##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547876_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2209547
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46291796463257	31.0	31.0	33.0	30.0	34.0
2	31.831412502200678	31.0	31.0	34.0	30.0	34.0
3	31.619973686914104	31.0	31.0	34.0	30.0	34.0
4	35.57453179316846	37.0	35.0	37.0	33.0	37.0
5	35.61896216735828	37.0	35.0	37.0	33.0	37.0
6	35.70389586643778	37.0	35.0	37.0	33.0	37.0
7	35.985959112886036	37.0	35.0	37.0	35.0	37.0
8	36.01605170652627	37.0	35.0	37.0	35.0	37.0
9	37.827226123725815	39.0	38.0	39.0	35.0	39.0
10	37.35944245585181	39.0	37.0	39.0	34.0	39.0
11	37.13947112236128	39.0	37.0	39.0	33.0	39.0
12	36.73449535130957	39.0	35.0	39.0	33.0	39.0
13	36.6170495581221	39.0	35.0	39.0	33.0	39.0
14	37.65821138903133	40.0	36.0	41.0	33.0	41.0
15	37.805323444126785	40.0	36.0	41.0	33.0	41.0
16	37.864684933155985	40.0	36.0	41.0	33.0	41.0
17	37.783577357711785	40.0	36.0	41.0	33.0	41.0
18	37.6044569316697	39.0	36.0	41.0	33.0	41.0
19	37.43556937236456	39.0	36.0	41.0	33.0	41.0
20	37.199430471494836	39.0	35.0	41.0	32.0	41.0
21	37.11992277150022	39.0	35.0	41.0	32.0	41.0
22	37.06232680273377	39.0	35.0	41.0	32.0	41.0
23	36.96868226835637	39.0	35.0	41.0	32.0	41.0
24	36.970246389870866	39.0	35.0	41.0	32.0	41.0
25	36.917417009006826	39.0	35.0	41.0	32.0	41.0
26	36.830160209309874	39.0	35.0	41.0	32.0	41.0
27	36.77229676490249	38.0	35.0	41.0	31.0	41.0
28	36.67999775519598	38.0	35.0	41.0	31.0	41.0
29	36.63927176022958	38.0	35.0	41.0	31.0	41.0
30	36.488173820244604	38.0	35.0	41.0	31.0	41.0
31	36.27334516984703	38.0	35.0	41.0	30.0	41.0
32	36.02078706630816	38.0	35.0	41.0	30.0	41.0
33	35.76306138769621	38.0	35.0	41.0	28.0	41.0
34	35.43191839775302	38.0	35.0	41.0	25.0	41.0
35	35.20381055483318	38.0	34.0	41.0	24.0	41.0
36	35.05812096325627	38.0	34.0	40.0	23.0	41.0
37	34.94639987291513	38.0	34.0	40.0	23.0	41.0
38	34.79123910919297	38.0	34.0	40.0	23.0	41.0
39	34.67975743444244	38.0	34.0	40.0	22.0	41.0
40	34.54719451543687	38.0	34.0	40.0	22.0	41.0
41	34.44213451897606	38.0	34.0	40.0	21.0	41.0
42	34.384356612464	38.0	34.0	40.0	21.0	41.0
43	34.32898281865016	38.0	33.0	40.0	21.0	41.0
44	34.09998520058637	37.0	33.0	40.0	20.0	41.0
45	33.98895112889656	37.0	33.0	40.0	20.0	41.0
46	33.95621998536352	37.0	33.0	40.0	20.0	41.0
47	33.89114737093169	37.0	33.0	40.0	20.0	41.0
48	33.78771847804097	37.0	33.0	40.0	20.0	41.0
49	33.64488105480444	36.0	33.0	40.0	20.0	41.0
50	33.59505455190589	36.0	33.0	40.0	20.0	41.0
51	33.29867434365506	36.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	4.0
12	9.0
13	12.0
14	35.0
15	65.0
16	186.0
17	499.0
18	1005.0
19	1986.0
20	3477.0
21	5618.0
22	8778.0
23	13285.0
24	20830.0
25	33183.0
26	46925.0
27	52027.0
28	51449.0
29	50659.0
30	53508.0
31	60995.0
32	73312.0
33	94442.0
34	155130.0
35	201673.0
36	153376.0
37	205473.0
38	332774.0
39	588743.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.870852713248464	22.48017353783377	27.534920053748575	19.114053695169194
