##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547864_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2234832
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.589753502724143	31.0	31.0	33.0	30.0	34.0
2	32.00787307502309	31.0	31.0	34.0	30.0	34.0
3	32.09414577919056	33.0	31.0	34.0	30.0	34.0
4	35.83716494125733	37.0	35.0	37.0	35.0	37.0
5	35.75903602597421	37.0	35.0	37.0	35.0	37.0
6	35.801645940276494	37.0	35.0	37.0	35.0	37.0
7	36.07582851865375	37.0	35.0	37.0	35.0	37.0
8	36.09271658898745	37.0	35.0	37.0	35.0	37.0
9	37.840361154663974	39.0	38.0	39.0	35.0	39.0
10	37.45942692784066	39.0	37.0	39.0	35.0	39.0
11	37.153402582386505	39.0	37.0	39.0	34.0	39.0
12	36.278919847218944	38.0	35.0	39.0	33.0	39.0
13	35.95649113669394	38.0	35.0	39.0	32.0	39.0
14	36.99340934799573	39.0	35.0	41.0	32.0	41.0
15	37.224929211681236	39.0	35.0	41.0	33.0	41.0
16	37.3751776419883	39.0	35.0	41.0	33.0	41.0
17	37.29200942173729	39.0	35.0	41.0	33.0	41.0
18	37.23830068658405	38.0	35.0	41.0	33.0	41.0
19	37.13866545673232	38.0	35.0	41.0	32.0	41.0
20	37.00773033498715	38.0	35.0	41.0	32.0	41.0
21	36.88214281878906	38.0	35.0	41.0	32.0	41.0
22	36.75996942946942	38.0	35.0	41.0	32.0	41.0
23	36.71197700766769	38.0	35.0	41.0	32.0	41.0
24	36.73009380570888	38.0	35.0	41.0	32.0	41.0
25	36.698404175347406	38.0	35.0	41.0	32.0	41.0
26	36.57302875562906	38.0	35.0	40.0	32.0	41.0
27	36.49526765322852	38.0	35.0	40.0	31.0	41.0
28	36.46942320496574	38.0	35.0	40.0	31.0	41.0
29	36.54328781760777	38.0	35.0	40.0	32.0	41.0
30	36.47721528956092	38.0	35.0	40.0	31.0	41.0
31	36.31481561030091	38.0	35.0	40.0	31.0	41.0
32	36.12447736563643	38.0	35.0	40.0	31.0	41.0
33	36.040397220014746	38.0	35.0	40.0	30.0	41.0
34	35.94093157785462	37.0	35.0	40.0	30.0	41.0
35	35.83018589316781	37.0	35.0	40.0	30.0	41.0
36	35.66236343492486	37.0	35.0	40.0	30.0	41.0
37	35.551049027398925	37.0	35.0	40.0	30.0	41.0
38	35.444641028945355	37.0	34.0	40.0	29.0	41.0
39	35.37083145399744	37.0	35.0	40.0	29.0	41.0
40	35.15388718257122	37.0	34.0	40.0	27.0	41.0
41	35.1024815288129	36.0	34.0	40.0	27.0	41.0
42	35.04527901873608	36.0	34.0	40.0	27.0	41.0
43	34.95905061320045	36.0	34.0	40.0	27.0	41.0
44	34.77601537833716	36.0	34.0	40.0	27.0	41.0
45	34.63638609076656	36.0	34.0	40.0	26.0	41.0
46	34.57547278721622	35.0	34.0	40.0	26.0	41.0
47	34.501821165975784	35.0	34.0	40.0	26.0	41.0
48	34.42112606227224	35.0	34.0	40.0	25.0	41.0
49	34.2960576007503	36.0	34.0	40.0	24.0	41.0
50	34.203880649641675	35.0	34.0	40.0	24.0	41.0
51	33.850136833551694	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	7.0
12	2.0
13	5.0
14	20.0
15	47.0
16	117.0
17	280.0
18	648.0
19	1317.0
20	2342.0
21	3777.0
22	6204.0
23	9603.0
24	14450.0
25	20902.0
26	27695.0
27	33107.0
28	37338.0
29	43033.0
30	52024.0
31	63139.0
32	79399.0
33	107566.0
34	211089.0
35	362539.0
36	143505.0
37	181863.0
38	293119.0
39	539575.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.48518725344903	23.862867544406022	28.74019165646456	12.91175354568039
2	29.716148685896748	26.836066424679792	29.811144640849964	13.636640248573496
3	29.957061649376776	25.64935529829535	30.792113232672524	13.601469819655348
4	26.926811500819746	28.193752371542917	29.859783643692232	15.019652483945102
5	24.25819927403939	32.750291744524866	28.2378720190153	14.75363696242044
6	21.856363252360804	41.730743071515	25.17504671492085	11.237846961203347
7	87.61213370848458	4.507855624046908	5.975348482570502	1.9046621848980145
8	88.06849910865783	3.6167819326016453	5.429669881225972	2.885049077514551
9	83.1534540403932	6.336225720770063	7.3116905431817685	3.1986296956549753
10	49.8284434803153	28.595974999463046	11.797486343492485	9.778095176729169
11	44.9630218289339	19.844489429183042	21.579340192014435	13.613148549868626
12	40.86490617639268	21.566140094646936	23.480959642604006	14.087994086356378
13	21.873903720727107	39.21283568518797	23.335355856726594	15.577904737358333
14	15.322941500748154	42.30273237540898	26.789977949125483	15.584348174717382
15	14.360676775703945	26.09923251501679	44.81271970331551	14.72737100596376
16	16.233613980827194	21.98308418708878	43.76445298796509	18.01884884411893
17	16.685146802981162	23.150778224045474	26.0229851729347	34.14108980003866
18	22.04863721299856	26.079409995919157	31.98499931985939	19.886953471222892
