##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547874_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1890401
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44366248219293	31.0	31.0	33.0	30.0	34.0
2	31.853814084948116	31.0	31.0	34.0	30.0	34.0
3	31.919823889217156	33.0	31.0	34.0	30.0	34.0
4	35.69193784810736	37.0	35.0	37.0	33.0	37.0
5	35.59964684741492	37.0	35.0	37.0	33.0	37.0
6	35.641454908244334	37.0	35.0	37.0	33.0	37.0
7	35.99539198297081	37.0	35.0	37.0	35.0	37.0
8	36.02630024000199	37.0	35.0	37.0	35.0	37.0
9	37.81817032470889	39.0	38.0	39.0	35.0	39.0
10	37.2397089294811	39.0	37.0	39.0	34.0	39.0
11	37.013477034766694	39.0	37.0	39.0	33.0	39.0
12	36.37062718439104	38.0	35.0	39.0	32.0	39.0
13	36.1394995030155	38.0	35.0	39.0	32.0	39.0
14	37.21058336300076	39.0	35.0	41.0	32.0	41.0
15	37.41575729170689	39.0	35.0	41.0	33.0	41.0
16	37.51550120847376	39.0	35.0	41.0	33.0	41.0
17	37.43703372988059	39.0	35.0	41.0	33.0	41.0
18	37.36586099986194	39.0	35.0	41.0	32.0	41.0
19	37.289125428943386	39.0	35.0	41.0	32.0	41.0
20	37.13943814037339	39.0	35.0	41.0	32.0	41.0
21	37.031156352541075	39.0	35.0	41.0	32.0	41.0
22	36.9166007635417	38.0	35.0	41.0	32.0	41.0
23	36.841932478876174	38.0	35.0	41.0	32.0	41.0
24	36.85755244522194	38.0	35.0	41.0	32.0	41.0
25	36.81785822161542	38.0	35.0	41.0	32.0	41.0
26	36.70924687407592	38.0	35.0	41.0	31.0	41.0
27	36.62509382929865	38.0	35.0	41.0	31.0	41.0
28	36.59859786362787	38.0	35.0	41.0	31.0	41.0
29	36.62019698466093	38.0	35.0	41.0	31.0	41.0
30	36.54488439225329	38.0	35.0	40.0	31.0	41.0
31	36.408319716293	38.0	35.0	40.0	31.0	41.0
32	36.254282027993	38.0	35.0	40.0	30.0	41.0
33	36.16561512610287	38.0	35.0	40.0	30.0	41.0
34	36.05682074861365	38.0	35.0	40.0	30.0	41.0
35	35.968452196121355	38.0	35.0	40.0	30.0	41.0
36	35.843802981483826	38.0	35.0	40.0	30.0	41.0
37	35.7564976954625	38.0	35.0	40.0	29.0	41.0
38	35.64532075469702	38.0	35.0	40.0	29.0	41.0
39	35.56381370936643	38.0	35.0	40.0	29.0	41.0
40	35.38563246633915	38.0	34.0	40.0	27.0	41.0
41	35.3349569747371	38.0	34.0	40.0	27.0	41.0
42	35.214266708492005	38.0	34.0	40.0	27.0	41.0
43	35.116220315160646	37.0	34.0	40.0	27.0	41.0
44	34.95803853256531	37.0	34.0	40.0	26.0	41.0
45	34.800244498389496	37.0	34.0	40.0	25.0	41.0
46	34.71446269865494	37.0	34.0	40.0	24.0	41.0
47	34.63304082043968	37.0	34.0	40.0	24.0	41.0
48	34.55840850697815	37.0	34.0	40.0	24.0	41.0
49	34.43986328826529	36.0	34.0	40.0	24.0	41.0
50	34.34805895680334	36.0	34.0	40.0	24.0	41.0
51	33.99641187240167	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	1.0
13	2.0
14	11.0
15	50.0
16	96.0
17	268.0
18	630.0
19	1197.0
20	2169.0
21	3584.0
22	5688.0
23	8692.0
24	12923.0
25	18995.0
26	24812.0
27	29566.0
28	33323.0
29	38027.0
30	45618.0
31	54773.0
32	67810.0
33	88310.0
34	154607.0
35	248242.0
36	129179.0
37	162739.0
38	260557.0
39	498467.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.870539636828376	24.73284768681354	30.43534149632803	12.961271180030057
