##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547869_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2878722
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446465827544305	31.0	31.0	33.0	30.0	34.0
2	31.84993375532615	31.0	31.0	34.0	30.0	34.0
3	31.787114212487346	31.0	31.0	34.0	30.0	34.0
4	35.64716391509844	37.0	35.0	37.0	33.0	37.0
5	35.60992030491308	37.0	35.0	37.0	33.0	37.0
6	35.669334517192006	37.0	35.0	37.0	33.0	37.0
7	35.99030472549972	37.0	35.0	37.0	35.0	37.0
8	36.016245055965804	37.0	35.0	37.0	35.0	37.0
9	37.74062969609431	39.0	38.0	39.0	35.0	39.0
10	37.3194202149426	39.0	37.0	39.0	34.0	39.0
11	37.03914132729732	39.0	37.0	39.0	33.0	39.0
12	36.61385156329788	39.0	35.0	39.0	33.0	39.0
13	36.489489780534555	39.0	35.0	39.0	32.0	39.0
14	37.53390844965231	40.0	36.0	41.0	32.0	41.0
15	37.68025985142018	40.0	36.0	41.0	33.0	41.0
16	37.75583957047606	40.0	36.0	41.0	33.0	41.0
17	37.66994346796947	40.0	36.0	41.0	33.0	41.0
18	37.5214275640371	39.0	36.0	41.0	32.0	41.0
19	37.42149155076454	39.0	36.0	41.0	32.0	41.0
20	37.21063687289012	39.0	35.0	41.0	32.0	41.0
21	37.10320447754246	39.0	35.0	41.0	32.0	41.0
22	37.03271382231421	39.0	35.0	41.0	32.0	41.0
23	36.933012982844474	39.0	35.0	41.0	32.0	41.0
24	36.98744998648706	39.0	35.0	41.0	32.0	41.0
25	36.97110870726663	39.0	35.0	41.0	32.0	41.0
26	36.885250816160784	39.0	35.0	41.0	32.0	41.0
27	36.797900596167324	39.0	35.0	41.0	31.0	41.0
28	36.70628042582785	38.0	35.0	41.0	31.0	41.0
29	36.671456639439306	38.0	35.0	41.0	31.0	41.0
30	36.54019353032353	38.0	35.0	41.0	31.0	41.0
31	36.33011732289537	38.0	35.0	41.0	30.0	41.0
32	36.14352375811211	38.0	35.0	41.0	30.0	41.0
33	35.90860458217223	38.0	35.0	41.0	29.0	41.0
34	35.646749842464814	38.0	35.0	41.0	27.0	41.0
35	35.460639478212904	38.0	34.0	40.0	26.0	41.0
36	35.328424905218355	38.0	34.0	40.0	25.0	41.0
37	35.250457668368114	38.0	34.0	40.0	25.0	41.0
38	35.12902148939703	38.0	34.0	40.0	24.0	41.0
39	35.018839957453345	38.0	34.0	40.0	24.0	41.0
40	34.86426059897413	38.0	34.0	40.0	23.0	41.0
41	34.76211978787809	38.0	34.0	40.0	23.0	41.0
42	34.672956610607066	38.0	34.0	40.0	23.0	41.0
43	34.605603111380674	38.0	34.0	40.0	23.0	41.0
44	34.410356053832224	37.0	33.0	40.0	23.0	41.0
45	34.284063900578104	37.0	33.0	40.0	23.0	41.0
46	34.23307078627252	37.0	33.0	40.0	23.0	41.0
47	34.14573202969929	37.0	33.0	40.0	22.0	41.0
48	34.01153636926386	37.0	33.0	40.0	22.0	41.0
49	33.85641336676483	36.0	33.0	40.0	21.0	41.0
50	33.78432894874879	36.0	33.0	40.0	20.0	41.0
51	33.454644456811046	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	1.0
11	8.0
12	6.0
13	11.0
14	26.0
15	86.0
16	208.0
17	506.0
18	1216.0
19	2273.0
20	4139.0
21	6757.0
22	10878.0
23	16038.0
24	24576.0
25	37196.0
26	50463.0
27	58275.0
28	60586.0
29	64120.0
30	71473.0
31	83165.0
32	100536.0
33	128963.0
34	211887.0
35	279527.0
36	207386.0
37	272933.0
38	434909.0
39	750493.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.33735108843438	24.663062289446497	28.9330821107422	16.066504511376923
2	33.267922362770705	25.47939676009007	28.0837468849024	13.168933992236834
3	28.69064814177958	25.04323098930706	32.95062878596822	13.315492082945141
4	25.51719130919901	26.877412963113496	32.97782835577732	14.627567371910175
5	23.406949333766857	30.605629859361205	30.99100920477906	14.996411602092873
6	21.51402601571114	38.941898523025145	28.404896339417284	11.13917912184643
7	85.31431656130741	3.1764790070038025	9.37610509107861	2.1330993406101735
8	85.92031463962132	2.445043321307163	9.039045798795438	2.595596240276067
9	80.42874581150942	4.959944030719187	11.081340956160407	3.5299692016109927
10	41.63594122669713	32.65723470345521	15.195145623648271	10.511678446199388
