##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547871_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1871538
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45698137040231	31.0	31.0	33.0	30.0	34.0
2	31.8715014068643	31.0	31.0	34.0	30.0	34.0
3	31.966298306526504	33.0	31.0	34.0	30.0	34.0
4	35.72515546037537	37.0	35.0	37.0	35.0	37.0
5	35.59753956371711	37.0	35.0	37.0	33.0	37.0
6	35.648585815516434	37.0	35.0	37.0	33.0	37.0
7	35.98605852512746	37.0	35.0	37.0	35.0	37.0
8	36.01354126926624	37.0	35.0	37.0	35.0	37.0
9	37.7961115403481	39.0	38.0	39.0	35.0	39.0
10	37.28960672986602	39.0	37.0	39.0	34.0	39.0
11	37.014557545719086	39.0	37.0	39.0	33.0	39.0
12	36.283359461576524	38.0	35.0	39.0	32.0	39.0
13	36.002814262921724	38.0	35.0	39.0	32.0	39.0
14	36.99698910735449	39.0	35.0	41.0	32.0	41.0
15	37.24435304011995	39.0	35.0	41.0	32.0	41.0
16	37.366004323716645	39.0	35.0	41.0	33.0	41.0
17	37.29520479947509	39.0	35.0	41.0	33.0	41.0
18	37.22745891347117	39.0	35.0	41.0	32.0	41.0
19	37.15490949155187	39.0	35.0	41.0	32.0	41.0
20	37.00964126830446	38.0	35.0	41.0	32.0	41.0
21	36.8777128757204	38.0	35.0	41.0	32.0	41.0
22	36.770106190737245	38.0	35.0	41.0	31.0	41.0
23	36.701303419967964	38.0	35.0	41.0	31.0	41.0
24	36.724246047902845	38.0	35.0	41.0	32.0	41.0
25	36.68494094162128	38.0	35.0	41.0	32.0	41.0
26	36.57821855607527	38.0	35.0	41.0	31.0	41.0
27	36.476279936608286	38.0	35.0	40.0	31.0	41.0
28	36.42612225880532	38.0	35.0	40.0	31.0	41.0
29	36.456711538852005	38.0	35.0	40.0	31.0	41.0
30	36.36410000758734	38.0	35.0	40.0	31.0	41.0
31	36.20149951537185	38.0	35.0	40.0	30.0	41.0
32	36.07854075097593	38.0	35.0	40.0	30.0	41.0
33	35.96667927661635	38.0	35.0	40.0	30.0	41.0
34	35.86566663353883	38.0	35.0	40.0	30.0	41.0
35	35.78767783502125	38.0	35.0	40.0	30.0	41.0
36	35.67196872305024	38.0	35.0	40.0	29.0	41.0
37	35.54555451185068	38.0	34.0	40.0	29.0	41.0
38	35.48825351128323	38.0	34.0	40.0	29.0	41.0
39	35.422114325223426	37.0	34.0	40.0	28.0	41.0
40	35.26514983933001	37.0	34.0	40.0	27.0	41.0
41	35.21756651481295	37.0	34.0	40.0	27.0	41.0
42	35.1510458243434	37.0	34.0	40.0	27.0	41.0
43	35.084953658434934	37.0	34.0	40.0	27.0	41.0
44	34.87332557500836	37.0	34.0	40.0	26.0	41.0
45	34.71324653840852	36.0	34.0	40.0	26.0	41.0
46	34.69748356699142	36.0	34.0	40.0	26.0	41.0
47	34.61203405968781	36.0	34.0	40.0	26.0	41.0
48	34.550058294301266	36.0	34.0	40.0	25.0	41.0
49	34.42201173580232	36.0	34.0	40.0	24.0	41.0
50	34.30151832343238	36.0	34.0	40.0	24.0	41.0
51	33.951003399343215	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	12.0
14	25.0
15	46.0
16	119.0
17	311.0
18	613.0
19	1225.0
20	2338.0
21	3842.0
22	5797.0
23	8810.0
24	13159.0
25	18627.0
26	24498.0
27	29196.0
28	32930.0
29	38303.0
30	45703.0
31	55022.0
32	68575.0
33	89933.0
34	162864.0
35	262039.0
36	128279.0
37	162620.0
38	255538.0
39	461050.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.604006971806076	24.956052188093427	30.685724788916925	12.754216051183572
