FastQCFastQC Report
Thu 26 May 2016
SRR1547869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547869_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2878722
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295781.0274698286253414No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.10435186169418235No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT29280.101711801278484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT147400.042.496611
TACGGGA12500.039.064
TAACGGG7250.038.4827583
ACGGGAT12450.038.132535
CGGTCTA3350.037.61194231
AGGGCGA20650.037.59086
GGGCGAT38850.037.4131287
GACCGAT10250.037.317079
TAGGGAC32950.037.2837645
TAGGGAT48300.036.8012435
GGCGATA9550.036.753928
TAGGGCG8400.036.696435
GTAGGGA39300.036.583974
TAAGGGA34600.036.5462464
AGGGATC34650.036.4935076
TATCGCG1050.036.428571
TTAGGGA39600.036.3068164
CGTAAGG4650.036.2903252
GCGCGAC3550.036.1267629
AAGGGAT43800.036.1130145
Produced by FastQC (version 0.11.5)