
TTGACGT207.034164E-445.00000417
ACTACGC207.034164E-445.00000423
CGTACGT207.034164E-445.00000422
TTAACGA207.034164E-445.00000432
CATCGTA207.034164E-445.00000421
ATGCGAA207.034164E-445.00000413
ACCGTTG406.8175723E-945.00000411
ACCGTAC207.034164E-445.00000420
CTTTACG207.034164E-445.0000041
ATCGGAT207.034164E-445.00000435
CGTCCGC207.034164E-445.00000428
TTACCGG406.8175723E-945.0000042
GTGCGTA207.034164E-445.00000416
TCGCGAG406.8175723E-945.0000041
AACGTGC351.2123564E-745.045
AACGTCG253.8915343E-545.035