##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547866_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1053993
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49625566773214	31.0	31.0	33.0	30.0	34.0
2	31.844249439986793	31.0	31.0	34.0	30.0	34.0
3	31.52909174918619	31.0	31.0	34.0	30.0	34.0
4	35.529154368197894	37.0	35.0	37.0	33.0	37.0
5	35.55951889623555	37.0	35.0	37.0	33.0	37.0
6	35.68017434650894	37.0	35.0	37.0	33.0	37.0
7	36.02998881396746	37.0	35.0	37.0	35.0	37.0
8	36.07575287501909	37.0	35.0	37.0	35.0	37.0
9	37.84779215801243	39.0	38.0	39.0	35.0	39.0
10	37.45893948062274	39.0	37.0	39.0	35.0	39.0
11	37.164507733922335	39.0	37.0	39.0	34.0	39.0
12	36.65704231432277	39.0	35.0	39.0	33.0	39.0
13	36.50091224514774	39.0	35.0	39.0	33.0	39.0
14	37.570743828469446	40.0	35.0	41.0	33.0	41.0
15	37.76561988552106	40.0	35.0	41.0	33.0	41.0
16	37.86403325259276	40.0	35.0	41.0	33.0	41.0
17	37.80285258061486	40.0	35.0	41.0	33.0	41.0
18	37.61877830308171	39.0	36.0	41.0	33.0	41.0
19	37.408016941288984	39.0	36.0	41.0	33.0	41.0
20	37.121066268941064	38.0	35.0	41.0	33.0	41.0
21	37.005714459204185	38.0	35.0	41.0	32.0	41.0
22	36.927537469413934	38.0	35.0	41.0	32.0	41.0
23	36.83532053818194	38.0	35.0	41.0	32.0	41.0
24	36.78417503721562	38.0	35.0	41.0	32.0	41.0
25	36.69562321571396	38.0	35.0	41.0	32.0	41.0
26	36.55441734432771	38.0	35.0	40.0	32.0	41.0
27	36.446625357094405	38.0	35.0	40.0	32.0	41.0
28	36.339663546152586	37.0	35.0	40.0	31.0	41.0
29	36.2639543146871	37.0	35.0	40.0	31.0	41.0
30	36.119962846053056	37.0	35.0	40.0	31.0	41.0
31	35.88819090828876	37.0	35.0	40.0	31.0	41.0
32	35.60029715567371	37.0	35.0	40.0	30.0	41.0
33	35.34410854721047	37.0	34.0	40.0	29.0	41.0
34	35.040303872985874	37.0	34.0	40.0	26.0	41.0
35	34.794854425029385	36.0	34.0	40.0	25.0	41.0
36	34.568156524758706	36.0	34.0	40.0	23.0	41.0
37	34.42734249658204	36.0	34.0	40.0	23.0	41.0
38	34.26214974862262	36.0	33.0	40.0	23.0	41.0
39	34.1423823497879	36.0	33.0	40.0	22.0	41.0
40	33.97493436863433	36.0	33.0	40.0	21.0	41.0
41	33.86738526726458	35.0	33.0	40.0	21.0	41.0
42	33.783889456571345	35.0	33.0	40.0	21.0	41.0
43	33.72298203118996	35.0	33.0	40.0	21.0	41.0
44	33.447502972031124	35.0	33.0	40.0	18.0	41.0
45	33.34627174943287	35.0	33.0	40.0	20.0	41.0
46	33.32894430987682	35.0	33.0	40.0	20.0	41.0
47	33.24957091745391	35.0	33.0	40.0	20.0	41.0
48	33.11725694572924	35.0	33.0	40.0	19.0	41.0
49	32.9756782065915	35.0	33.0	39.0	18.0	41.0
50	32.88927630449159	35.0	32.0	39.0	18.0	41.0
51	32.6135790275647	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	6.0
13	8.0
14	15.0
15	46.0
16	73.0
17	200.0
18	453.0
19	887.0
20	1534.0
21	2637.0
22	4016.0
23	6191.0
24	10031.0
25	15834.0
26	21456.0
27	23920.0
28	23624.0
29	24296.0
30	26624.0
31	31080.0
32	38125.0
33	49564.0
34	84881.0
35	135655.0
36	98780.0
37	98393.0
38	132969.0
39	222654.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.81784509005278	23.49550708591044	27.027693732311313	19.658954091725466
2	37.572071161762935	24.58403423931658	26.325981292095868	11.51791330682462
3	27.11203964352704	24.255569059756564	36.51997688789205	12.112414408824348
4	24.793333542063372	25.444191754594197	35.90090256766411	13.86157213567832
5	21.750239327965176	29.466988869945055	35.13116311019143	13.651608691898334
6	19.520338370368684	37.98089740633951	32.15135204882765	10.347412174464157
7	80.76135230499634	3.301065566849116	13.895443328371252	2.0421387997833005
8	81.47416538819517	2.6014404270237086	13.622671118309135	2.3017230664719786
9	76.73665764383635	5.035137804520523	15.092984488511782	3.135220063131349
10	42.2668841254164	28.590417583418485	19.411039731762926	9.731658559402197
11	32.14025140584425	21.4869548469487	30.678002605330395	15.694791141876655
12	29.944601150102514	21.59226863935529	32.8241269154539	15.639003295088298
13	20.966742663376323	29.80427763751752	33.30135968644953	15.927620012656629
14	15.558737107362195	33.86720784673143	34.3300192695777	16.244035776328687
