##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547877_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1476325 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.476945794455826 31.0 31.0 33.0 30.0 34.0 2 31.87039506883647 31.0 31.0 34.0 30.0 34.0 3 31.900769139586473 33.0 31.0 34.0 30.0 34.0 4 35.67590875992752 37.0 35.0 37.0 33.0 37.0 5 35.59939918378406 37.0 35.0 37.0 33.0 37.0 6 35.65166003420656 37.0 35.0 37.0 33.0 37.0 7 36.02696967131221 37.0 35.0 37.0 35.0 37.0 8 36.06402790713427 37.0 35.0 37.0 35.0 37.0 9 37.8472511134066 39.0 38.0 39.0 35.0 39.0 10 37.34459959697221 39.0 37.0 39.0 34.0 39.0 11 36.958351311533704 39.0 37.0 39.0 33.0 39.0 12 35.99483582544494 37.0 35.0 39.0 32.0 39.0 13 35.611416862818146 37.0 35.0 39.0 31.0 39.0 14 36.54297664809578 38.0 35.0 41.0 31.0 41.0 15 36.85797639408667 38.0 35.0 41.0 32.0 41.0 16 37.05231165224459 38.0 35.0 41.0 33.0 41.0 17 36.99530794371158 38.0 35.0 41.0 32.0 41.0 18 36.92271755880311 38.0 35.0 41.0 32.0 41.0 19 36.83083196450646 38.0 35.0 41.0 32.0 41.0 20 36.66928623439961 38.0 35.0 40.0 32.0 41.0 21 36.53386483328536 38.0 35.0 40.0 32.0 41.0 22 36.400918496943426 37.0 35.0 40.0 31.0 41.0 23 36.34553299578345 37.0 35.0 40.0 31.0 41.0 24 36.337914077184905 37.0 35.0 40.0 31.0 41.0 25 36.30099402231893 37.0 35.0 40.0 31.0 41.0 26 36.1696963744433 37.0 35.0 40.0 31.0 41.0 27 36.07045128951958 37.0 35.0 40.0 31.0 41.0 28 36.07271095456624 36.0 35.0 40.0 31.0 41.0 29 36.1283633346316 36.0 35.0 40.0 31.0 41.0 30 36.063956107225714 36.0 35.0 40.0 31.0 41.0 31 35.85950586761045 36.0 35.0 40.0 30.0 41.0 32 35.666592383113475 36.0 34.0 40.0 30.0 41.0 33 35.55319052376679 36.0 34.0 40.0 30.0 41.0 34 35.461517619765296 36.0 35.0 40.0 30.0 41.0 35 35.36400995715713 36.0 34.0 40.0 29.0 41.0 36 35.17924914906948 36.0 34.0 40.0 29.0 41.0 37 35.02674140179161 36.0 34.0 40.0 27.0 41.0 38 34.94947453982016 36.0 34.0 40.0 27.0 41.0 39 34.89887660237414 36.0 34.0 40.0 27.0 41.0 40 34.702835757709174 36.0 34.0 40.0 25.0 41.0 41 34.706449460653985 36.0 34.0 40.0 26.0 41.0 42 34.6383438605998 36.0 34.0 40.0 26.0 41.0 43 34.5533923763399 35.0 34.0 40.0 26.0 41.0 44 34.37240512759724 35.0 34.0 40.0 24.0 41.0 45 34.23629349906017 35.0 34.0 40.0 24.0 41.0 46 34.19523343437251 35.0 34.0 40.0 24.0 41.0 47 34.12430663979815 35.0 33.0 40.0 23.0 41.0 48 34.053704299527546 35.0 34.0 40.0 23.0 41.0 49 33.95294193351735 35.0 34.0 40.0 24.0 41.0 50 33.829489441687976 35.0 33.0 39.0 24.0 41.0 51 33.45202242053748 35.0 33.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 2.0 12 2.0 13 1.0 14 14.0 15 41.0 16 71.0 17 249.0 18 610.0 19 1106.0 20 2063.0 21 3451.0 22 5474.0 23 8084.0 24 12120.0 25 17543.0 26 21917.0 27 25081.0 28 27323.0 29 31283.0 30 37113.0 31 45394.0 32 56645.0 33 75848.0 34 147148.0 35 254563.0 36 94826.0 37 112840.0 38 172679.0 39 322772.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.798621577227237 25.187407921697456 29.71730479399861 13.296665707076693 2 29.08302711123906 27.097420960831798 31.490423856535653 12.329128071393495 3 28.21844783499568 25.62674207914924 32.60488036170897 13.549929724146107 4 24.864714747768954 28.448749428479502 31.896702961746225 14.789832862005317 5 22.344267014376914 32.73906490779469 31.009025790391682 13.907642287436708 6 20.780180515807835 40.874468697610624 27.690853978629367 10.654496807952178 7 89.17345435456285 3.610519363961187 5.817553722926863 1.3984725585491 8 89.90737134438555 2.524715086447767 5.273567811965522 2.2943457572011585 9 86.28560784380133 4.972888760943559 6.800264169474879 1.9412392257802316 10 52.064958596515 28.80510727651432 10.633566457250266 8.49636766972042 11 43.56249470814353 21.783414898481027 22.643252671329144 