FastQCFastQC Report
Thu 26 May 2016
SRR1547872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547872_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3207990
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143850.4484116222307426No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35140.10953899482230307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1500.042.0000041
CGGTCTA5050.041.43564631
CGTTTTT77600.041.2596661
TAGGGAC33800.040.6065065
ACGGGTA2500.039.65
TACGGGA13800.039.4565244
AGGGATT60950.038.872036
GGGCGAT40350.038.4758387
AGGGCGA21900.038.321926
CACGACG5500.038.04545626
TAAGGGA34350.037.8602644
TCGTTAG900.037.51
TAGGGAT46000.037.4184765
CGACGGT5600.037.3660728
CGGGTAT2000.037.1256
GTAGGGA46800.036.8269234
TCACGAC5750.036.78260825
CGAGGGA21850.036.7620124
AAGGGAT51750.036.5652165
ATAGGGA42250.036.4792944
Produced by FastQC (version 0.11.5)