2	35.74945452619926	23.98803917726122	27.952562222030124	12.30994407450939
3	27.98116537009622	24.310412948898573	34.79052493565423	12.917896745350971
4	24.17319025121439	25.94880308045043	35.7488661703055	14.129140498029685
5	22.376079802783106	30.002213123323468	33.955014308362756	13.666692765530671
6	20.109642383710327	38.46842814386841	31.085195291161494	10.336734181259779
7	84.11158486332266	2.7185662943580744	11.691129448706002	1.478719393613261
8	85.04752331586519	2.02303911163691	11.338794784632325	1.590642787865567
9	81.08286449665927	3.748279624737559	12.598057429871371	2.5707984487317987
10	38.01982940394569	29.456897726094986	19.683808491061743	12.839464378897574
11	30.366722228583505	24.890169794985127	29.023415206827462	15.719692769603904
12	28.282946685451815	21.405428352508455	32.56635862464116	17.745266337398572
13	23.324871568697112	23.903994800744226	33.80412365068496	18.9670099798737
14	18.366434386777016	26.629892914701518	35.774165473737376	19.229507224784083
15	18.862282630783593	24.81282362402791	38.7316042609639	17.593289484224595
16	23.778584479081005	23.453359444266177	35.653145192204555	17.11491088444826
17	22.77534716392093	23.73110868426877	32.71915012443728	20.77439402737303
18	23.500473173913026	24.544759627199603	34.02787992289822	17.926887275989152
19	24.530005471709813	26.248095197793937	31.03871517555408	18.183184154942168
20	25.218879707016868	26.725749667239484	30.955349671222198	17.100020954521447
21	23.994103768781564	25.450420380286094	33.39729817921954	17.1581776717128
22	22.926147305307374	21.962013028009814	34.555227836294044	20.556611830388764
23	21.30576991573386	25.147733902017023	33.93813302002628	19.608363162222844
24	21.229193133253105	25.220373225824115	34.8305783945759	18.719855246346874
25	22.131278492831335	25.639508912912916	32.48118279448231	19.748029799773438
26	20.098689912457168	27.106959028253304	31.33529180415714	21.459059255132388
27	19.362611431211917	26.00202665976329	34.10472825425302	20.530633654771773
28	18.28741366442986	26.696241356259904	34.68634068431221	20.330004294998023
29	20.212106825516724	26.03366210358956	34.02638640409098	19.727844666802742
30	22.011978020834135	24.672885437603274	34.28567032065849	19.029466220904105
31	24.23768310880013	24.117794280909163	32.04647830528158	19.598044305009125
32	25.131893551031048	24.944886893105238	30.94403513480365	18.979184421060065
33	23.903677993724507	25.08926037780595	30.423159136239235	20.583902492230308
34	20.83187187237927	26.038504725176697	32.97503968007922	20.154583722364812
35	20.965066595098452	26.895196164643703	31.628066748523565	20.51167049173428
36	23.476350582268672	28.065390779195916	29.11221168864025	19.34604694989516
37	22.050764251676927	28.57757721379088	29.502246388060538	19.869412146471653
38	22.105209800922996	28.421527127506224	28.869809060409214	20.603454011161563
