##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547878_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1637464
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426406320993927	31.0	31.0	33.0	30.0	34.0
2	31.8052506803203	31.0	31.0	34.0	30.0	34.0
3	31.77964767469697	31.0	31.0	34.0	30.0	34.0
4	35.60069167932852	37.0	35.0	37.0	33.0	37.0
5	35.50960326456032	37.0	35.0	37.0	33.0	37.0
6	35.58972227786382	37.0	35.0	37.0	33.0	37.0
7	35.98342742191584	37.0	35.0	37.0	35.0	37.0
8	36.012688523228604	37.0	35.0	37.0	35.0	37.0
9	37.794112725531676	39.0	38.0	39.0	35.0	39.0
10	37.240870028287645	39.0	37.0	39.0	34.0	39.0
11	36.95153481236839	39.0	37.0	39.0	33.0	39.0
12	36.4268735068374	38.0	35.0	39.0	32.0	39.0
13	36.251752099588145	39.0	35.0	39.0	32.0	39.0
14	37.342345846992664	39.0	35.0	41.0	32.0	41.0
15	37.510817337052906	40.0	35.0	41.0	33.0	41.0
16	37.60886773693956	40.0	35.0	41.0	33.0	41.0
17	37.54363210427832	39.0	35.0	41.0	33.0	41.0
18	37.447035782160704	39.0	36.0	41.0	33.0	41.0
19	37.34714900602395	39.0	35.0	41.0	32.0	41.0
20	37.16984068046687	39.0	35.0	41.0	32.0	41.0
21	37.041563051157155	39.0	35.0	41.0	32.0	41.0
22	36.93306784149148	39.0	35.0	41.0	32.0	41.0
23	36.827281699017504	38.0	35.0	41.0	32.0	41.0
24	36.824659351289554	38.0	35.0	41.0	32.0	41.0
25	36.762537680217704	38.0	35.0	41.0	32.0	41.0
26	36.6231911052701	38.0	35.0	41.0	31.0	41.0
27	36.522482326329005	38.0	35.0	40.0	31.0	41.0
28	36.447946336530144	38.0	35.0	40.0	31.0	41.0
29	36.39980909503964	38.0	35.0	40.0	31.0	41.0
30	36.314252404938365	38.0	35.0	40.0	31.0	41.0
31	36.18110138604574	38.0	35.0	40.0	30.0	41.0
32	36.004620559597036	38.0	35.0	40.0	30.0	41.0
33	35.89611008242013	38.0	35.0	40.0	30.0	41.0
34	35.74178730036202	38.0	35.0	40.0	30.0	41.0
35	35.66502225392436	38.0	35.0	40.0	29.0	41.0
36	35.54218657631557	38.0	35.0	40.0	29.0	41.0
37	35.44847276031717	38.0	35.0	40.0	28.0	41.0
38	35.33878118847193	38.0	34.0	40.0	27.0	41.0
39	35.276363938382765	37.0	34.0	40.0	27.0	41.0
40	35.11772961115481	37.0	34.0	40.0	26.0	41.0
41	35.058315785873766	37.0	34.0	40.0	26.0	41.0
42	34.97452646287186	37.0	34.0	40.0	26.0	41.0
43	34.88821433631518	37.0	34.0	40.0	26.0	41.0
44	34.66742352808978	36.0	34.0	40.0	24.0	41.0
45	34.5158837079777	36.0	34.0	40.0	24.0	41.0
46	34.46492686251423	36.0	34.0	40.0	24.0	41.0
47	34.3860616172325	36.0	33.0	40.0	24.0	41.0
48	34.29234169422961	36.0	33.0	40.0	23.0	41.0
49	34.14169227537216	36.0	33.0	40.0	24.0	41.0
50	34.04405837319172	35.0	33.0	40.0	23.0	41.0
51	33.713466677740705	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	0.0
13	10.0
14	24.0
15	50.0
16	123.0
17	270.0
18	573.0
19	1252.0
20	2173.0
21	3493.0
22	5521.0
23	8188.0
24	12275.0
25	17796.0
26	23414.0
27	26634.0
28	29600.0
29	33932.0
30	40304.0
31	48209.0
32	59474.0
33	77727.0
34	130383.0
35	208887.0
36	140275.0
37	150048.0
38	220127.0
39	396634.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.81983603914346	24.102331410034054	28.94720128198238	14.130631268840107
2	31.543655310895385	26.410046266666015	29.175908600128004	12.870389822310596
