##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547867_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2635520
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48946317994172	31.0	31.0	33.0	30.0	34.0
2	31.908563774890723	31.0	31.0	34.0	30.0	34.0
3	31.999909315808644	33.0	31.0	34.0	30.0	34.0
4	35.77383362676056	37.0	35.0	37.0	35.0	37.0
5	35.6883776256678	37.0	35.0	37.0	33.0	37.0
6	35.72527357030112	37.0	35.0	37.0	33.0	37.0
7	36.02697304516756	37.0	35.0	37.0	35.0	37.0
8	36.047581881374455	37.0	35.0	37.0	35.0	37.0
9	37.7996801390238	39.0	38.0	39.0	35.0	39.0
10	37.33706365347256	39.0	37.0	39.0	34.0	39.0
11	37.0247283268577	39.0	37.0	39.0	33.0	39.0
12	36.52754788428849	39.0	35.0	39.0	33.0	39.0
13	36.358566810344826	39.0	35.0	39.0	32.0	39.0
14	37.399087466610005	40.0	35.0	41.0	32.0	41.0
15	37.586232697911605	40.0	35.0	41.0	33.0	41.0
16	37.67856817629917	40.0	35.0	41.0	33.0	41.0
17	37.61023023919378	39.0	35.0	41.0	33.0	41.0
18	37.51998922413793	39.0	36.0	41.0	33.0	41.0
19	37.45955219463332	39.0	36.0	41.0	32.0	41.0
20	37.33591283693541	39.0	35.0	41.0	32.0	41.0
21	37.229745932491504	39.0	35.0	41.0	32.0	41.0
22	37.153607637202526	39.0	35.0	41.0	32.0	41.0
23	37.04668831957261	39.0	35.0	41.0	32.0	41.0
24	37.085872996600294	39.0	35.0	41.0	32.0	41.0
25	37.060819876153474	39.0	35.0	41.0	32.0	41.0
26	36.97365301724138	39.0	35.0	41.0	32.0	41.0
27	36.89088225473531	39.0	35.0	41.0	32.0	41.0
28	36.82802445058281	39.0	35.0	41.0	31.0	41.0
29	36.84280635320544	39.0	35.0	41.0	31.0	41.0
30	36.76657471770277	38.0	35.0	41.0	31.0	41.0
31	36.632362493929094	38.0	35.0	41.0	31.0	41.0
32	36.51886952100534	38.0	35.0	41.0	31.0	41.0
33	36.43954210174842	38.0	35.0	41.0	30.0	41.0
34	36.31769366197183	38.0	35.0	41.0	30.0	41.0
35	36.25144335842642	38.0	35.0	41.0	30.0	41.0
36	36.14606681034483	38.0	35.0	41.0	30.0	41.0
37	36.05341640359398	38.0	35.0	41.0	30.0	41.0
38	35.95597339424478	38.0	35.0	40.0	30.0	41.0
39	35.87352628703254	38.0	35.0	40.0	29.0	41.0
40	35.72112486340457	38.0	35.0	40.0	29.0	41.0
41	35.67845852051967	38.0	35.0	40.0	29.0	41.0
42	35.61446583596406	38.0	35.0	40.0	28.0	41.0
43	35.5329365741865	38.0	35.0	40.0	28.0	41.0
44	35.373092217095675	38.0	34.0	40.0	27.0	41.0
45	35.21558819511899	38.0	34.0	40.0	27.0	41.0
46	35.15638128339	38.0	34.0	40.0	27.0	41.0
47	35.07152857880039	37.0	34.0	40.0	27.0	41.0
48	35.008841898373	37.0	34.0	40.0	26.0	41.0
49	34.87106643091307	37.0	34.0	40.0	26.0	41.0
50	34.78261253946091	37.0	34.0	40.0	26.0	41.0
51	34.431741743564835	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	3.0
12	11.0
13	14.0
14	29.0
15	43.0
16	132.0
17	321.0
18	732.0
19	1513.0
20	2712.0
21	4367.0
22	6808.0
23	10528.0
24	15875.0
25	23211.0
26	31450.0
27	37208.0
28	42407.0
29	49088.0
30	58831.0
31	71898.0
32	89710.0
33	117635.0
34	203141.0
35	301924.0
36	185778.0
37	244819.0
38	398503.0
39	736733.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.21412852112676	24.792450825643517	29.111105208839245	12.88231544439048