15	14.576662273848118	27.915745170983108	42.378080309831276	15.129512245337493
16	17.814729319834193	25.152728718312172	41.174846512263365	15.857695449590272
17	17.429053134128974	26.745813302365384	31.863494349582965	23.961639213922673
18	19.58608833265496	29.68947611606529	33.71967366007175	17.004761891208005
19	22.12130441094011	31.209505186467084	29.23159831232276	17.437592090270048
20	22.923966288201157	30.188720418446803	29.318126401218986	17.56918689213306
21	20.365505273754188	30.478191031629244	32.0794350626617	17.076868631954863
22	20.103643952094558	27.5019853073028	31.789774694898355	20.604596045704287
23	17.330950015797068	31.780002333981344	31.22354702545463	19.665500624766956
24	18.251164855933578	30.45598974566245	34.97006147099649	16.32278392740749
25	18.49414559679239	30.443371066031748	32.781147502877154	18.28133583429871
26	16.242897248843207	34.2514608730798	29.513668496849597	19.99197338122739
27	16.737682318573274	33.486560157420406	32.57403037781086	17.201727146195466
28	15.554942015743938	31.237019600699433	36.052136968651595	17.155901414905035
29	16.24365626716686	30.86045163487803	34.27413654549888	18.621755552456232
30	17.96767151204989	32.542436240088875	32.84737185161571	16.642520396245516
31	23.471123622263146	31.30760830479899	28.2111930534643	17.010075019473565
32	23.515336439615822	32.220043207118074	27.74354288880476	16.52107746446134
33	21.443690802500587	31.97962415310159	27.80151291327362	18.77517213112421
34	18.435131922128516	31.73227905688178	30.582366296550354	19.250222724439347
35	19.156009575016153	31.30969560518903	30.178094161915688	19.356200657879132
36	24.31818807145778	31.18730390050029	28.511764309630138	15.982743718411793
37	19.518440824559555	33.05382483564882	30.268986606172906	17.158747733618725
38	18.890258284447807	33.858574013299894	28.781974832849933	18.46919286940236
39	18.704109040572376	32.33968347038358	29.788148498139932	19.168058990904115
40	20.92082205479543	30.454566586305603	29.556363277554976	19.068248081343995
41	17.174497363834483	29.584826464691893	31.264249383060417	21.976426788413207
42	18.745475539211363	32.130763676798615	28.669829875530482	20.453930908459544
43	19.356105780588674	31.495275585321725	28.936529939003393	20.212088695086212
44	19.19699656449331	33.11672847922139	29.16338154048461	18.522893415800674
45	18.37640287933601	35.38979860397555	25.717058841946766	20.516739674741675
46	20.637044079040372	33.252213249993126	27.438322645406565	18.67242002555994
47	19.531059504190257	31.672221732022887	29.300763857065466	19.49595490672139
48	19.627549708584404	30.850204887508742	30.248872620596153	19.273372783310705
49	19.273657415182075	29.79839524550922	30.03900405410662	20.88894328520208
50	17.683229395261638	31.839869904259327	30.955423802624875	19.52147689785416
51	16.721553179195688	32.80202050677756	29.663005352027955	20.813420961998798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	1237.0
2	1907.0
3	24684.0
4	47461.0
5	30411.0
6	13361.0
7	12146.0
8	10931.0
9	13433.5
10	15936.0
11	18138.0
12	20340.0
13	20945.0
14	21550.0
15	20208.5
16	18867.0
17	16691.5
18	14516.0
19	13056.5
20	11597.0
21	10569.5
22	9542.0
23	9110.5
24	8679.0
25	8906.5
26	9907.5
27	10681.0
28	12050.0
29	13419.0
30	15396.5
31	17374.0
32	19463.0
33	21552.0
34	23583.0
35	25614.0
36	27733.0
37	29852.0
38	33212.0
39	36572.0
40	42513.5
41	48455.0
42	57269.0
43	66083.0
44	70030.0
45	73977.0
46	78514.0
47	83051.0
48	84037.5
49	85024.0
50	78333.5
51	71643.0
52	64049.5
53	56456.0
54	50822.5
55	45189.0
56	41757.5
57	38326.0
58	37042.0
59	35758.0
60	31857.5
61	27957.0
62	24783.5
63	21610.0
64	18690.0
65	15770.0
66	12799.5
67	9829.0
68	7692.0
69	5555.0
70	4733.5
71	3912.0
72	3303.5
73	2695.0
74	2108.5
75	1174.0
76	826.0
77	643.5
78	461.0
79	348.5
80	236.0
81	159.0
82	82.0
83	73.5
84	65.0
85	43.0
86	21.0
87	16.5
88	12.0
89	10.5
90	9.0
91	10.0
92	11.0