3	28.81205327262157	25.277441213974782	32.52859299502157	13.381912518382084
4	25.56025659190065	27.5591402314799	32.21029592100956	14.670307255609893
5	22.595305912068905	31.479104273437464	31.188105509495173	14.73748430499846
6	19.758785536659126	41.123163623749896	28.18010044800985	10.937950391581127
7	87.0784334800643	3.571559435810497	7.533722878793061	1.8162842053321477
8	86.95006424568723	3.0903274820087647	7.354115876745992	2.605492395558009
9	83.24604388249146	4.922734179194169	8.977479810243157	2.8537421280712123
10	43.3024481759599	26.90599610128833	16.083468094565742	13.708087628186025
11	33.18540132790705	27.78992393115207	24.094331234152325	14.930343506788546
12	32.484866842874105	25.441292144437984	26.928775228035544	15.145065784652365
13	21.037409066703148	35.05274009077451	26.732862524000527	17.176988318521815
14	15.210960363098058	38.68970554467152	28.289537968468316	17.80979612376211
15	13.920306034208995	30.847823219319633	38.574893860261966	16.656976886209407
16	15.739521601696282	27.828825549752544	39.236282446514856	17.19537040203632
17	15.851707274175189	28.790006986413136	28.310484993868567	27.04780074554311
18	18.826612371325417	31.743659707938615	31.004590024574586	18.42513789616138
19	23.20392997952932	33.97888442127583	24.466797437989477	18.350388161205373
20	24.913463746378547	31.45705798722903	25.14711773816096	18.48236052823146
21	20.006668848902937	34.1766902967027	27.66082185623623	18.15581899815813
22	20.12795395807175	29.562237704157162	27.254889267794592	23.054919069976503
23	17.545301759305854	34.5142244348578	26.886270476786056	21.054203329050285
24	18.55454532130172	31.539930038156566	32.59925103696936	17.306273603572354
25	17.699808972899554	32.54612009790749	30.140448889258025	19.613622039934924
26	16.227837680706266	38.06288260383129	25.591402314798984	20.117877400663463
27	16.782659038610927	36.98817195370402	28.1076103047151	18.12155870296996
28	15.712895062120449	34.00190782820264	32.82264526120879	17.46255184846812
29	15.80370621888481	31.995024012741652	32.08937723211014	20.111892536263394
30	18.34367045626652	34.679418906308776	28.45155679758456	18.525353839840143
31	23.306466584914233	33.74962747272612	23.977015677901925	18.96689026445772
32	22.792928577361092	34.22456921190328	24.463499655564945	18.51900255517068
33	22.347361529780198	33.96972391454102	23.69291782903319	19.989996726645593
34	17.750985670524667	33.444643668502025	26.90666787178222	21.89770278919109
35	18.164429874488842	31.291313885373967	28.478549757429782	22.065706482707405
36	23.319779854702148	31.97627550895775	25.99452568117528	18.709418955164818
37	17.781154272704622	35.0532897211786	28.066937654812563	19.09861835130421
38	18.527063801097306	36.026501956684235	25.857606640512405	19.58882760170605
39	19.330073821470272	33.417772848746594	26.865445591475602	20.38670773830753
40	21.147213007431002	30.6523380055989	27.72726606508601	20.473182921884085
41	17.235432351489866	28.79318262874787	29.359424085048584	24.61196093471368