11	30.491864098026834	21.953735025473108	30.12163036236219	17.43277051413787
12	29.063244036763535	20.309290025226474	31.610485486267866	19.01698045174213
13	23.873510536967444	24.447549989196595	32.58272247198583	19.09621700185013
14	18.439814612178598	28.24142101946628	33.119210538565376	20.199553829789746
15	18.110536550594325	25.202086203530595	38.49166400923743	18.195713236637648
16	22.498178010936797	23.753735164423656	35.5313920552245	18.21669476941504
17	22.111791274044524	23.391803724013645	30.365870688451334	24.1305343134905
18	23.473402433440953	23.956741915336043	32.5302339024053	20.039621748817705
19	25.114582095805016	26.176859036753115	28.974280948281912	19.734277919159958
20	25.45025188260624	26.442810385997674	28.748034718183973	19.35890301321211
21	24.540577381212913	25.718739079355352	31.45138710858499	18.289296430846743
22	23.608879217930735	22.68430227024353	31.5887397254754	22.11807878635033
23	21.042254166953253	26.412866542861728	30.638908515653824	21.9059707745312
24	21.162203227682284	25.14042689776922	33.4964960145509	20.200873859997596
25	21.987083157039823	25.673232774821603	30.43454699689654	21.90513707124203
26	20.02423297560515	27.502377791255984	29.83490590616253	22.638483326976345
27	19.74310127897032	26.35784212577665	32.06402007557521	21.83503651967783
28	19.244789875507255	26.027904049088452	33.71489153867584	21.012414536728453
29	20.2202227238337	25.02714746335353	33.06606195388092	21.686567858931845
30	22.423144714911686	24.499204855487953	32.51046818692461	20.567182242675745
31	24.38863495676206	23.81153859247263	30.806552352050666	20.993274098714636
32	25.83670809477261	24.002908234973713	30.303725055771274	19.8566586144824
33	24.000546075654405	24.46245243549047	30.835141427341718	20.701860061513408
34	21.643979515910186	24.55207553907602	32.41841344874566	21.385531496268136
35	20.646140891687352	25.792695508631958	31.799076117805054	21.76208748187564
36	23.785172725952698	25.292959862049898	30.340234312309423	20.581633099687988
37	21.468415498266243	26.725540013936737	29.84491034563254	21.961134142164475
38	21.630153936364817	27.267551364807023	29.108020850919264	21.9942738479089
39	21.344992673832348	26.148513124921408	28.813306738198406	23.69318746304784
40	23.66008249494046	24.515878921271312	29.10857665311204	22.71546193067618
41	20.41923464648549	24.716454037590292	29.778526721232545	25.085784594691674
42	21.89732110290608	25.387168333725867	28.81587732334001	23.899633240028038
43	21.417802761086342	25.366742603141258	29.55162047603068	23.663834159741718
44	21.505793195730604	26.62612089670347	29.326694276140593	22.541391631425334
45	20.588545889460672	26.984717523956814	27.79796034490305	24.628776241679468
46	22.313095880741525	26.12193883257918	28.656049455279113	22.908915831400183
47	21.174187712464075	25.708838852796482	29.94804639003002	23.168927044709424
48	21.796130366183327	24.704122176438016	30.928203556995083	22.571543900383574
49	22.611526920626584	23.719205953197285	30.249221703241925	23.420045422934205
50	20.523794934001963	25.340689375354756	30.851607067302783	23.283908623340498
51	19.916233662020854	25.98225879400651	29.90111584237728	24.20039170159536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1234.0
1	2319.0
2	3404.0
3	25834.5
4	48265.0
5	34522.0
6	20779.0
7	18764.5
8	16750.0
9	16959.0
10	17168.0
11	17108.5
12	17049.0
13	16760.5
14	16472.0
15	15524.5
16	14577.0
17	13725.0
18	12873.0
19	12530.0
20	12187.0
21	12877.0
22	13567.0
23	14774.0
24	15981.0
25	18291.0
26	23006.0
27	25411.0
28	29699.0
29	33987.0
30	40039.0
31	46091.0
32	52271.0
33	58451.0
34	66909.0
35	75367.0
36	80650.5
37	85934.0
38	97168.0
39	108402.0
40	125904.5
41	143407.0
