FastQCFastQC Report
Thu 26 May 2016
SRR1547875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547875_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3853497
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196200.5091479245993963No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47170.12240829563381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG302.1668366E-645.0000041
TACGTCA207.035356E-445.033
CGGTCTA7400.041.35135331
CGTTTTT104050.040.9130251
CGTAAGG5450.040.045872
TACGGGA15300.039.7058834
TCACGAC8400.039.37525
ACCGTTC9100.038.8186845
TACGTAG1800.038.751
AACGGGA13000.037.9038474
TAAGGGA41200.037.8458754
CACGACG8550.037.6315826
CGAATAT4450.037.4157314
TAGGGAT49950.037.3423425
TATGGGA39900.037.2180444
GGGCGAT60250.037.195027
GGTTACG1150.037.1739121
AGGGCGA28700.037.081886
TAGGGAG45350.037.061745
ATAGGGA48450.036.9195064
Produced by FastQC (version 0.11.5)