##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547870_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1199157
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.488987680512228	31.0	31.0	33.0	30.0	34.0
2	31.895693391274037	31.0	31.0	34.0	30.0	34.0
3	31.92392489056896	33.0	31.0	34.0	30.0	34.0
4	35.72434968898985	37.0	35.0	37.0	35.0	37.0
5	35.64877576497489	37.0	35.0	37.0	33.0	37.0
6	35.697314863691744	37.0	35.0	37.0	33.0	37.0
7	36.018341218039005	37.0	35.0	37.0	35.0	37.0
8	36.038326090745414	37.0	35.0	37.0	35.0	37.0
9	37.76298099414839	39.0	38.0	39.0	35.0	39.0
10	37.33152289483362	39.0	37.0	39.0	34.0	39.0
11	37.06716051359413	39.0	37.0	39.0	33.0	39.0
12	36.4722667674041	39.0	35.0	39.0	33.0	39.0
13	36.25581304199534	39.0	35.0	39.0	32.0	39.0
14	37.18929964967056	39.0	35.0	41.0	32.0	41.0
15	37.41650509482912	39.0	35.0	41.0	32.0	41.0
16	37.54666236364379	40.0	35.0	41.0	33.0	41.0
17	37.49262106629907	39.0	35.0	41.0	33.0	41.0
18	37.387349613103204	39.0	35.0	41.0	32.0	41.0
19	37.30957414250177	39.0	35.0	41.0	32.0	41.0
20	37.13504153334384	39.0	35.0	41.0	32.0	41.0
21	37.01577358094061	39.0	35.0	41.0	32.0	41.0
22	36.95061864292999	39.0	35.0	41.0	32.0	41.0
23	36.87449433226842	38.0	35.0	41.0	32.0	41.0
24	36.900979604839065	38.0	35.0	41.0	32.0	41.0
25	36.85902763357926	38.0	35.0	41.0	32.0	41.0
26	36.765262596974374	38.0	35.0	41.0	31.0	41.0
27	36.67697974493748	38.0	35.0	41.0	31.0	41.0
28	36.59305662227715	38.0	35.0	41.0	31.0	41.0
29	36.580444428877954	38.0	35.0	41.0	31.0	41.0
30	36.49910061818427	38.0	35.0	40.0	31.0	41.0
31	36.31294651159106	38.0	35.0	40.0	30.0	41.0
32	36.172301875400805	38.0	35.0	40.0	30.0	41.0
33	36.05660309700898	38.0	35.0	40.0	30.0	41.0
34	35.857705871708205	38.0	35.0	40.0	29.0	41.0
35	35.76242977358261	38.0	35.0	40.0	29.0	41.0
36	35.62187103106599	38.0	35.0	40.0	29.0	41.0
37	35.50075427988162	38.0	34.0	40.0	28.0	41.0
38	35.37684389950607	38.0	34.0	40.0	27.0	41.0
39	35.29367714152526	38.0	34.0	40.0	27.0	41.0
40	35.12167797878009	38.0	34.0	40.0	26.0	41.0
41	35.03540820759917	38.0	34.0	40.0	25.0	41.0
42	34.96441917113439	37.0	34.0	40.0	25.0	41.0
43	34.913791938837036	37.0	34.0	40.0	25.0	41.0
44	34.73361202911712	37.0	34.0	40.0	24.0	41.0
45	34.61046635261271	37.0	34.0	40.0	24.0	41.0
46	34.564261393629025	37.0	34.0	40.0	24.0	41.0
47	34.47744623931645	37.0	34.0	40.0	24.0	41.0
48	34.35688571221283	36.0	33.0	40.0	23.0	41.0
49	34.235724763312895	36.0	33.0	40.0	24.0	41.0
50	34.14539380581525	36.0	33.0	40.0	24.0	41.0
51	33.816723748433276	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	5.0
13	11.0
14	17.0
15	43.0
16	105.0
17	221.0
18	477.0
19	983.0
20	1540.0
21	2743.0
22	4250.0
23	6209.0
24	9129.0
25	13325.0
26	17582.0
27	20274.0
28	22084.0
29	24697.0
30	28458.0
31	34056.0
32	42923.0
33	56252.0
34	96347.0
35	139240.0
36	86062.0
37	111916.0
38	174173.0
39	305989.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.84690828640453	24.5914421547804	28.52920843559267	14.032441123222398
2	31.20166917259375	26.41005306227625	28.87186581907123	13.516411946058774
3	29.24237610254537	25.39225472561141	31.39889105429898	13.966478117544243
4	26.59785165745603	27.533925916289526	30.6203441250812	15.24787830117324
5	23.538619213330698	32.055018650602044	29.702532695885527	14.703829440181728
6	22.202930892285163	40.346927049585666	26.933754295726082	10.516387762403088
7	87.36295580978971	3.535150109618674	7.310969289259038	1.7909247913325779
8	87.25337883196279	3.25903947523135	7.038694683014818	2.448887009791045
9	81.81380753312536	5.86553720655427	9.091136523407693	3.2295187369126808
10	43.908679180457604	31.52172734679446	14.337905712096081	10.231687760651859
11	35.64754239853497	21.58733176723315	26.011439703058066	16.75368613117382
12	33.05638877978447	20.796609618256827	29.065168280717202	17.081833321241504
13	24.480947865875777	29.982562750332107	28.97402091636041	16.562468467431703