39	21.381305760864105	27.25400274354879	29.018708359677348	22.34598313590976
40	23.09206366734901	25.538266441039724	30.077432161433997	21.29223773017727
41	19.914670292145857	26.03615130160164	30.162562733447174	23.88661567280533
42	21.00973638487889	26.585992513397542	29.64155096044574	22.76272014127783
43	21.419639410250156	26.07760776303921	29.7007486149876	22.802004211723037
44	21.832710505818614	26.692303897586246	29.547866598900136	21.927118997695004
45	20.93447208862269	27.16072570531426	28.717786949089565	23.18701525697349
46	22.007814271432107	26.94995852090949	29.39643284347425	21.64579436418415
47	20.934924670079432	26.653517666743454	30.801290943347208	21.6102667198299
48	21.243856772451547	26.24881932812472	30.992189801801	21.515134097622727
49	21.944362351196876	25.594477057967087	30.80857750480076	21.652583086035282
50	20.16779910090168	26.487239239536432	31.221829632951913	22.12313202660998
51	19.770251549299473	26.96394328792282	30.545491903996613	22.7203132587811
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	786.0
1	1841.0
2	2896.0
3	31449.5
4	60003.0
5	42705.0
6	25407.0
7	22495.0
8	19583.0
9	19306.5
10	19030.0
11	18726.0
12	18422.0
13	17552.5
14	16683.0
15	15879.5
16	15076.0
17	14349.5
18	13623.0
19	13112.5
20	12602.0
21	12578.5
22	12555.0
23	13420.0
24	14285.0
25	15433.5
26	19811.5
27	23041.0
28	27026.5
29	31012.0
30	35979.0
31	40946.0
32	46484.5
33	52023.0
34	57316.5
35	62610.0
36	67375.5
37	72141.0
38	79749.5
39	87358.0
40	94886.5
41	102415.0
42	115409.0
43	128403.0
44	133211.0
45	138019.0
46	146408.0
47	154797.0
48	160495.0
49	166193.0
50	163983.5
51	161774.0
52	153365.0
53	144956.0
54	133544.0
55	122132.0
56	117421.0
57	112710.0
58	105476.5
59	98243.0
60	89936.0
61	81629.0
62	71214.0
63	60799.0
64	51926.5
65	43054.0
66	35583.0
67	28112.0
68	23088.0
69	18064.0
70	15207.0
71	12350.0
72	10357.0
73	8364.0
74	6859.5
75	4090.0
76	2825.0
77	2132.5
78	1440.0
79	1059.0
80	678.0
81	472.5
82	267.0
83	199.0
84	131.0
85	116.0
86	101.0
87	69.0
88	37.0
89	25.5
90	14.0
91	15.0
92	16.0
93	10.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2209547.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.883196126899882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22740917309862	17.972487881920607
2	6.878839019951173	3.4233500091763367
3	3.099448839679287	2.3137258008909596
4	1.9260175591114301	1.917018906688908
5	1.4034476650926795	1.746113175217042
6	1.1913787494163648	1.778718664988887
7	0.9718004650363553	1.69270510973985
8	0.8756705682361171	1.7431585993578536
9	0.7929229750130171	1.775741211069646
>10	10.14737163699005	54.60946628132211
>50	0.4471437905713881	6.673063029164047
>100	0.03410168382975102	1.6294834701761727
>500	0.003335905480360457	0.5976575303659878
>1k	9.266404112112381E-4	0.43177989635931663
>5k	0.0	0.0
>10k+	1.8532808224224758E-4	1.6955304335622126