2	29.753445240407967	26.184244475473527	30.22139843370568	13.840911850412821
3	28.325985004856726	26.325468977659057	31.30239952646916	14.046146491015055
4	25.015404929577468	28.759827282661487	31.33924235065566	14.88552543710539
5	23.370568237008257	32.117191294317635	29.792071393880526	14.72016907479359
6	21.424045349684313	40.92573761534726	26.534953254006798	11.115263780961632
7	89.39541342884895	3.157441415735794	5.616462785332685	1.8306823700825643
8	90.14839576250607	2.298977051966974	5.274860369111219	2.277766816415736
9	84.95754158572122	5.091594827586207	7.122579225352113	2.8282843613404567
10	44.3015420106848	34.22865316901408	11.605641391452162	9.864163428848956
11	32.714378946090335	23.317599562894607	27.035954953861097	16.932066537153958
12	31.537305730937348	22.305389448761535	28.232796563865953	17.92450825643516
13	23.602552816901408	30.4456805488101	28.88185253763963	17.06991409664886
14	17.714454832442932	33.91144821515299	30.065565808644973	18.308531143759108
15	16.458383924235065	26.978471041767847	39.13379522826615	17.429349805730936
16	20.49777652986887	24.433053059737738	36.52607455075279	18.5430958596406
17	20.688630706653715	24.12647219524041	28.17971406022341	27.005183037882468
18	22.2472605026712	25.516216913550267	31.74045349684313	20.496069086935407
19	24.95355755220981	27.297611097620205	27.43276469159786	20.316066658572122
20	26.63789309130646	27.127170349684317	26.463392423506555	19.771544135502673
21	23.815338149587177	27.12910545167557	29.62034057795046	19.435215820786787
22	22.886906568722683	23.62376305245265	28.95314776590578	24.536182612918893
23	19.70373967945605	28.42213301359883	28.93702191597863	22.93710539096649
24	20.16820210053424	26.29261018698397	33.74586419378339	19.793323518698397
25	20.178105269548325	27.169287275376398	30.976581471588148	21.67602598348713
26	19.230474441476446	31.191719281204467	27.328876274890725	22.248930002428363
27	19.46788489558038	30.00216276104905	29.973477719766876	20.55647462360369
28	17.379454528897522	28.11551420592521	33.99503703254007	20.5099942326372
29	17.86501335599806	26.284680063137444	33.82782145458961	22.02248512627489
30	20.343651347741623	27.344926238465273	31.78913459203497	20.522287821758134
31	24.29736826129189	27.40954346770277	26.674128824672174	21.61895944633317
32	24.51876669499757	27.681254553181155	27.393531447304515	20.406447304516757
33	22.843158086449733	26.989512506070906	27.038307430791647	23.129021976687714
34	19.029717095677515	28.285613465274405	29.397841792132102	23.286827646915977
35	19.00456076979116	28.030559434191353	29.2462967459932	23.718583050024282
36	23.615149951432734	27.14424477901894	27.707966549295776	21.53263872025255
37	19.79415826857698	30.14611917192812	28.41052240165129	21.649200157843612
38	19.475473530840215	30.43604298203011	26.401431216610007	23.687052270519672