42	20.391226921630032	31.34102490192151	25.235180742904884	23.032567433543576
43	19.808862973476057	32.83241646839259	25.54700439215763	21.811716165973724
44	18.783374779537137	34.81749827782473	26.186957392651074	20.212169549987053
45	18.36095327897285	36.89815470752334	23.75099544173185	20.98989657177196
46	21.043516071193018	34.99948701162285	25.22174533302717	18.735251584156963
47	19.21123151409741	32.53109686686242	27.136413380691117	21.121258238349057
48	19.464061499978015	31.014116951578785	29.239543586912447	20.282277961530756
49	19.531055339231884	30.129761631400754	28.20019249278152	22.138990536585844
50	17.083490079781907	32.16730260940088	29.64852967759902	21.100677633218197
51	16.877012257979413	33.87934024809095	26.70422067294304	22.5394268209866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1039.0
1	1866.0
2	2693.0
3	16806.0
4	30919.0
5	20591.5
6	10264.0
7	10195.0
8	10126.0
9	13115.0
10	16104.0
11	18854.5
12	21605.0
13	21922.5
14	22240.0
15	20255.0
16	18270.0
17	16281.0
18	14292.0
19	12777.0
20	11262.0
21	10857.5
22	10453.0
23	11113.0
24	11773.0
25	12357.5
26	16021.5
27	19101.0
28	22354.5
29	25608.0
30	28821.0
31	32034.0
32	38501.5
33	44969.0
34	49611.5
35	54254.0
36	56717.0
37	59180.0
38	62945.5
39	66711.0
40	83589.5
41	100468.0
42	115883.0
43	131298.0
44	135225.0
45	139152.0
46	143596.0
47	148040.0
48	151231.0
49	154422.0
50	139229.5
51	124037.0
52	106753.0
53	89469.0
54	76927.5
55	64386.0
56	58645.0
57	52904.0
58	48071.0
59	43238.0
60	38078.0
61	32918.0
62	27528.0
63	22138.0
64	17743.5
65	13349.0
66	10439.5
67	7530.0
68	6371.5
69	5213.0
70	4764.5
71	4316.0
72	4304.5
73	4293.0
74	3331.5
75	1548.0
76	726.0
77	662.5
78	599.0
79	434.5
80	270.0
81	221.0
82	172.0
83	146.5
84	121.0
85	106.5
86	92.0
87	75.5
88	59.0
89	44.5
90	30.0
91	18.5
92	7.0
93	6.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1637464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.614634794808147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20916742917551	17.76037022061559
2	9.561767543503448	4.515952970653675
3	3.971873414158679	2.813830203796949
4	2.1102831514715614	1.9933426374255085
5	1.3311298701165142	1.5717072873630944
6	0.913876334282669	1.294851352902194
7	0.6656178610439114	1.1002825891017327
8	0.4898815773522975	0.9254699633503249
9	0.3715502493150309	0.7896621100944933
>10	3.380066069875446	18.000693392125147
>50	1.2507343911656512	21.504088944392837
>100	0.7367318488700624	24.40437638926834
>500	0.0049673190612736905	0.7957975659089142
>1k	0.002091502762641554	0.9250143467814788
>5k	0.0	0.0
>10k+	2.6143784533019424E-4	1.604560026219758
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25990	1.5872104669171352	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2561	0.15640038498556302	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2527	0.15432400345900735	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2271	0.1386900719649409	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1819	0.11108641167072986	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1660	0.10137627453183702	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2214008979739401E-4	0.0	0.0	0.1020480450257227	0.0