14	18.285178671349954	33.62612235095154	31.041890261241857	17.046808716456642
15	16.854340173972215	25.46397177350422	40.49736606632826	17.184321986195304
16	21.696074825898528	23.856759373459855	36.823285024396306	17.62388077624531
17	22.02897535518702	23.63251851092059	27.910440417726782	26.428065716165605
18	23.353155591803244	25.123649363677984	32.04434448533428	19.478850559184494
19	26.60594067332301	26.59293153440292	26.765886368507207	20.035241423766863
20	27.18509753101554	27.299094280398645	26.770723099644165	18.74508508894165
21	23.832075366278143	26.454167385921945	30.666793422379225	19.04696382542069
22	23.117073077170044	23.50759742052125	29.63473506805197	23.74059443425673
23	20.067097135737853	27.709048940213833	29.464532167180778	22.759321756867532
24	20.898597931713695	26.59785165745603	33.26620284082902	19.23734757000126
25	21.054957774503254	26.575252448178176	31.04797787112113	21.32181190619744
26	19.586926482520635	30.2270678484969	27.485892172584574	22.700113496397886
27	19.56215908342277	29.508896666574934	30.756356340329084	20.17258790967321
28	17.03621794310503	28.119837519190565	33.976034831135536	20.867909706568867
29	18.179104154001518	27.05275456007846	33.27095618004982	21.497185105870205
30	21.12242183467219	27.549603596526563	31.00419711514005	20.323777453661197
31	25.094128625359314	26.79407283616741	27.469714140850616	20.64208439762266
32	25.915205431815853	27.204944807060293	27.294340941177843	19.585508819946014
33	24.334678444940906	27.031322837626764	26.760882853537943	21.873115863894384
34	19.48627243972224	28.08548004973494	29.35095237737844	23.07729513316438
35	20.011391335746694	28.16486915391396	29.32118146331131	22.50255804702804
36	26.012690581800378	26.300476084449325	27.458372840253613	20.22846049349668
37	20.523667876683373	30.9684219831098	27.80269806205526	20.70521207815157
38	20.925533520631575	30.733256779554303	25.689963866282728	22.651245833531387
39	20.471881496751468	28.437227152074335	27.585462120472965	23.505429230701232
40	23.435463413047668	26.304812464089355	27.56694911508668	22.692775007776298
41	19.405132105303977	25.66152722287407	28.636367047851113	26.29697362397084
42	21.917480363288544	26.6641482307988	27.61464929112702	23.803722114785636
43	21.474919464256974	26.3122343446271	27.939794372213143	24.27305181890278
44	20.758916472155022	28.481174691887716	28.042699996747718	22.717208839209544
45	19.646635094487213	30.195295528442063	26.195902621591667	23.962166755479057
46	22.212354178810614	28.16311792367471	27.80703444169529	21.81749345581938
47	20.755330619760382	27.500735933660064	28.310471439519596	23.433462007059962
48	21.645872892373557	26.70134102540368	29.1940087911758	22.45877729104696
49	21.889043719879883	25.027498484351923	29.4752897243647	23.608168071403494
50	19.87696356690575	28.391945341602476	28.720676275083246	23.010414816408527
51	19.54931672833499	29.29841547020115	26.922829954709847	24.229437846754013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	576.0
1	1120.5
2	1665.0
3	7623.5
4	13582.0
5	9679.0
6	5776.0
7	5335.5
8	4895.0
9	5298.0
10	5701.0
11	5858.5
12	6016.0
13	6118.0
14	6220.0
15	5882.0
16	5544.0
17	5315.0
18	5086.0
19	5096.0
20	5106.0
21	5809.5
22	6513.0
23	6720.5
24	6928.0
25	7744.0
26	10471.0
27	12382.0
28	13912.0
29	15442.0
30	18393.0
31	21344.0
32	23461.5
33	25579.0
34	28696.0
35	31813.0
36	34386.5
37	36960.0
38	42294.0
39	47628.0
40	58020.0
41	68412.0
42	78549.0
43	88686.0
44	92642.5
45	96599.0
46	99786.5
47	102974.0
48	104162.5
49	105351.0
50	100169.5
51	94988.0
52	85071.5
53	75155.0
54	69521.0
55	63887.0
56	60070.5
57	56254.0
58	53076.0
59	49898.0
60	43992.0
61	38086.0
62	33470.0
63	28854.0
64	24708.0
65	20562.0
66	16973.5
67	13385.0
68	11393.5
69	9402.0
70	7620.5
71	5839.0
72	4598.5
73	3358.0
74	2636.5
75	1491.0
76	1067.0
77	832.5
78	598.0
79	431.5
80	265.0