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36767	1.6640062420034514	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3830	0.17333869793220058	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5258145674203805E-5	0.0	0.0	0.031001829786829607	0.0
2	4.5258145674203805E-5	0.0	0.0	0.09549468737257004	0.0
3	4.5258145674203805E-5	0.0	0.0	0.17361024680624582	0.0
4	4.5258145674203805E-5	0.0	0.0	0.2721824880846617	0.0
5	4.5258145674203805E-5	0.0	0.0	0.5676276630458642	0.0
6	4.5258145674203805E-5	0.0	0.0	0.9556257459108134	0.0
7	4.5258145674203805E-5	0.0	0.0	1.2321982741258728	0.0
8	4.5258145674203805E-5	0.0	0.0	1.7366003076648744	0.0
9	4.5258145674203805E-5	0.0	0.0	2.129214721388592	0.0
10	4.5258145674203805E-5	0.0	0.0	2.667877171202966	0.0
11	4.5258145674203805E-5	0.0	0.0	3.0481813693033004	0.0
12	4.5258145674203805E-5	0.0	0.0	3.336747306122024	0.0
13	4.5258145674203805E-5	0.0	0.0	3.4845151517482997	0.0
14	4.5258145674203805E-5	0.0	0.0	3.592772636200995	0.0
15	4.5258145674203805E-5	0.0	0.0	3.672245940004897	0.0
16	4.5258145674203805E-5	0.0	0.0	3.7784668079022534	0.0
17	4.5258145674203805E-5	0.0	0.0	3.8933772397690567	0.0
18	4.5258145674203805E-5	0.0	0.0	4.041778699434771	0.0
19	4.5258145674203805E-5	0.0	0.0	4.14134661991802	0.0
20	4.5258145674203805E-5	0.0	0.0	4.252500625693864	0.0
21	4.5258145674203805E-5	0.0	0.0	4.374199779411798	0.0
22	4.5258145674203805E-5	0.0	0.0	4.513006512194581	0.0
23	4.5258145674203805E-5	0.0	0.0	4.658104127226078	0.0
24	4.5258145674203805E-5	0.0	0.0	4.780165346109406	0.0
25	4.5258145674203805E-5	0.0	0.0	4.886386214006762	0.0
26	4.5258145674203805E-5	0.0	0.0	4.990932530514173	0.0
27	4.5258145674203805E-5	0.0	0.0	5.119737213102957	0.0
28	4.5258145674203805E-5	0.0	0.0	5.241979464568982	0.0
29	4.5258145674203805E-5	0.0	0.0	5.384678397879746	0.0
30	4.5258145674203805E-5	0.0	0.0	5.55901277501678	0.0
31	1.8103258269681522E-4	0.0	0.0	5.697547958925517	0.0
32	1.8103258269681522E-4	0.0	0.0	5.860432025206977	0.0
33	1.8103258269681522E-4	0.0	0.0	6.013992913479551	0.0
34	1.8103258269681522E-4	0.0	0.0	6.180316598832249	0.0
35	1.8103258269681522E-4	0.0	0.0	6.35623501106788	0.0
36	1.8103258269681522E-4	0.0	0.0	6.529528450854406	0.0
37	1.8103258269681522E-4	0.0	0.0	6.713186006000325	0.0
38	1.8103258269681522E-4	0.0	0.0	6.9433689348993255	0.0
39	1.8103258269681522E-4	0.0	0.0	7.326207589157415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18610	0.0	43.488716	1
GCGCGAC	440	0.0	41.420456	9
CACGACG	280	0.0	39.375	26
CGGTCTA	275	0.0	39.272728	31
CGGTTTT	1930	0.0	39.170982	1
TATAGCG	140	0.0	38.571426	1
TACGGGA	805	0.0	38.291924	4
GGGCGAT	2870	0.0	37.709057	7
CGCGACC	485	0.0	37.57732	10
TAGGGAC	1985	0.0	37.292194	5
CGGGTAT	230	0.0	37.173912	6
CGAGGGA	1405	0.0	37.153027	4
GACCGAT	945	0.0	36.904762	9
TCACGAC	305	0.0	36.885242	25
TCGATAG	55	2.752131E-9	36.81818	1
TATCGCG	55	2.752131E-9	36.81818	1
ACGGGAT	825	0.0	36.545452	5
AGGGCGA	1590	0.0	36.50943	6
AGGGAAC	2270	0.0	36.37665	6
TACGCGG	130	0.0	36.346157	2
>>END_MODULE