2	29.458564611423714	26.768447541024365	30.76617077540691	13.00681707214501
3	28.762521814154773	25.5468019748191	32.11350396027086	13.577172250755263
4	24.911010944238814	28.156618622186507	31.810975554921946	15.12139487865273
5	22.76104382086129	32.420793260265945	30.50405707572097	14.314105843151797
6	20.017498932766117	40.40470778422144	28.06441596253916	11.513377320473275
7	88.4008736770664	3.645311232907727	6.2004833895030735	1.753331700522799
8	89.49043086625537	2.428162067201615	5.4247749551550175	2.6566321113880074
9	85.83348189087924	3.979473138238924	7.556068791753708	2.630976179128132
10	42.956124123929264	28.33747971991128	14.65979969329259	14.046596462866873
11	35.7356455059006	26.206344579800795	23.946347891267514	14.111662023031094
12	33.536852762985205	23.199416420114037	26.539501407373358	16.724229409527396
13	20.468091161610687	34.89069250386558	27.476657069055722	17.164559265468014
14	14.934291719058548	37.83059784670025	29.472477003556392	17.76263343068481
15	13.929742948718287	28.037702053691255	42.37185655318633	15.660698444404122
16	16.437411956510815	23.85530900586701	40.871857346668776	18.835421690953403
17	17.008349022244488	24.87070203623464	28.427249033406138	29.693699908114734
18	20.167149721143822	26.312195137433804	33.15026811771683	20.370387023705554
19	24.11752850321175	28.450101327707717	26.83139714801251	20.600973021068018
20	25.916088702873097	26.873504616216348	27.06970637446764	20.14070030644292
21	21.3129912648163	29.934548278381147	28.99908537923964	19.75337507756291
22	21.44957604233176	25.098325699150603	28.47443478923255	24.977663469285087
23	18.999831252734207	30.109326010724708	27.355518749725587	23.535323986815495
24	18.974598511109548	26.469727851392378	35.881699173878985	18.673974463619096
25	17.48983416745971	28.165399827867212	32.21649798111618	22.128268023556906
26	17.03458684162778	34.250457971615546	27.54452626717823	21.170428919578438
27	17.48581385642517	33.143602865212195	29.474804552050067	19.895778726312567
28	15.819394932609537	29.372551114816382	36.10926993796554	18.698784014608542
29	15.920696190913992	25.643130743159787	35.28732792672031	23.148845139205914
30	18.148001402876957	29.64492718740627	31.732050501454452	20.47502090826232
31	24.393237202053957	27.792568878243294	26.563676172409984	21.250517747292772
32	24.1922216503271	28.629798651185652	26.622182277728374	20.555797420758875
33	23.004854525574203	28.958564875917858	26.72745094823797	21.30912965026997
34	17.79035241729136	28.438728079386333	29.28595573108563	24.484963772236686
35	18.721424713592512	27.43878150720403	30.225650536579273	23.614143242624184
36	23.998559035887094	26.467611898216305	29.177671827300134	20.356157238596467
37	19.072143952526474	29.50865980286722	30.42994581572904	20.98925042887726
38	18.703650706913507	31.29246122912546	27.483533916877956	22.52035414708308
