##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547865_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1540069
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51219328484633	31.0	31.0	33.0	30.0	34.0
2	31.926296808779348	31.0	31.0	34.0	30.0	34.0
3	32.01491686411453	33.0	31.0	34.0	30.0	34.0
4	35.76954668914185	37.0	35.0	37.0	35.0	37.0
5	35.66879276188275	37.0	35.0	37.0	33.0	37.0
6	35.70857020042609	37.0	35.0	37.0	33.0	37.0
7	36.02361842229147	37.0	35.0	37.0	35.0	37.0
8	36.047051787939374	37.0	35.0	37.0	35.0	37.0
9	37.820558039931974	39.0	38.0	39.0	35.0	39.0
10	37.35877418479302	39.0	37.0	39.0	35.0	39.0
11	37.02443981406028	39.0	37.0	39.0	33.0	39.0
12	35.969776678837114	37.0	35.0	39.0	32.0	39.0
13	35.50338653657726	37.0	35.0	39.0	31.0	39.0
14	36.42269469744537	38.0	35.0	41.0	31.0	41.0
15	36.760230223451025	38.0	35.0	41.0	32.0	41.0
16	36.95731814613501	38.0	35.0	41.0	33.0	41.0
17	36.913309728330354	38.0	35.0	41.0	32.0	41.0
18	36.85858101163	38.0	35.0	41.0	32.0	41.0
19	36.770797931780976	38.0	35.0	40.0	32.0	41.0
20	36.61678080657425	38.0	35.0	40.0	32.0	41.0
21	36.457091857572614	37.0	35.0	40.0	31.0	41.0
22	36.33897377325302	37.0	35.0	40.0	31.0	41.0
23	36.32419975988089	37.0	35.0	40.0	31.0	41.0
24	36.32245438353736	37.0	35.0	40.0	31.0	41.0
25	36.31026142335181	37.0	35.0	40.0	31.0	41.0
26	36.192430339160126	37.0	35.0	40.0	31.0	41.0
27	36.09689825585737	37.0	35.0	40.0	31.0	41.0
28	36.08403324786097	37.0	35.0	40.0	31.0	41.0
29	36.1360581896006	37.0	35.0	40.0	31.0	41.0
30	36.08505398134759	36.0	35.0	40.0	31.0	41.0
31	35.89457030821346	36.0	35.0	40.0	31.0	41.0
32	35.70506126673545	36.0	34.0	40.0	30.0	41.0
33	35.613912753259754	36.0	34.0	40.0	30.0	41.0
34	35.56406433737709	36.0	35.0	40.0	30.0	41.0
35	35.490948782164956	36.0	34.0	40.0	30.0	41.0
36	35.33771863468455	36.0	34.0	40.0	29.0	41.0
37	35.21782011065738	36.0	34.0	40.0	29.0	41.0
38	35.152253567859624	36.0	34.0	40.0	29.0	41.0
39	35.128749426162074	36.0	34.0	40.0	29.0	41.0
40	34.98612789426967	36.0	34.0	40.0	27.0	41.0
41	34.97221358263818	36.0	34.0	40.0	28.0	41.0
42	34.8762873611507	36.0	34.0	40.0	27.0	41.0
43	34.77665935747035	35.0	34.0	40.0	27.0	41.0
44	34.62294676407356	35.0	34.0	40.0	27.0	41.0
45	34.47778119032329	35.0	34.0	40.0	26.0	41.0
46	34.43980432045577	35.0	34.0	40.0	26.0	41.0
47	34.37263070680599	35.0	34.0	40.0	26.0	41.0
48	34.31693060505731	35.0	34.0	40.0	26.0	41.0
49	34.23975029690228	35.0	34.0	39.0	25.0	41.0
50	34.13466994011307	35.0	34.0	39.0	25.0	41.0
51	33.76899022056804	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	2.0
13	5.0
14	9.0
15	36.0
16	80.0
17	235.0
18	494.0
19	1050.0
20	2012.0
21	3200.0
22	5043.0
23	7487.0
24	10896.0
25	15581.0
26	20343.0
27	24058.0
28	27435.0
29	31933.0
30	37885.0
31	46644.0
32	59961.0
33	81030.0
34	161022.0
35	271742.0
36	93563.0
37	120343.0
38	184908.0
39	333011.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9022271080062	24.014703237322482	30.211633374868267	12.871436279803047
2	29.770029784379794	26.309080956762326	30.412273735787167	13.508615523070718
3	28.80838455939312	25.459248903782882	31.187693538406396	14.544672998417605
4	26.216357838512433	27.53318195483449	30.60453784862886	15.645922358024219
5	23.542062076439432	32.21089444693712	29.20362659075665	15.043416885866803
6	21.785387537831095	40.01814204428503	27.048723141625473	11.147747276258402
7	88.8122545158691	3.563736429991124	5.863763246971402	1.7602458071683802
8	89.19295174436989	2.7666292873890717	5.3554094004879005	2.685009567753133
9	84.90009213872884	4.635766319561007	7.609269454810141	2.8548720869000026
10	53.89219573928181	24.885962901662197	11.64902351777745	9.57281784127854
11	47.10340900310311	17.826538940787717	21.042433813030456	14.027618243078718
12	43.40876934734742	19.026420244807213	23.073901234295345	14.490909173550016
13	22.06310236749133	40.73187629904894	23.131106463411705	14.073914870048029
