FastQCFastQC Report
Thu 26 May 2016
SRR1547864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547864_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2234832
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82390.3686630583417456No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55380.24780386176679053No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30680.13728101262197784No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23000.10291601337371221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA207.034717E-445.00000424
GTCGCAA406.8193913E-945.00000427
TAAGTCG207.034717E-445.0000041
CGCTATA207.034717E-445.00000420
TCGCTAG207.034717E-445.00000433
GCGATAT1500.045.0000049
TTACGAA406.8193913E-945.00000432
CCGAAAC207.034717E-445.00000423
CACGTAT207.034717E-445.00000414
CGACTCG207.034717E-445.00000422
ATCGATA207.034717E-445.00000429
GTCGGTT207.034717E-445.00000442
GTTGCGA207.034717E-445.00000413
TACCCGC253.8919923E-545.021
CGTGCAC253.8919923E-545.045
CGTGTAA253.8919923E-545.041
CAATACG253.8919923E-545.01
TAGTACG502.1827873E-1145.01
CGACCGT302.1664255E-644.99999625
TACGCCT302.1664255E-644.99999624