2	28.578473960988237	26.653639947465667	31.420735245557395	13.3471508459887
3	28.762066279177873	25.438703355208393	32.205490885036795	13.59373948057694
4	25.730709181432598	28.640348205593476	30.917031874319413	14.711910738654518
5	23.218337004111056	32.85249885388381	29.496008096015146	14.433156045989984
6	21.028480319395065	40.68701784307879	26.990635509404566	11.293866328121577
7	88.61316200900008	3.6867004570572437	5.9210125575863275	1.7791249763563444
8	89.32167019852122	2.686613897233185	5.368365483361813	2.6233504208837863
9	85.3551998409864	4.741661670775587	7.08112792793948	2.8220105602985353
10	46.69549856855698	27.966570809676323	12.69832618947625	12.639604432290447
11	38.32986559717195	23.239068616293125	22.730770093901377	15.700295692633546
12	36.563136842532714	21.820663005506702	25.250355589894514	16.365844562066066
13	22.158673775258638	34.80971265344332	25.924613873723107	17.106999697574935
14	15.761368457386386	37.762097269732166	28.079632900854808	18.396901372026644
15	14.805951041336057	26.343680972547713	42.3500885368077	16.500279449308536
16	17.680057792040557	22.822673116976517	40.771547251511855	18.725721839471067
17	17.90062504742089	23.963232378931128	26.92053273831469	31.21560983533329
18	21.220782051980777	25.674765887735113	31.843382287722715	21.26106977256139
19	25.295505621579682	27.804992471432588	25.449069161299427	21.450432745688307
20	27.807076319048825	25.781950459996004	25.813795926131345	20.59717729482383
21	22.424230766353663	29.300286716059198	28.11837109372078	20.15711142386636
22	22.218731332198438	24.569097715354964	27.04486897941693	26.167301973029666
23	19.514217718261666	29.744627146229462	26.66817344878918	24.072981686719693
24	20.11329719193519	25.39526314720834	35.299630571220035	19.19180908963644
25	18.15651084829696	27.428991556676912	31.899165285449723	22.5153323095764
26	17.33809305501678	33.20685981262469	26.58973528723435	22.865311845124168
27	18.318356346491495	33.131627570479466	28.148453304180837	20.4015627788482
28	15.85941615932992	29.17039354798032	35.64458749969276	19.32560279299699
29	16.700649412408406	26.07149841467285	34.00652297735873	23.221329195560013
30	19.29514655860581	29.763114614824815	30.614393082053372	20.327345744516006
31	26.199361167125645	27.848218951472	25.415193279538006	20.53722660186435
32	27.047647442905248	28.168062844569548	25.3046959238872	19.47959378863801
33	25.74502895479547	28.55800950875697	25.8162537976787	19.880707738768862
34	19.973999993588162	26.865604652430246	29.315942289176068	23.844453064805524
35	20.21583318105216	26.187819857251093	30.609584202938972	22.98676275875777
36	27.330035510900657	25.54433839975464	27.825670651624492	19.299955437720207
37	20.675562024388498	30.048013986357745	30.1732585712927	19.10316541796106
38	21.18594439439648	29.790311497816234	26.94992033290267	22.073823774884612
