FastQCFastQC Report
Thu 26 May 2016
SRR1547874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547874_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1890401
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87560.46318215024219733No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38290.20254961777950814No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC253.8917642E-545.012
ACCGGAT207.0344436E-445.012
GTCGAAT207.0344436E-445.040
TCCGATT351.2124838E-745.027
CGCGATT207.0344436E-445.038
CCCGTTA453.8562575E-1045.030
CCCGGTA207.0344436E-445.023
TCGCGAC253.8917642E-545.010
CCGAACG302.1662472E-644.9999961
CGCATCG1050.042.85714721
CGTTTTT48650.042.687561
TACGGGT1850.042.5675664
TACGGGA7700.040.9090924
TACGATG1100.040.9090921
TTACGGG6150.040.6097563
GCGATGA1500.040.59
AGGGCGA15100.040.3807956
CGACGGT2800.040.1785728
AACGGGA4500.040.04
ACGGGAT7150.039.9650345
Produced by FastQC (version 0.11.5)