39	19.16318283792698	29.810817916410326	28.308067970763872	22.717931274898817
40	21.76331899951386	27.133872654532027	27.97073213566857	23.132076210285543
41	18.025329017494172	24.51527480148392	30.03246401160389	27.426932169418023
42	21.02881875326981	25.74739433591074	27.2738429571292	25.949943953690248
43	20.008453232938407	27.27849805411656	28.468563019168947	24.24448569377608
44	19.28305158535147	29.986071738218506	27.877259904115583	22.853616772314446
45	18.541674491285182	32.68184898336385	25.029874613904667	23.7466019114463
46	21.84123897522272	30.339912008087172	27.00278935527436	20.816059661415753
47	20.618588331258817	27.31235330493372	28.414553314349707	23.65450504945776
48	20.463224469305718	25.105467041119844	31.28632496491485	23.144983524659583
49	19.95756455905387	24.212852193793804	31.352184007520094	24.477399239632224
50	18.642552558954424	28.969514933604035	29.616520516017502	22.771411991424042
51	17.627318225074998	29.55547526688782	27.241415974705895	25.57579053333129
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1024.0
1	1557.5
2	2091.0
3	7860.0
4	13629.0
5	10162.0
6	6695.0
7	6589.5
8	6484.0
9	7077.0
10	7670.0
11	8080.5
12	8491.0
13	8573.5
14	8656.0
15	8526.5
16	8397.0
17	8543.5
18	8690.0
19	8213.5
20	7737.0
21	8074.5
22	8412.0
23	9085.0
24	9758.0
25	11996.0
26	17480.0
27	20726.0
28	24781.5
29	28837.0
30	33553.0
31	38269.0
32	43141.0
33	48013.0
34	54744.5
35	61476.0
36	64274.5
37	67073.0
38	75920.0
39	84767.0
40	104235.0
41	123703.0
42	146089.0
43	168475.0
44	175932.5
45	183390.0
46	186550.5
47	189711.0
48	183872.0
49	178033.0
50	167267.0
51	156501.0
52	138452.0
53	120403.0
54	102699.0
55	84995.0
56	75016.0
57	65037.0
58	57946.5
59	50856.0
60	44249.0
61	37642.0
62	32702.5
63	27763.0
64	22717.0
65	17671.0
66	14185.0
67	10699.0
68	8381.5
69	6064.0
70	4952.0
71	3840.0
72	2960.0
73	2080.0
74	1681.5
75	890.5
76	498.0
77	406.0
78	314.0
79	262.0
80	210.0
81	130.0
82	50.0
83	39.0
84	28.0
85	20.0
86	12.0
87	7.5
88	3.0
89	5.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1890401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.1267473386222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41913642827892	16.144877871559483
2	9.590978801258393	4.052523717285354
3	3.46638622701771	2.1970039798884917
4	1.7477695026219355	1.476987387521721
5	1.0146824520688165	1.071846989689576
6	0.6668710387440163	0.8453289565793619
7	0.441997566749562	0.6536579641902658
8	0.3418443762111815	0.5777647812274036
9	0.2668782425780543	0.507444228100987
>10	3.181673021497654	16.836897638568225
>50	2.034184209342672	31.506364050235717
>100	0.8217484848849779	22.455866919164748
>500	0.003560655758480712	0.5259844678272957
>1k	0.00203466043341755	0.6769719389269688
>5k	2.5433255417719374E-4	0.4704791092344265
