FastQCFastQC Report
Thu 26 May 2016
SRR1547868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547868_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3348846
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170460.5090111638456949No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37360.11156081826396316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTC207.0352235E-445.031
CGTTTTT85500.041.7631571
CGGTCTA4800.041.24999631
CACGACG4950.040.45454426
CGACGGT5100.039.26470628
TACGGGA13150.039.1825074
ATTACGG3200.038.6718752
TAACGGG9450.038.571433
CGCATCG1350.038.33333621
TTACGGG10000.038.0249983
TAGTACG2150.037.674421
GGGCGAT44050.037.5425647
ATCGTAG601.5643309E-1037.4999961
AATACCG601.5643309E-1037.4999961
TACGGGT2950.037.3728834
TAAGGGA36300.037.314054
CGTTAGG4600.037.1739122
GTACGGG14250.036.947373
TCTAACG552.752131E-936.818181
AGTACGG5200.036.7788432
Produced by FastQC (version 0.11.5)