FastQCFastQC Report
Thu 26 May 2016
SRR1547866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547866_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1053993
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395553.752871224002437No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.3443096870662329No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15740.14933685517835507No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT12670.12020952700824389No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11410.10825498841073897No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11120.10550354698750371No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10810.10256235098335567No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT10630.10085455975514068No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG207.0330186E-445.0000041
TAGCACG302.1653286E-645.0000041
GCGATCG302.1653286E-645.0000049
TTACGAG406.8139343E-945.0000041
GTCGCAA253.8905848E-545.022
CTCCGAT253.8905848E-545.016
TACGGGT453.8562575E-1045.04
TCGATAG253.8905848E-545.01
AGGCCCG253.8905848E-545.038
TATAGCG453.8562575E-1045.01
TAAGCGC253.8905848E-545.018
CGTTTTT99450.044.1176451
TCACGAC2250.044.025
TCGGTAG1650.043.63636428
CGGTCTA2250.043.031
ATCGGTA1700.042.3529427
TCTAGCG800.042.1875041
CTCACGA2350.042.1276624
CGACGGT2300.042.0652228
CGCCAGT700.041.78571326