39	19.472551906265178	28.78540098348713	27.673817690626517	24.068229419621176
40	21.67234549538611	26.74724532540068	27.79967520641088	23.78073397280233
41	18.22748451918407	26.26650528169014	28.571439412336087	26.934570786789703
42	21.451478266148616	26.675267119961145	27.076098834385625	24.797155779504614
43	21.096443965517242	26.703193297717338	28.088764266634286	24.11159847013113
44	20.504530415250123	28.698169621175328	27.622025254978144	23.175274708596405
45	19.686323761534727	30.181937530354542	26.21949368625546	23.91224502185527
46	22.15938410636231	28.56066355026712	27.483115286546866	21.7968370568237
47	19.919029261777563	28.218567872753763	28.582139388052454	23.28026347741622
48	20.873831350169986	25.843666525012143	29.62201007770762	23.66049204711025
49	20.813805245264692	24.989110308402136	29.917966852841182	24.279117593491986
50	19.30784057795046	27.9870765541525	29.45718491986401	23.247897948033025
51	18.785666585721223	28.962443844099077	27.35471557795046	24.897173992229238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	868.0
1	1801.5
2	2735.0
3	10876.5
4	19018.0
5	14060.5
6	9103.0
7	8566.5
8	8030.0
9	8312.5
10	8595.0
11	8807.5
12	9020.0
13	9086.0
14	9152.0
15	8862.0
16	8572.0
17	8448.5
18	8325.0
19	8693.0
20	9061.0
21	9662.5
22	10264.0
23	11810.0
24	13356.0
25	16190.5
26	22710.5
27	26396.0
28	32403.5
29	38411.0
30	46240.5
31	54070.0
32	59133.5
33	64197.0
34	72695.0
35	81193.0
36	87552.0
37	93911.0
38	103498.5
39	113086.0
40	137836.5
41	162587.0
42	185470.0
43	208353.0
44	218847.5
45	229342.0
46	235025.0
47	240708.0
48	241744.0
49	242780.0
50	227661.0
51	212542.0
52	193747.0
53	174952.0
54	153681.0
55	132410.0
56	122042.0
57	111674.0
58	103179.0
59	94684.0
60	86131.0
61	77578.0
62	66059.0
63	54540.0
64	45672.5
65	36805.0
66	28804.0
67	20803.0
68	16463.0
69	12123.0
70	9761.5
71	7400.0
72	5971.0
73	4542.0
74	3520.5
75	1899.0
76	1299.0
77	1089.5
78	880.0
79	589.5
80	299.0
81	214.5
82	130.0
83	123.0
84	116.0
85	73.0
86	30.0
87	23.5
88	17.0
89	15.0
90	13.0
91	9.5
92	6.0
93	12.5
94	19.0
95	10.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2635520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.937604573656316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03749462254876	15.160055005558956
2	9.34847131529604	3.7277224890508243
3	3.270038885413761	1.955902267135782
4	1.6233020300634733	1.2945901591607636
5	0.957851236757052	0.9548629599424879
6	0.6561369897725985	0.7849079908941148
7	0.4568161596206081	0.6375473967360366
8	0.3887121170456669	0.6199990786116253
9	0.3097896706042402	0.5558817558159523
>10	3.845340423639891	19.323771878822875
>50	2.3227264367193112	34.10113807292308
>100	0.7773184233857158	19.39942096947792
>500	0.004452866130817392	0.6001107129813784
>1k	0.0013552201267705106	0.43965144535009326
>5k	0.0	0.0
>10k+	1.936028752529301E-4	0.4444378175382769