42	162014.0
43	180621.0
44	190666.5
45	200712.0
46	217849.5
47	234987.0
48	242444.5
49	249902.0
50	240725.5
51	231549.0
52	213216.5
53	194884.0
54	176376.0
55	157868.0
56	152250.0
57	146632.0
58	140323.5
59	134015.0
60	123132.5
61	112250.0
62	97824.5
63	83399.0
64	70228.5
65	57058.0
66	45962.0
67	34866.0
68	28033.0
69	21200.0
70	17040.5
71	12881.0
72	10489.0
73	8097.0
74	6339.5
75	3680.5
76	2779.0
77	2136.5
78	1494.0
79	970.0
80	446.0
81	372.0
82	298.0
83	205.0
84	112.0
85	84.0
86	56.0
87	46.0
88	36.0
89	28.5
90	21.0
91	14.5
92	8.0
93	8.0
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2878722.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.718805510567282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3830376394843	18.354332195878357
2	7.42019348908366	3.5199625243670587
3	2.651701320289587	1.8868556366418956
4	1.422363803616792	1.3494708169302962
5	0.9263484239466451	1.09859390513055
6	0.6520274287877869	0.9279187062583664
7	0.5272182344417969	0.8753490735044752
8	0.4356082091633394	0.8265685113561407
9	0.37473398231075494	0.7999418200166241
>10	6.449365955328255	39.96623916430643
>50	1.6816956889098171	25.501074024181534
>100	0.07049123330044468	2.5121782247827693
>500	0.0028307781542513183	0.4426822675358923
>1k	0.002234824858619462	0.8935983475038005
>5k	0.0	0.0
>10k+	1.489883239079641E-4	1.0452347816058611
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29578	1.0274698286253414	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.10435186169418235	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	2928	0.101711801278484	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04752108748257039	0.0
2	0.0	0.0	0.0	0.21137852144111172	0.0
3	0.0	0.0	0.0	0.4338383491007468	0.0
4	0.0	0.0	0.0	0.672138539254572	0.0
5	0.0	0.0	0.0	1.2533339447157454	0.0
6	0.0	0.0	0.0	2.295080942168087	0.0
7	0.0	0.0	0.0	2.855815879407598	0.0
8	0.0	0.0	0.0	3.895270192814728	0.0
9	0.0	0.0	0.0	4.568311910632565	0.0
10	0.0	0.0	0.0	5.410838559610827	0.0
11	0.0	0.0	0.0	6.118270538106841	0.0
12	0.0	0.0	0.0	6.633429695538506	0.0
13	0.0	0.0	0.0	6.882845929547903	0.0
14	0.0	0.0	0.0	7.046217036587763	0.0
15	0.0	0.0	0.0	7.180373790869698	0.0
16	0.0	0.0	0.0	7.403042044351626	0.0
17	0.0	0.0	0.0	7.654438323672797	0.0
18	0.0	0.0	0.0	7.986182757487524	0.0
19	0.0	0.0	0.0	8.185819957606189	0.0
20	0.0	0.0	0.0	8.401749109500674	0.0
21	0.0	0.0	0.0	8.641265116951203	0.0
22	0.0	0.0	0.0	8.905826960713817	0.0
23	0.0	0.0	0.0	9.177058430789774	0.0
24	0.0	0.0	0.0	9.393126533232456	0.0
25	0.0	0.0	0.0	9.583836160629613	0.0
26	0.0	0.0	0.0	9.768466701543254	0.0
27	0.0	0.0	0.0	9.978525192776516	0.0
28	0.0	0.0	0.0	10.178127655258132	0.0
29	0.0	0.0	0.0	10.39867691288009	0.0
30	0.0	0.0	0.0	10.692592059948824	0.0
31	0.0	0.0	0.0	10.92074886008444	0.0
32	0.0	0.0	0.0	11.159222738423509	0.0
33	0.0	0.0	0.0	11.391165940997428	0.0
34	0.0	0.0	0.0	11.630299834440422	0.0
35	0.0	0.0	0.0	11.895209054573522	0.0
36	0.0	0.0	0.0	12.127916485162514	0.0
37	0.0	0.0	0.0	12.372087335977563	0.0
38	0.0	0.0	0.0	12.6607223622149	0.0
39	0.0	0.0	0.0	13.074239193642178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14740	0.0	42.49661	1
TACGGGA	1250	0.0	39.06	4
TAACGGG	725	0.0	38.482758	3
ACGGGAT	1245	0.0	38.13253	5
CGGTCTA	335	0.0	37.611942	31
AGGGCGA	2065	0.0	37.5908	6
GGGCGAT	3885	0.0	37.413128	7
GACCGAT	1025	0.0	37.31707	9
TAGGGAC	3295	0.0	37.283764	5
TAGGGAT	4830	0.0	36.801243	5
GGCGATA	955	0.0	36.75392	8
TAGGGCG	840	0.0	36.69643	5
GTAGGGA	3930	0.0	36.58397	4
TAAGGGA	3460	0.0	36.546246	4
AGGGATC	3465	0.0	36.493507	6
TATCGCG	105	0.0	36.42857	1
TTAGGGA	3960	0.0	36.306816	4
CGTAAGG	465	0.0	36.290325	2
GCGCGAC	355	0.0	36.126762	9
AAGGGAT	4380	0.0	36.113014	5
>>END_MODULE