12.010837722046297 12 40.980813845189914 22.337696645386348 23.513521751646827 13.167967757776914 13 21.03222528914704 42.17905948893367 23.92799688415491 12.860718337764382 14 14.271112390564408 44.89634734899158 26.855672023436572 13.976868237007434 15 12.195214468358932 27.03747481076321 47.57062299967825 13.196687721199602 16 14.755964980610639 22.277547288029396 46.1674089377339 16.799078793626066 17 15.573535637478198 21.77007095321152 27.521446835893183 35.134946573417096 18 19.991939444228066 26.274702385992242 33.90242663370193 19.830931536077763 19 26.14417557109715 28.26816588488307 25.527576922425617 20.06008162159416 20 28.992464396389682 27.10426227287352 25.04421451916075 18.859058811576045 21 20.675528762298274 30.355172472186005 28.949452187018444 20.019846578497283 22 21.129764787563715 26.829051868660358 25.780231317629926 26.260952026146008 23 17.391360303456217 32.672751596023915 24.854215704536603 25.08167239598327 24 18.55831202479129 27.952110815707925 37.02477435524021 16.46480280426058 25 15.192454236025267 29.069005808341657 34.29143311940122 21.44710683623186 26 15.37466343792864 37.43118893197636 26.391952991380624 20.802194638714376 27 16.488645792762433 36.32763788461213 28.924660897837533 18.259055424787903 28 13.222698254110716 31.8370277547288 38.041149475894535 16.89912451526595 29 13.56964083111781 27.512167036391038 36.581985673886166 22.33620645860498 30 17.748530980644507 32.530167815352314 31.27143413543766 18.449867068565524 31 27.08224814996698 29.739047973853992 23.836892283203223 19.3418115929758 32 27.366806089445074 30.36296208490678 25.002015138942983 17.268216686705163 33 24.860989280815538 30.408751460552384 24.31185545188221 20.41840380674987 34 18.17817892401741 31.078793626064723 27.451814471745724 23.29121297817215 35 17.647062807986046 29.487612822379894 29.485716221021796 23.379608148612263 36 28.447394713223716 26.760232333666366 26.66594415186358 18.126428801246337 37 19.583966944947758 32.50009313667383 29.491202817807732 18.424737100570674 38 19.114151694240768 33.67910182378541 24.54710175604965 22.65964472592417 39 18.993040150373393 32.29227981643608 28.298579242375492 20.41610079081503 40 22.287233502108275 28.598242256955615 25.94442280663133 23.170101434304776 41 16.50889878583645 26.992091849694344 27.654479874011486 28.844529490457727 42 21.621865104228405 28.767378456640646 24.518991414492067 25.091765024638885 43 21.029515858635463 28.801246338035323 26.253433356476386 23.915804446852825 44 19.093424550827223 33.127055357052136 26.658290010668384 21.121230081452254 45 17.304861734374207 37.47081435320814 22.078065466614735 23.14625844580292 46 22.3838246998459 32.911113745279664 25.743721741486464 18.961339813387973 47 20.85353834690871 29.21687297851083 27.49326875857281 22.436319916007655 48 22.85905881157604 25.65844241613466 29.764584356425583 21.717914415863714 49 20.95046822346028 24.50063502277615 30.35029549726517 24.1986012564984 50 19.18561292398354 32.14109359389023 27.382791729463364 21.290501752662863 51 17.435185341980933 34.178314395542984 23.874553367314107 24.511946895161973 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1078.0 1 1375.0 2 1672.0 3 6698.0 4 11724.0 5 8644.5 6 5565.0 7 5793.5 8 6022.0 9 7189.5 10 8357.0 11 9217.5 12 10078.0 13 10392.0 14 10706.0 15 10316.5 16 9927.0 17 9334.5 18 8742.0 19 8277.5 20 7813.0 21 7803.0 22 7793.0 23 7859.0 24 7925.0 25 9142.5 26 11550.0 27 12740.0 28 14491.0 29 16242.0 30 18120.0 31 19998.0 32 25223.5 33 30449.0 34 34469.0 35 38489.0 36 41861.5 37 45234.0 38 53977.5 39 62721.0 40 85155.5 41 107590.0 42 130552.5 43 153515.0 44 161035.5 45 168556.0 46 161879.0 47 155202.0 48 149052.0 49 142902.0 50 130986.5 51 119071.0 52 103372.0 53 87673.0 54 74773.5 55 61874.0 56 53470.0 57 45066.0 58 40305.5 59 35545.0 60 30926.5 61 26308.0 62 21963.5 63 17619.0 64 13458.5 65 9298.0 66 7170.5 67 5043.0 68 3708.5 69 2374.0 70 2000.5 71 1627.0 72 1486.0 73 1345.0 74 1020.0 75 652.5 76 610.0 77 495.0 78 380.0 79 290.5 80 201.0 81 163.0 82 125.0 83 76.0 84 27.0 85 24.5 86 22.0 87 16.5 88 11.0 89 6.5 90 2.0 91 3.5 92 5.0 93 4.5 94 4.