FastQCFastQC Report
Thu 26 May 2016
SRR1547870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547870_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1199157
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90240.7525286513775928No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16110.13434437692478968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG207.033408E-445.00000427
TACGGGA3550.043.098594
CGTTTTT39750.041.433961
TAACGCC1150.041.08695612
CGTAAGG2050.040.6097562
TCGCAAG451.9284926E-840.01
ACGGGAT4900.039.948985
GCGCGAC2650.039.9056639
TCACGAC3000.039.75000425
TAGACGG1700.039.7058832
CGGGATA2100.039.6428576
GCCGACC1500.039.00000414
CGACGGT2950.038.89830428
CGCATCG1050.038.5714321
TCGATAG356.248514E-638.571431
CGGTCTA3000.038.25000431
TCGTTTA1000.038.2538
CTACGGG2650.038.2075463
CACAACG1650.038.18181612
GCGTAAG1300.038.076921
Produced by FastQC (version 0.11.5)