FastQCFastQC Report
Thu 26 May 2016
SRR1547873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547873_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2503790
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101240.40434700993294165No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47290.18887366752003962No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC207.0348824E-445.023
TAATTCG207.0348824E-445.030
TATAGCG2050.042.8048821
CGGTCTA3350.041.64179231
TCGATAG1850.041.3513531
CGTTTTT61350.040.78241
TAAACGG1600.040.781252
CGTTAGG4200.039.642862
AGTACGG2400.039.3750042
CTATACG403.460409E-739.3751
CACGACG3600.039.37526
TGCACGG2600.038.9423072
AGGGCGA18450.038.658536
CGAATCT356.251241E-638.57142618
ATAACGG1950.038.076922
CGACGGT3800.037.89473328
TATACGG2800.037.7678572
GCGCGAC3350.037.6119429
AGGGTAC4550.037.5824176
CGAATTT301.1403061E-437.50000438
Produced by FastQC (version 0.11.5)