39	21.366491089147	28.670270120083053	28.972214296477016	20.99102449429293
40	23.68063058297507	25.61155584337588	27.556854309129708	23.15095926451934
41	18.17457086097103	24.49840719237333	29.451819840152858	27.875202106502776
42	21.695632148532383	26.19562092781445	26.50109161555897	25.607655308094195
43	21.948792917910296	26.39438793120952	27.017832392395984	24.6389867584842
44	20.53482216230715	29.687935804669742	27.566311771388026	22.210930261635085
45	19.102631098059458	32.335277189135354	24.356010938597024	24.206080774208164
46	22.960955107510507	29.94056225414605	26.853315294693452	20.24516734364998
47	21.422380950854325	26.585460728021555	28.75063183328364	23.241526487840485
48	22.65478980389391	23.962217171118088	30.876209833837198	22.50678319115081
49	21.617674874888994	23.460918239437298	30.6885566843954	24.232850201278307
50	19.598533398734087	28.346151667772705	29.710163512576287	22.345151420916913
51	18.605179269670185	29.38887695574442	26.907228172764857	25.098715601820537
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	941.0
1	1570.5
2	2200.0
3	7340.0
4	12480.0
5	9368.5
6	6257.0
7	6184.5
8	6112.0
9	6851.0
10	7590.0
11	7908.0
12	8226.0
13	8265.5
14	8305.0
15	7925.5
16	7546.0
17	6969.5
18	6393.0
19	6698.0
20	7003.0
21	6806.0
22	6609.0
23	8113.5
24	9618.0
25	10413.5
26	14457.5
27	17706.0
28	19962.0
29	22218.0
30	26000.0
31	29782.0
32	34848.5
33	39915.0
34	46379.5
35	52844.0
36	57019.5
37	61195.0
38	66914.0
39	72633.0
40	94430.0
41	116227.0
42	139144.0
43	162061.0
44	168309.0
45	174557.0
46	176386.0
47	178215.0
48	178240.5
49	178266.0
50	164863.0
51	151460.0
52	137452.5
53	123445.0
54	109158.0
55	94871.0
56	84306.0
57	73741.0
58	68887.0
59	64033.0
60	59260.0
61	54487.0
62	47538.5
63	40590.0
64	33136.5
65	25683.0
66	20314.5
67	14946.0
68	12223.0
69	9500.0
70	7526.5
71	5553.0
72	4572.0
73	3591.0
74	2651.5
75	1216.0
76	720.0
77	601.0
78	482.0
79	374.5
80	267.0
81	238.0
82	209.0
83	135.0
84	61.0
85	46.5
86	32.0
87	22.5
88	13.0
89	14.5
90	16.0
91	13.0
92	10.0
93	9.0
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1871538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.820634980009626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57830226271716	15.376512115181715
2	9.217887683163697	3.6540877410942847
3	3.2676360391290524	1.9429986353730413
4	1.5761339705813049	1.249599044419411
5	0.9181686772322317	0.9099343100749169
6	0.5755946047481957	0.684519033430614
7	0.4312682575463027	0.5983607497903033
8	0.32123014655859844	0.5093588383610379
9	0.2521549656872701	0.44980843799558085
>10	2.743307145415783	13.573734863950374
>50	1.9268159061133956	28.572328608795992
>100	1.185224978651989	30.84011025638988
>500	0.0038197858421225683	0.5237549827203336
>1k	0.002182734766927182	0.6909131295028378