14	14.661875539342716	42.63464818784094	26.68718089903764	16.016295373778707
15	12.991755564198748	24.80155109933386	48.20011311181512	14.006580224652271
16	15.984933142605948	19.743660835975533	45.903852359861794	18.367553661556723
17	16.16486014587658	20.103384978205522	27.453185539089482	36.27856933682841
18	21.978300972229167	23.487389201392926	33.304871405112365	21.229438421265538
19	27.510780361139663	26.3857009004142	24.665972758363424	21.43754598008271
20	30.376431185875436	24.716684771916064	24.926870159713623	19.980013882494877
21	21.82213913792174	28.730076379694676	27.557076988108975	21.890707494274608
22	22.00310505568257	24.431178083579372	26.737113726722633	26.82860313401542
23	18.581310317914326	30.6339521151325	24.71350309628984	26.071234470663327
24	20.272922836574207	24.76720198900179	36.88185399485347	18.078021179570523
25	17.005471832755546	25.868581212919683	34.01354095173657	23.112406002588195
26	16.72600383489311	35.24166774345825	26.050715909481976	21.981612512166663
27	17.641742025844295	33.42863209375684	29.19576979992455	19.733856080474315
28	14.182351569962126	28.619237190022005	39.27973357037899	17.918677669636878
29	14.290918134187494	24.539355054870917	38.8024822264457	22.36724458449589
30	18.4925480611583	29.323036824973425	32.82612662159942	19.35828849226885
31	29.322452435572693	26.230577980596976	22.97747698317413	21.469492600656203
32	29.085904592586438	27.49948216605879	24.567795339040003	18.846817902314765
33	25.224129568220643	28.13075258316348	24.78427914593437	21.860838702681505
34	17.32299007382137	27.1711851871572	29.28979156128719	26.216033177734243
35	18.443784012274776	26.509136928280487	30.315265095265215	24.731813964179526
36	28.160361646134035	22.707878672968548	28.61248424583574	20.519275435061676
37	19.15284315183281	29.232001942770097	30.97166425660149	20.643490648795606
38	18.57481710235061	31.291455123114613	25.165820492458458	24.967907282076325
39	18.347944150554294	30.160466836226167	29.61263423911526	21.87895477410428
40	22.80670541384834	25.708848110052212	26.182917778359283	25.301528697740167
41	16.043242218368135	22.422112256009306	29.27641553722593	32.25822998839662
42	21.766362416229406	24.09060892726235	25.652097406025316	28.49093125048293
43	21.89473328792411	25.689108734738507	26.308171906583404	26.107986070753974
44	19.228748841772674	30.140792393068104	27.528701636095526	23.1017571290637
45	16.955279276448003	35.49087735679375	22.74378615503591	24.81005721172233
46	22.083231335738855	30.880499510086885	26.25784948596459	20.778419668209672
47	19.99903900409657	26.087272713105712	28.14854399380807	25.76514428898965
48	21.7494151236081	22.433735111868362	31.78584855613612	24.03100120838742
49	19.805606112453404	21.821554748521006	32.29978656800442	26.073052571021165
50	18.142109217184423	29.34667212962536	29.412253606818915	23.0989650463713
51	16.65392914213584	31.762018455017277	25.11958879764478	26.464463605202106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	705.0
1	1141.0
2	1577.0
3	5214.0
4	8851.0
5	6671.5
6	4492.0
7	4527.5
8	4563.0
9	4869.5
10	5176.0
11	5281.0
12	5386.0
13	5223.0
14	5060.0
15	4813.0
16	4566.0
17	4293.5
18	4021.0
19	4184.5
20	4348.0
21	4126.5
22	3905.0
23	5085.0
24	6265.0
25	6440.0
26	8794.5
27	10974.0
28	12720.5
29	14467.0
30	18420.5
31	22374.0
32	24018.0
33	25662.0
34	30807.5
35	35953.0
36	37753.0
37	39553.0
38	51871.5
39	64190.0
40	84109.5
41	104029.0
42	129333.5
43	154638.0
44	163869.5
45	173101.0
46	164111.5
47	155122.0
48	147409.5
49	139697.0
50	133436.5
51	127176.0
52	113167.5
53	99159.0
54	86307.0
55	73455.0
56	66745.5
57	60036.0
58	54558.5
59	49081.0
60	44533.5
61	39986.0
62	34590.0
63	29194.0
64	25861.0
65	22528.0
66	18523.0
67	14518.0
68	11540.0
69	8562.0
70	6911.5
71	5261.0
72	3946.5
73	2632.0
74	2162.5
75	1214.0
76	735.0