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8756	0.46318215024219733	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3829	0.20254961777950814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.289882940180417E-5	0.0	0.0	0.10712012953865344	0.0
2	5.289882940180417E-5	0.0	0.0	0.3819824471104279	0.0
3	2.6449414700902083E-4	0.0	0.0	0.5732646142273518	0.0
4	2.6449414700902083E-4	0.0	0.0	1.0103676415744596	0.0
5	2.6449414700902083E-4	0.0	0.0	1.6803842147777113	0.0
6	2.6449414700902083E-4	0.0	0.0	2.5495648806787554	0.0
7	2.6449414700902083E-4	0.0	0.0	2.9666192516825793	0.0
8	2.6449414700902083E-4	0.0	0.0	4.013381287885481	0.0
9	2.6449414700902083E-4	0.0	0.0	4.412873247527905	0.0
10	2.6449414700902083E-4	0.0	0.0	5.165835185233186	0.0
11	2.6449414700902083E-4	0.0	0.0	5.996188110353306	0.0
12	2.6449414700902083E-4	0.0	0.0	6.6312914561513665	0.0
13	2.6449414700902083E-4	0.0	0.0	6.889279047143965	0.0
14	2.6449414700902083E-4	0.0	0.0	6.9856607143140526	0.0
15	2.6449414700902083E-4	0.0	0.0	7.136369479279793	0.0
16	2.6449414700902083E-4	0.0	0.0	7.490580040954273	0.0
17	2.6449414700902083E-4	0.0	0.0	7.896684354271924	0.0
18	2.6449414700902083E-4	0.0	0.0	8.433607472700237	0.0
19	2.6449414700902083E-4	0.0	0.0	8.704661074555082	0.0
20	2.6449414700902083E-4	0.0	0.0	8.995445939776799	0.0
21	2.6449414700902083E-4	0.0	0.0	9.388748736379213	0.0
22	2.6449414700902083E-4	0.0	0.0	9.769408712754595	0.0
23	2.6449414700902083E-4	0.0	0.0	10.184611624729357	0.0
24	2.6449414700902083E-4	0.0	0.0	10.482749427237925	0.0
25	2.6449414700902083E-4	0.0	0.0	10.74507472224147	0.0
26	2.6449414700902083E-4	0.0	0.0	10.993857916918156	0.0
27	2.6449414700902083E-4	0.0	0.0	11.23058017849123	0.0
28	2.6449414700902083E-4	0.0	0.0	11.474337984374744	0.0
29	2.6449414700902083E-4	0.0	0.0	11.74338143071232	0.0
30	2.6449414700902083E-4	0.0	0.0	12.09817387951022	0.0
31	2.6449414700902083E-4	0.0	0.0	12.412816116792152	0.0
32	2.6449414700902083E-4	0.0	0.0	12.683658123329389	0.0
33	2.6449414700902083E-4	0.0	0.0	12.94730588906798	0.0
34	2.6449414700902083E-4	0.0	0.0	13.228992155632588	0.0
35	2.6449414700902083E-4	0.0	0.0	13.567967854439349	0.0
36	2.6449414700902083E-4	0.0	0.0	13.85711285594961	0.0
37	2.6449414700902083E-4	0.0	0.0	14.149167293076971	0.0
38	3.17392976410825E-4	0.0	0.0	14.44852176866178	0.0
39	3.17392976410825E-4	0.0	0.0	14.745284201605903	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	25	3.8917642E-5	45.0	12
ACCGGAT	20	7.0344436E-4	45.0	12
GTCGAAT	20	7.0344436E-4	45.0	40
TCCGATT	35	1.2124838E-7	45.0	27
CGCGATT	20	7.0344436E-4	45.0	38
CCCGTTA	45	3.8562575E-10	45.0	30
CCCGGTA	20	7.0344436E-4	45.0	23
TCGCGAC	25	3.8917642E-5	45.0	10
CCGAACG	30	2.1662472E-6	44.999996	1
CGCATCG	105	0.0	42.857147	21
CGTTTTT	4865	0.0	42.68756	1
TACGGGT	185	0.0	42.567566	4
TACGGGA	770	0.0	40.909092	4
TACGATG	110	0.0	40.909092	1
TTACGGG	615	0.0	40.609756	3
GCGATGA	150	0.0	40.5	9
AGGGCGA	1510	0.0	40.380795	6
CGACGGT	280	0.0	40.17857	28
AACGGGA	450	0.0	40.0	4
ACGGGAT	715	0.0	39.965034	5
>>END_MODULE