FastQCFastQC Report
Thu 26 May 2016
SRR1547876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547876_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2209547
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367671.6640062420034514No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.17333869793220058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT186100.043.4887161
GCGCGAC4400.041.4204569
CACGACG2800.039.37526
CGGTCTA2750.039.27272831
CGGTTTT19300.039.1709821
TATAGCG1400.038.5714261
TACGGGA8050.038.2919244
GGGCGAT28700.037.7090577
CGCGACC4850.037.5773210
TAGGGAC19850.037.2921945
CGGGTAT2300.037.1739126
CGAGGGA14050.037.1530274
GACCGAT9450.036.9047629
TCACGAC3050.036.88524225
TCGATAG552.752131E-936.818181
TATCGCG552.752131E-936.818181
ACGGGAT8250.036.5454525
AGGGCGA15900.036.509436
AGGGAAC22700.036.376656
TACGCGG1300.036.3461572
Produced by FastQC (version 0.11.5)