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1476325.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.668459255503368 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17194946382922 16.721972426247483 2 9.491761725953369 4.113437044435337 3 3.572227726326524 2.3221401281785714 4 1.695124195652923 1.469229182660931 5 0.959059118817748 1.0390666719860668 6 0.6220972876753672 0.808793383857171 7 0.4328865700020182 0.6565989503040336 8 0.3304781445940094 0.5728761768775714 9 0.2518404392251261 0.4911294866613945 >10 2.8693785604176165 14.795796766433526 >50 1.5783661029594018 25.534739376038484 >100 1.0185045493334908 29.71839893907036 >500 0.005060892170597412 0.7606412149086653 >1k 9.489172819870148E-4 0.40615965395104986 >5k 3.1630576066233825E-4 0.5890205983893557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8594 0.5821211454117488 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 3886 0.26322117419944796 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08419555314717288 0.0 2 0.0 0.0 0.0 0.3093492286590012 0.0 3 0.0 0.0 0.0 0.46995072223257073 0.0 4 0.0 0.0 0.0 0.8363334631602121 0.0 5 0.0 0.0 0.0 1.4388430731715578 0.0 6 0.0 0.0 0.0 2.0991312888422264 0.0 7 0.0 0.0 0.0 2.4373359524494944 0.0 8 0.0 0.0 0.0 3.260731884916939 0.0 9 0.0 0.0 0.0 3.5761773322269828 0.0 10 0.0 0.0 0.0 4.163175452559565 0.0 11 0.0 0.0 0.0 4.985284405534012 0.0 12 0.0 0.0 0.0 5.57695629349906 0.0 13 0.0 0.0 0.0 5.807325622745669 0.0 14 0.0 0.0 0.0 5.892943626911419 0.0 15 0.0 0.0 0.0 6.02692496570877 0.0 16 0.0 0.0 0.0 6.354156435744162 0.0 17 0.0 0.0 0.0 6.741401791610926 0.0 18 0.0 0.0 0.0 7.255448495419369 0.0 19 0.0 0.0 0.0 7.51833099080487 0.0 20 0.0 0.0 0.0 7.782094051106633 0.0 21 0.0 0.0 0.0 8.1107479721606 0.0 22 0.0 0.0 0.0 8.477740334953348 0.0 23 0.0 0.0 0.0 8.87880378642914 0.0 24 0.0 0.0 0.0 9.148595329619155 0.0 25 0.0 0.0 0.0 9.367720522242731 0.0 26 0.0 0.0 0.0 9.58650703605236 0.0 27 0.0 0.0 0.0 9.793913941713376 0.0 28 6.773576278935871E-5 0.0 0.0 9.99840820957445 0.0 29 6.773576278935871E-5 0.0 0.0 10.244966386127716 0.0 30 6.773576278935871E-5 0.0 0.0 10.567930503107378 0.0 31 6.773576278935871E-5 0.0 0.0 10.853436743264526 0.0 32 6.773576278935871E-5 0.0 0.0 11.098233789985267 0.0 33 6.773576278935871E-5 0.0 0.0 11.354952330956937 0.0 34 6.773576278935871E-5 0.0 0.0 11.618783127021489 0.0 35 6.773576278935871E-5 0.0 0.0 11.948724027568456 0.0 36 6.773576278935871E-5 0.0 0.0 12.22285065957699 0.0 37 6.773576278935871E-5 0.0 0.0 12.500160872436625 0.0 38 6.773576278935871E-5 0.0 0.0 12.767242985115066 0.0 39 6.773576278935871E-5 0.0 0.0 13.076321270722909 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGAG 25 3.891347E-5 45.0 1 TCACGAC 200 0.0 45.0 25 AAGTACG 25 3.891347E-5 45.0 1 AATCCGT 50 2.1827873E-11 45.0 33 AATCCGG 50 2.1827873E-11 45.0 2 GTCGACG 45 3.8562575E-10 45.0 1 CGAGTAC 20 7.0339395E-4 45.0 18 ACGTTGC 25 3.891347E-5 45.0 11 CGCGGAT 70 0.0 45.0 23 GACCGTA 20 7.0339395E-4 45.0 9 TAGCGCC 20 7.0339395E-4 45.0 19 GACCGAC 35 1.2122473E-7 45.0 9 CAACCGG 25 3.891347E-5 45.0 2 CCAGCGA 20 7.0339395E-4 45.0 21 AAGCGGT 20 7.0339395E-4 45.0 14 CGCGAGC 70 0.0 45.0 17 AGGCGAT 90 0.0 45.0 6 CCGCTCG 45 3.8562575E-10 45.0 19 AACGGTC 25 3.891347E-5 45.0 17 TGTTGCG 50 2.1827873E-11 45.0 1 >>END_MODULE