FastQCFastQC Report
Thu 26 May 2016
SRR1547865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547865_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1540069
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58260.3782947387422252No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41660.2705073603845023No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15440.10025524830380977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG302.1659853E-645.00000411
ACGGGTC302.1659853E-645.0000045
ATATCCG302.1659853E-645.0000041
AATCGAC302.1659853E-645.00000429
CGGACGT302.1659853E-645.00000433
ATCTCGA207.034035E-445.044
GATCGAC207.034035E-445.041
CGCATCG502.1827873E-1145.021
CCCGTAT253.8914266E-545.034
CATAGTA406.8175723E-945.040
CTCGCTA207.034035E-445.028
GCGATAC1000.045.09
TCCGTAA207.034035E-445.023
TTACGAA406.8175723E-945.031
TGTACGA207.034035E-445.015
CCCGGTA207.034035E-445.017
CGTCCAC207.034035E-445.023
TATCGTG253.8914266E-545.01
CGTCAAA207.034035E-445.027
TAATGCG800.045.01