81	204.0
82	143.0
83	103.0
84	63.0
85	49.0
86	35.0
87	28.0
88	21.0
89	21.0
90	21.0
91	20.5
92	20.0
93	11.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1199157.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.24933198179287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47102128480834	19.30842203406875
2	7.642313821944041	3.8592663759861883
3	2.7796702357294802	2.105544497455263
4	1.5254167004679868	1.5406301072274917
5	0.935909964256214	1.1815550696286525
6	0.6888469704017884	1.0435755504196202
7	0.5654850475676687	0.9994683787743202
8	0.45127497874593225	0.9115513402746053
9	0.3808809495655621	0.8655290587009247
>10	7.588032885836157	50.65565912680109
>50	0.9143940174418451	13.920135988708118
>100	0.053080881025675666	2.2076305188041188
>500	0.0030045781712616797	0.5045771777607173
>1k	3.3384201902907556E-4	0.13579582916346736
>5k	3.3384201902907556E-4	0.7606589462266478
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9024	0.7525286513775928	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1611	0.13434437692478968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.339191615443182E-5	0.0	0.0	0.07079973681511262	0.0
2	8.339191615443182E-5	0.0	0.0	0.23541537930396103	0.0
3	8.339191615443182E-5	0.0	0.0	0.3681753098218165	0.0
4	8.339191615443182E-5	0.0	0.0	0.6081772445142712	0.0
5	8.339191615443182E-5	0.0	0.0	1.1205371773671005	0.0
6	8.339191615443182E-5	0.0	0.0	1.6940233847611281	0.0
7	8.339191615443182E-5	0.0	0.0	2.019001682014949	0.0
8	8.339191615443182E-5	0.0	0.0	2.73291987621304	0.0
9	8.339191615443182E-5	0.0	0.0	3.0413031821521286	0.0
10	8.339191615443182E-5	0.0	0.0	3.563253185362717	0.0
11	8.339191615443182E-5	0.0	0.0	4.1879420292755665	0.0
12	8.339191615443182E-5	0.0	0.0	4.667695722912012	0.0
13	8.339191615443182E-5	0.0	0.0	4.86691901060495	0.0
14	8.339191615443182E-5	0.0	0.0	4.95873351029098	0.0
15	8.339191615443182E-5	0.0	0.0	5.091159873144218	0.0
16	8.339191615443182E-5	0.0	0.0	5.375943266811602	0.0
17	8.339191615443182E-5	0.0	0.0	5.735946168850284	0.0
18	8.339191615443182E-5	0.0	0.0	6.173837120577206	0.0
19	8.339191615443182E-5	0.0	0.0	6.3910730621595	0.0
20	8.339191615443182E-5	0.0	0.0	6.638079917808928	0.0
21	8.339191615443182E-5	0.0	0.0	6.943127547101839	0.0
22	8.339191615443182E-5	0.0	0.0	7.268272628187969	0.0
23	8.339191615443182E-5	0.0	0.0	7.607677726936506	0.0
24	8.339191615443182E-5	0.0	0.0	7.847929837377424	0.0
25	8.339191615443182E-5	0.0	0.0	8.059578520577372	0.0
26	8.339191615443182E-5	0.0	0.0	8.264722634317275	0.0
27	8.339191615443182E-5	0.0	0.0	8.515148558529033	0.0
28	8.339191615443182E-5	0.0	0.0	8.747061477354508	0.0
29	8.339191615443182E-5	0.0	0.0	9.01541666353947	0.0
30	8.339191615443182E-5	0.0	0.0	9.35732351977264	0.0
31	8.339191615443182E-5	0.0	0.0	9.623927475718359	0.0
32	8.339191615443182E-5	0.0	0.0	9.869600060709315	0.0
33	8.339191615443182E-5	0.0	0.0	10.120693120250309	0.0
34	8.339191615443182E-5	0.0	0.0	10.389131698351425	0.0
35	8.339191615443182E-5	0.0	0.0	10.707105074648274	0.0
36	8.339191615443182E-5	0.0	0.0	10.968455339876263	0.0
37	8.339191615443182E-5	0.0	0.0	11.248151826658228	0.0
38	8.339191615443182E-5	0.0	0.0	11.585472127502904	0.0
39	8.339191615443182E-5	0.0	0.0	12.164212025614662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGCG	20	7.033408E-4	45.000004	27
TACGGGA	355	0.0	43.09859	4
CGTTTTT	3975	0.0	41.43396	1
TAACGCC	115	0.0	41.086956	12
CGTAAGG	205	0.0	40.609756	2
TCGCAAG	45	1.9284926E-8	40.0	1
ACGGGAT	490	0.0	39.94898	5
GCGCGAC	265	0.0	39.905663	9
TCACGAC	300	0.0	39.750004	25
TAGACGG	170	0.0	39.705883	2
CGGGATA	210	0.0	39.642857	6
GCCGACC	150	0.0	39.000004	14
CGACGGT	295	0.0	38.898304	28
CGCATCG	105	0.0	38.57143	21
TCGATAG	35	6.248514E-6	38.57143	1
CGGTCTA	300	0.0	38.250004	31
TCGTTTA	100	0.0	38.25	38
CTACGGG	265	0.0	38.207546	3
CACAACG	165	0.0	38.181816	12
GCGTAAG	130	0.0	38.07692	1
>>END_MODULE