FastQCFastQC Report
Thu 26 May 2016
SRR1547877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547877_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1476325
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85940.5821211454117488No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38860.26322117419944796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG253.891347E-545.01
TCACGAC2000.045.025
AAGTACG253.891347E-545.01
AATCCGT502.1827873E-1145.033
AATCCGG502.1827873E-1145.02
GTCGACG453.8562575E-1045.01
CGAGTAC207.0339395E-445.018
ACGTTGC253.891347E-545.011
CGCGGAT700.045.023
GACCGTA207.0339395E-445.09
TAGCGCC207.0339395E-445.019
GACCGAC351.2122473E-745.09
CAACCGG253.891347E-545.02
CCAGCGA207.0339395E-445.021
AAGCGGT207.0339395E-445.014
CGCGAGC700.045.017
AGGCGAT900.045.06
CCGCTCG453.8562575E-1045.019
AACGGTC253.891347E-545.017
TGTTGCG502.1827873E-1145.01
Produced by FastQC (version 0.11.5)