FastQCFastQC Report
Thu 26 May 2016
SRR1547871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547871_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1871538
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78400.4189068028541232No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35360.18893551720563515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA253.8917482E-545.0000049
GACCGAC253.8917482E-545.0000049
GCGGATA253.8917482E-545.00000430
ACGTACG453.8562575E-1045.0000041
AACCGGT207.0344255E-445.037
ATCCGAC207.0344255E-445.032
CCGTATT207.0344255E-445.019
CGACTCC207.0344255E-445.028
TAATGCG750.045.01
GCGTAAG1400.045.01
AGTCGAC207.0344255E-445.038
CGACGGT3050.042.78688428
TATAGCG850.042.3529431
ATAACGG1250.041.42
CGGTCTA3100.041.37096831
ACGCTAG603.6379788E-1241.2500041
CGTTTTT37750.041.1258241
TACACGG2150.040.8139532
CGCATCG1600.040.7812521
TAAGGGA20150.039.5285344