FastQCFastQC Report
Thu 26 May 2016
SRR1547867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547867_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2635520
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115140.43687773190869356No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27090.10278806459446334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT207.0349494E-445.037
CGTTAGG3350.042.3134352
CGTAAGG3700.041.9594572
CGACGGT3450.041.73913228
CGTTTTT59250.041.3924031
CGGTCTA3600.040.031
CTATGCG1250.039.61
TAGGGCG7250.039.4137955
CTACCGT403.4605E-739.37524
TAAGGGA35600.038.995794
TACGGGA12050.038.651454
TAGGGAC32900.038.5714265
TAGTCGG700.038.5714262
GGGCGAT33500.038.4179087
TTGCGAG2050.038.4146351
TATAGCG1300.038.0769271
GATCGAC301.1403223E-437.50000422
TCGCTAA301.1403223E-437.50000422
AGGGCGA18150.037.0661166
CGGGTAT1700.037.0588236
Produced by FastQC (version 0.11.5)