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11514	0.43687773190869356	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2709	0.10278806459446334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7943176299174355E-5	0.0	0.0	0.0948579407479359	0.0
2	3.7943176299174355E-5	0.0	0.0	0.3058978873239437	0.0
3	3.7943176299174355E-5	0.0	0.0	0.4648797960174842	0.0
4	3.7943176299174355E-5	0.0	0.0	0.7535135381253035	0.0
5	3.7943176299174355E-5	0.0	0.0	1.360983790675085	0.0
6	3.7943176299174355E-5	0.0	0.0	2.0287457503642545	0.0
7	3.7943176299174355E-5	0.0	0.0	2.3867016755706656	0.0
8	3.7943176299174355E-5	0.0	0.0	3.224449065080136	0.0
9	3.7943176299174355E-5	0.0	0.0	3.5556171078193297	0.0
10	3.7943176299174355E-5	0.0	0.0	4.136451250607091	0.0
11	3.7943176299174355E-5	0.0	0.0	4.839386534725595	0.0
12	3.7943176299174355E-5	0.0	0.0	5.379773251578436	0.0
13	3.7943176299174355E-5	0.0	0.0	5.599919560466246	0.0
14	3.7943176299174355E-5	0.0	0.0	5.6949672170956775	0.0
15	3.7943176299174355E-5	0.0	0.0	5.82905840213696	0.0
16	3.7943176299174355E-5	0.0	0.0	6.122472984458475	0.0
17	3.7943176299174355E-5	0.0	0.0	6.485968613404565	0.0
18	3.7943176299174355E-5	0.0	0.0	6.918369050509956	0.0
19	3.7943176299174355E-5	0.0	0.0	7.166100048567266	0.0
20	3.7943176299174355E-5	0.0	0.0	7.4137172170956775	0.0
21	3.7943176299174355E-5	0.0	0.0	7.728379978144731	0.0
22	7.588635259834871E-5	0.0	0.0	8.088043346284604	0.0
23	7.588635259834871E-5	0.0	0.0	8.454194997571637	0.0
24	7.588635259834871E-5	0.0	0.0	8.72973834385624	0.0
25	7.588635259834871E-5	0.0	0.0	8.96187469645459	0.0
26	7.588635259834871E-5	0.0	0.0	9.180389448761535	0.0
27	7.588635259834871E-5	0.0	0.0	9.420417982030111	0.0
28	7.588635259834871E-5	0.0	0.0	9.659649708596406	0.0
29	7.588635259834871E-5	0.0	0.0	9.93655900922778	0.0
30	7.588635259834871E-5	0.0	0.0	10.258620689655173	0.0
31	1.1382952889752307E-4	0.0	0.0	10.54361188683827	0.0
32	1.1382952889752307E-4	0.0	0.0	10.798779747450219	0.0
33	1.1382952889752307E-4	0.0	0.0	11.054364983001458	0.0
34	1.1382952889752307E-4	0.0	0.0	11.329111522583778	0.0
35	1.1382952889752307E-4	0.0	0.0	11.645785271976688	0.0
36	1.1382952889752307E-4	0.0	0.0	11.919165857212239	0.0
37	1.1382952889752307E-4	0.0	0.0	12.195354237493929	0.0
38	1.1382952889752307E-4	0.0	0.0	12.48300145701797	0.0
39	1.1382952889752307E-4	0.0	0.0	12.870097741622146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	20	7.0349494E-4	45.0	37
CGTTAGG	335	0.0	42.313435	2
CGTAAGG	370	0.0	41.959457	2
CGACGGT	345	0.0	41.739132	28
CGTTTTT	5925	0.0	41.392403	1
CGGTCTA	360	0.0	40.0	31
CTATGCG	125	0.0	39.6	1
TAGGGCG	725	0.0	39.413795	5
CTACCGT	40	3.4605E-7	39.375	24
TAAGGGA	3560	0.0	38.99579	4
TACGGGA	1205	0.0	38.65145	4
TAGGGAC	3290	0.0	38.571426	5
TAGTCGG	70	0.0	38.571426	2
GGGCGAT	3350	0.0	38.417908	7
TTGCGAG	205	0.0	38.414635	1
TATAGCG	130	0.0	38.076927	1
GATCGAC	30	1.1403223E-4	37.500004	22
TCGCTAA	30	1.1403223E-4	37.500004	22
AGGGCGA	1815	0.0	37.066116	6
CGGGTAT	170	0.0	37.058823	6
>>END_MODULE