>5k	2.7284184586589774E-4	0.42397925291971256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7840	0.4189068028541232	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3536	0.18893551720563515	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.343199015996469E-5	0.0	0.0	0.10152078130393291	0.0
2	5.343199015996469E-5	0.0	0.0	0.3909618720004617	0.0
3	5.343199015996469E-5	0.0	0.0	0.5754091020326598	0.0
4	5.343199015996469E-5	0.0	0.0	0.9933541290639036	0.0
5	5.343199015996469E-5	0.0	0.0	1.6621623498961817	0.0
6	5.343199015996469E-5	0.0	0.0	2.4371933671664694	0.0
7	5.343199015996469E-5	0.0	0.0	2.8330709822616478	0.0
8	5.343199015996469E-5	0.0	0.0	3.7525286689343202	0.0
9	5.343199015996469E-5	0.0	0.0	4.075738777411947	0.0
10	5.343199015996469E-5	0.0	0.0	4.6783981944261885	0.0
11	5.343199015996469E-5	0.0	0.0	5.41677486644674	0.0
12	5.343199015996469E-5	0.0	0.0	5.987909409266603	0.0
13	5.343199015996469E-5	0.0	0.0	6.216331167200452	0.0
14	5.343199015996469E-5	0.0	0.0	6.303906199072634	0.0
15	5.343199015996469E-5	0.0	0.0	6.442348485577103	0.0
16	5.343199015996469E-5	0.0	0.0	6.777580791840721	0.0
17	5.343199015996469E-5	0.0	0.0	7.158550881681269	0.0
18	5.343199015996469E-5	0.0	0.0	7.658834605549019	0.0
19	5.343199015996469E-5	0.0	0.0	7.895698617928143	0.0
20	5.343199015996469E-5	0.0	0.0	8.14244754848686	0.0
21	5.343199015996469E-5	0.0	0.0	8.461543393722168	0.0
22	5.343199015996469E-5	0.0	0.0	8.770166568886125	0.0
23	5.343199015996469E-5	0.0	0.0	9.105185147189104	0.0
24	5.343199015996469E-5	0.0	0.0	9.353911061383739	0.0
25	5.343199015996469E-5	0.0	0.0	9.569081685757917	0.0
26	5.343199015996469E-5	0.0	0.0	9.765230521635146	0.0
27	5.343199015996469E-5	0.0	0.0	9.965066164833415	0.0
28	5.343199015996469E-5	0.0	0.0	10.16971068714608	0.0
29	5.343199015996469E-5	0.0	0.0	10.39749126119801	0.0
30	1.0686398031992938E-4	0.0	0.0	10.69879425371005	0.0
31	1.602959704798941E-4	0.0	0.0	10.965045860677154	0.0
32	1.602959704798941E-4	0.0	0.0	11.18310181251997	0.0
33	1.602959704798941E-4	0.0	0.0	11.41125641050302	0.0
34	1.602959704798941E-4	0.0	0.0	11.643952727649665	0.0
35	1.602959704798941E-4	0.0	0.0	11.948728799522105	0.0
36	1.602959704798941E-4	0.0	0.0	12.188050683448585	0.0
37	1.602959704798941E-4	0.0	0.0	12.434959909977783	0.0
38	1.602959704798941E-4	0.0	0.0	12.69602861389937	0.0
39	1.602959704798941E-4	0.0	0.0	13.010636171961243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	25	3.8917482E-5	45.000004	9
GACCGAC	25	3.8917482E-5	45.000004	9
GCGGATA	25	3.8917482E-5	45.000004	30
ACGTACG	45	3.8562575E-10	45.000004	1
AACCGGT	20	7.0344255E-4	45.0	37
ATCCGAC	20	7.0344255E-4	45.0	32
CCGTATT	20	7.0344255E-4	45.0	19
CGACTCC	20	7.0344255E-4	45.0	28
TAATGCG	75	0.0	45.0	1
GCGTAAG	140	0.0	45.0	1
AGTCGAC	20	7.0344255E-4	45.0	38
CGACGGT	305	0.0	42.786884	28
TATAGCG	85	0.0	42.352943	1
ATAACGG	125	0.0	41.4	2
CGGTCTA	310	0.0	41.370968	31
ACGCTAG	60	3.6379788E-12	41.250004	1
CGTTTTT	3775	0.0	41.125824	1
TACACGG	215	0.0	40.813953	2
CGCATCG	160	0.0	40.78125	21
TAAGGGA	2015	0.0	39.528534	4
>>END_MODULE