77	559.5
78	384.0
79	308.5
80	233.0
81	158.5
82	84.0
83	58.5
84	33.0
85	23.5
86	14.0
87	9.0
88	4.0
89	6.5
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1540069.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.197427085586952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00386610105193	14.012722386821933
2	9.525222170435299	3.466690718410253
3	3.464032494377905	1.8910943621563745
4	1.6888815057073947	1.2293319222522647
5	0.9999264495352705	0.9098044328183964
6	0.6198090892384777	0.6767358425040751
7	0.43543543716748007	0.5546663232835128
8	0.34056080905932484	0.49578643928524513
9	0.23510818182227006	0.38505275963421115
>10	2.3222747902296614	10.036755510795386
>50	1.6887617096094327	23.239980795274757
>100	1.6674535370763022	41.290371916379016
>500	0.006139638321485182	0.7160955076509787
>1k	0.0021669311722888876	0.7120208085435668
>5k	3.611551953814813E-4	0.38289027419003324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5826	0.3782947387422252	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4166	0.2705073603845023	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1544	0.10025524830380977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09765796207832246	0.0
2	0.0	0.0	0.0	0.37842460305349956	0.0
3	0.0	0.0	0.0	0.5517934586047768	0.0
4	0.0	0.0	0.0	0.9882674087979175	0.0
5	0.0	0.0	0.0	1.6242778732641199	0.0
6	0.0	0.0	0.0	2.285352149806275	0.0
7	0.0	0.0	0.0	2.640790769764212	0.0
8	0.0	0.0	0.0	3.584060194705562	0.0
9	0.0	0.0	0.0	3.857359637782463	0.0
10	0.0	0.0	0.0	4.387595620715695	0.0
11	6.493215563718248E-5	0.0	0.0	5.065941850657341	0.0
12	6.493215563718248E-5	0.0	0.0	5.665590307966721	0.0
13	6.493215563718248E-5	0.0	0.0	5.885060994020398	0.0
14	6.493215563718248E-5	0.0	0.0	5.9572006189333075	0.0
15	6.493215563718248E-5	0.0	0.0	6.096869685708887	0.0
16	6.493215563718248E-5	0.0	0.0	6.4149073840198065	0.0
17	6.493215563718248E-5	0.0	0.0	6.773397815292691	0.0
18	6.493215563718248E-5	0.0	0.0	7.336554401133975	0.0
19	6.493215563718248E-5	0.0	0.0	7.5711542794511155	0.0
20	1.2986431127436497E-4	0.0	0.0	7.830882901999845	0.0
21	1.2986431127436497E-4	0.0	0.0	8.144505213727436	0.0
22	1.2986431127436497E-4	0.0	0.0	8.45416666396116	0.0
23	1.2986431127436497E-4	0.0	0.0	8.785710250644614	0.0
24	1.2986431127436497E-4	0.0	0.0	9.028361716260765	0.0
25	1.2986431127436497E-4	0.0	0.0	9.22088555772501	0.0
26	1.2986431127436497E-4	0.0	0.0	9.422824561756649	0.0
27	1.2986431127436497E-4	0.0	0.0	9.615348403220894	0.0
28	1.2986431127436497E-4	0.0	0.0	9.826702569819924	0.0
29	1.2986431127436497E-4	0.0	0.0	10.05656240077555	0.0
30	1.2986431127436497E-4	0.0	0.0	10.384145125965135	0.0
31	1.2986431127436497E-4	0.0	0.0	10.653613571859443	0.0
32	1.2986431127436497E-4	0.0	0.0	10.88412272437144	0.0
33	1.2986431127436497E-4	0.0	0.0	11.106190696650605	0.0
34	1.9479646691154747E-4	0.0	0.0	11.38169783301917	0.0
35	1.9479646691154747E-4	0.0	0.0	11.723500700293298	0.0
36	1.9479646691154747E-4	0.0	0.0	11.981476154639825	0.0
37	1.9479646691154747E-4	0.0	0.0	12.225036670434896	0.0
38	1.9479646691154747E-4	0.0	0.0	12.485414614539998	0.0
39	1.9479646691154747E-4	0.0	0.0	12.822217705830063	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	30	2.1659853E-6	45.000004	11
ACGGGTC	30	2.1659853E-6	45.000004	5
ATATCCG	30	2.1659853E-6	45.000004	1
AATCGAC	30	2.1659853E-6	45.000004	29
CGGACGT	30	2.1659853E-6	45.000004	33
ATCTCGA	20	7.034035E-4	45.0	44
GATCGAC	20	7.034035E-4	45.0	41
CGCATCG	50	2.1827873E-11	45.0	21
CCCGTAT	25	3.8914266E-5	45.0	34
CATAGTA	40	6.8175723E-9	45.0	40
CTCGCTA	20	7.034035E-4	45.0	28
GCGATAC	100	0.0	45.0	9
TCCGTAA	20	7.034035E-4	45.0	23
TTACGAA	40	6.8175723E-9	45.0	31
TGTACGA	20	7.034035E-4	45.0	15
CCCGGTA	20	7.034035E-4	45.0	17
CGTCCAC	20	7.034035E-4	45.0	23
TATCGTG	25	3.8914266E-5	45.0	1
CGTCAAA	20	7.034035E-4	45.0	27
TAATGCG	80	0.0	45.0	1
>>END_MODULE