93	7.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1053993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.23710992036838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92194757313652	23.403451117901426
2	8.912884950444148	5.568255338092418
3	3.6307957209879755	3.402466951047136
4	1.98885744636381	2.4850463467203796
5	1.2601673701482135	1.9681993329690641
6	0.9343559300514057	1.7511947335058273
7	0.709529119118288	1.5514547359920074
8	0.6215306384102357	1.5531856696717812
9	0.5036405183625757	1.4159046809198776
>10	6.020910524090387	40.93629673025797
>50	0.4585262447057671	8.720259265409643
>100	0.03132586955367518	1.7944506608096185
>500	0.0030711636816934144	0.6126362961692837
>1k	0.00214981457718539	1.0425177936848742
>5k	0.0	0.0
>10k+	3.0711636816934144E-4	3.7946803468487342
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39555	3.752871224002437	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3629	0.3443096870662329	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1574	0.14933685517835507	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	1267	0.12020952700824389	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1141	0.10825498841073897	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1112	0.10550354698750371	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1081	0.10256235098335567	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	1063	0.10085455975514068	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04962082290869104	0.0
2	0.0	0.0	0.0	0.18472608451858788	0.0
3	0.0	0.0	0.0	0.34079922731934653	0.0
4	0.0	0.0	0.0	0.5121476138835837	0.0
5	0.0	0.0	0.0	1.0154716397547232	0.0
6	0.0	0.0	0.0	1.9559902200488997	0.0
7	0.0	0.0	0.0	2.702864250521588	0.0
8	0.0	0.0	0.0	3.7044838058696783	0.0
9	0.0	0.0	0.0	4.485039274454384	0.0
10	0.0	0.0	0.0	5.232103059507986	0.0
11	0.0	0.0	0.0	5.873853052154995	0.0
12	0.0	0.0	0.0	6.304880582698367	0.0
13	0.0	0.0	0.0	6.528790988175444	0.0
14	0.0	0.0	0.0	6.666078427465837	0.0
15	0.0	0.0	0.0	6.777654121042549	0.0
16	9.487729045638824E-5	0.0	0.0	6.960292905171097	0.0
17	9.487729045638824E-5	0.0	0.0	7.171964140179299	0.0
18	9.487729045638824E-5	0.0	0.0	7.438189817199924	0.0
19	9.487729045638824E-5	0.0	0.0	7.580221121013138	0.0
20	9.487729045638824E-5	0.0	0.0	7.738476441494393	0.0
21	9.487729045638824E-5	0.0	0.0	7.905650227278549	0.0
22	9.487729045638824E-5	0.0	0.0	8.085537569983861	0.0
23	9.487729045638824E-5	0.0	0.0	8.272256077602034	0.0
24	9.487729045638824E-5	0.0	0.0	8.430416520792832	0.0
25	9.487729045638824E-5	0.0	0.0	8.560113776846716	0.0
26	9.487729045638824E-5	0.0	0.0	8.69000078748151	0.0
27	9.487729045638824E-5	0.0	0.0	8.83108331839016	0.0
28	9.487729045638824E-5	0.0	0.0	8.958029133020808	0.0
29	9.487729045638824E-5	0.0	0.0	9.104614546775927	0.0
30	9.487729045638824E-5	0.0	0.0	9.300441274277913	0.0
31	9.487729045638824E-5	0.0	0.0	9.456134907916846	0.0
32	9.487729045638824E-5	0.0	0.0	9.604807622062006	0.0
33	9.487729045638824E-5	0.0	0.0	9.761829537767328	0.0
34	9.487729045638824E-5	0.0	0.0	9.92017973553904	0.0
35	9.487729045638824E-5	0.0	0.0	10.116196217621939	0.0
36	9.487729045638824E-5	0.0	0.0	10.280998071144685	0.0
37	9.487729045638824E-5	0.0	0.0	10.448551366090667	0.0
38	9.487729045638824E-5	0.0	0.0	10.654814595542854	0.0
39	9.487729045638824E-5	0.0	0.0	10.997036982219047	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	20	7.0330186E-4	45.000004	1
TAGCACG	30	2.1653286E-6	45.000004	1
GCGATCG	30	2.1653286E-6	45.000004	9
TTACGAG	40	6.8139343E-9	45.000004	1
GTCGCAA	25	3.8905848E-5	45.0	22
CTCCGAT	25	3.8905848E-5	45.0	16
TACGGGT	45	3.8562575E-10	45.0	4
TCGATAG	25	3.8905848E-5	45.0	1
AGGCCCG	25	3.8905848E-5	45.0	38
TATAGCG	45	3.8562575E-10	45.0	1
TAAGCGC	25	3.8905848E-5	45.0	18
CGTTTTT	9945	0.0	44.117645	1
TCACGAC	225	0.0	44.0	25
TCGGTAG	165	0.0	43.636364	28
CGGTCTA	225	0.0	43.0	31
ATCGGTA	170	0.0	42.35294	27
TCTAGCG	80	0.0	42.187504	1
CTCACGA	235	0.0	42.12766	24
CGACGGT	230	0.0	42.06522	28
CGCCAGT	70	0.0	41.785713	26
>>END_MODULE