2	1.2214008979739401E-4	0.0	0.0	0.35866437369004756	0.0
3	1.2214008979739401E-4	0.0	0.0	0.5610505024843294	0.0
4	1.2214008979739401E-4	0.0	0.0	0.8358046344835672	0.0
5	1.2214008979739401E-4	0.0	0.0	1.5365833996961153	0.0
6	1.2214008979739401E-4	0.0	0.0	2.338127738991514	0.0
7	1.2214008979739401E-4	0.0	0.0	2.953286301256089	0.0
8	1.2214008979739401E-4	0.0	0.0	4.162045699935999	0.0
9	1.2214008979739401E-4	0.0	0.0	4.731157448346956	0.0
10	1.2214008979739401E-4	0.0	0.0	5.508762330042065	0.0
11	1.2214008979739401E-4	0.0	0.0	6.427927575812354	0.0
12	1.2214008979739401E-4	0.0	0.0	7.004612009790749	0.0
13	1.2214008979739401E-4	0.0	0.0	7.2441287258834395	0.0
14	1.2214008979739401E-4	0.0	0.0	7.345688210549972	0.0
15	1.2214008979739401E-4	0.0	0.0	7.483645441976129	0.0
16	1.2214008979739401E-4	0.0	0.0	7.837973842478369	0.0
17	1.8321013469609103E-4	0.0	0.0	8.24805919397312	0.0
18	1.8321013469609103E-4	0.0	0.0	8.734970661950431	0.0
19	1.8321013469609103E-4	0.0	0.0	8.986579246933063	0.0
20	1.8321013469609103E-4	0.0	0.0	9.237454991376909	0.0
21	1.8321013469609103E-4	0.0	0.0	9.575660900025893	0.0
22	1.8321013469609103E-4	0.0	0.0	9.892919783274625	0.0
23	1.8321013469609103E-4	0.0	0.0	10.2642867263036	0.0
24	1.8321013469609103E-4	0.0	0.0	10.546308193645784	0.0
25	1.8321013469609103E-4	0.0	0.0	10.792298334497735	0.0
26	1.8321013469609103E-4	0.0	0.0	11.008303083304426	0.0
27	1.8321013469609103E-4	0.0	0.0	11.227849894715243	0.0
28	1.8321013469609103E-4	0.0	0.0	11.455152601828193	0.0
29	1.8321013469609103E-4	0.0	0.0	11.70511229559856	0.0
30	1.8321013469609103E-4	0.0	0.0	12.028905673651451	0.0
31	1.8321013469609103E-4	0.0	0.0	12.311537841442622	0.0
32	1.8321013469609103E-4	0.0	0.0	12.558505103012951	0.0
33	1.8321013469609103E-4	0.0	0.0	12.818968844505894	0.0
34	1.8321013469609103E-4	0.0	0.0	13.079188305819242	0.0
35	1.8321013469609103E-4	0.0	0.0	13.397668589965948	0.0
36	1.8321013469609103E-4	0.0	0.0	13.677674745826472	0.0
37	1.8321013469609103E-4	0.0	0.0	13.95994049334825	0.0
38	1.8321013469609103E-4	0.0	0.0	14.242145170825129	0.0
39	1.8321013469609103E-4	0.0	0.0	14.566121759012717	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	7.034164E-4	45.000004	1
GGCGTAA	20	7.034164E-4	45.000004	32
TCGATTG	65	0.0	45.000004	1
TACGAGA	20	7.034164E-4	45.000004	35
TTGACGT	20	7.034164E-4	45.000004	17
ACTACGC	20	7.034164E-4	45.000004	23
CGTACGT	20	7.034164E-4	45.000004	22
TTAACGA	20	7.034164E-4	45.000004	32
CATCGTA	20	7.034164E-4	45.000004	21
ATGCGAA	20	7.034164E-4	45.000004	13
ACCGTTG	40	6.8175723E-9	45.000004	11
ACCGTAC	20	7.034164E-4	45.000004	20
CTTTACG	20	7.034164E-4	45.000004	1
ATCGGAT	20	7.034164E-4	45.000004	35
CGTCCGC	20	7.034164E-4	45.000004	28
TTACCGG	40	6.8175723E-9	45.000004	2
GTGCGTA	20	7.034164E-4	45.000004	16
TCGCGAG	40	6.8175723E-9	45.000004	1
AACGTGC	35	1.2123564E-7	45.0	45
AACGTCG	25	3.8915343E-5	45.0	35
>>END_MODULE