19	27.260214638057807	26.722232364669917	25.253441869455962	20.76411112781632
20	30.75439227646642	24.519382217544763	25.027026640033796	19.699198865955026
21	22.36861652240526	29.094849187768922	27.708436249346708	20.828098040479105
22	22.785694853125424	25.440391045053946	26.280006729812357	25.49390737200828
23	20.531431445406188	30.036038503117908	25.12144089578098	24.311089155694926
24	20.885775754061157	25.29071536473435	35.371294128596695	18.4522147526078
25	17.75502588114006	27.438930532585893	32.511079132570146	22.2949644537039
26	17.796639747417256	35.22094725688552	26.663301760490278	20.31911123520694
27	19.324987292109654	33.87990685653329	28.313090200963654	18.482015650393407
28	15.37019337471452	30.27404297056781	36.40510785598202	17.950655798735653
29	16.699286568296856	24.817704418050216	36.59885843768122	21.884150575971706
30	19.338724342590403	29.97742112158766	31.715046142170866	18.968808393651067
31	29.285064828139205	26.962653121129463	23.845371822132492	19.90691022859884
32	28.259394889638234	28.223016316215265	26.068402457097445	17.44918633704905
33	26.624283167593806	28.785250971885134	25.098799372838766	19.491666487682295
34	19.58138240368851	28.60219470635824	28.8328160685009	22.98360682145235
35	20.716680269478868	26.03350050473593	31.246823027413246	22.002996198371957
36	28.341235493316724	25.034409745341037	28.641705506275194	17.98264925506705
37	21.104091940691738	31.004836157706713	29.4988616593999	18.39221024220165
38	20.325688910844306	30.409623631664484	25.539861609284277	23.724825848206933
39	20.898215167851543	29.686750502946087	28.992649111879548	20.422385217322823
40	23.55116626216199	26.726617481761494	27.197480615992614	22.524735640083907
41	17.485788640935873	25.140189508652107	29.551035603571098	27.822986246840927
42	23.3337897434796	25.45041416983469	26.256291300643632	24.959504786042082
43	23.397329195214674	26.33285186537512	26.682318849918023	23.587500089492185
44	20.255840260028492	30.664318391718037	27.149199581892507	21.93064176636096
45	19.50513506160642	34.593920258882996	22.76649877932659	23.134445900183994
46	23.163978321412973	31.059784359629717	26.320725674234126	19.455511644723185
47	22.75692311547356	27.18522018657331	27.495444847755895	22.562411850197243
48	23.755700652219048	23.57098878125962	30.239230510391835	22.4340800561295
49	21.020774715951802	23.255394588944494	31.546219134145208	24.177611560958496
50	19.02662034551143	29.653683140388182	29.08813727385325	22.231559240247144
51	18.511950786457327	31.138582228999763	25.186143745928106	25.163323238614804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1201.0
1	2192.0
2	3183.0
3	8370.5
4	13558.0
5	10319.0
6	7080.0
7	7139.5
8	7199.0
9	7674.0
10	8149.0
11	8625.0
12	9101.0
13	8866.5
14	8632.0
15	8419.0
16	8206.0
17	8001.5
18	7797.0
19	8093.5
20	8390.0
21	9486.5
22	10583.0
23	10295.0
24	10007.0
25	10771.5
26	15432.0
27	19328.0
28	23950.0
29	28572.0
30	34770.0
31	40968.0
32	42646.5
33	44325.0
34	49442.0
35	54559.0
36	57228.0
37	59897.0
38	74536.0
39	89175.0
40	120131.0
41	151087.0
42	184175.5
43	217264.0
44	223154.5
45	229045.0
46	222341.0
47	215637.0
48	209676.5
49	203716.0
50	188532.5
51	173349.0
52	155570.5
53	137792.0
54	121561.0
55	105330.0
56	95593.5
57	85857.0
58	81004.5
59	76152.0
60	69678.0
61	63204.0
62	55434.0
63	47664.0
64	40283.5
65	32903.0
66	25943.0
67	18983.0
68	14308.0
69	9633.0
70	7723.5
71	5814.0
72	5056.5
73	4299.0
74	3224.0
75	1967.0
76	1785.0
77	1356.5
78	928.0
79	759.0
80	590.0
81	347.5
82	105.0
83	73.5
84	42.0
85	34.5
86	27.0
87	20.0
88	13.0
89	11.5
90	10.0
91	6.5
92	3.0
93	4.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2234832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.354250204584517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34777185051297	13.596668356655057
2	9.173774117000292	3.1840794269352894
3	3.261500759612019	1.6980270067424832
4	1.5664840192684413	1.087406224474709
5	0.9161557943200939	0.794959844050539
6	0.5718071934879615	0.5953971062742813
7	0.3942744814842529	0.4789636600672385
8	0.2904555512160913	0.4032510647291649
9	0.23026579803061895	0.35964812453235173
>10	2.1061976157399536	8.549357308535626
>50	1.1973793516949056	15.521298389306299
>100	1.9300743778061218	51.084108370144406
>500	0.0107211826958708	1.1845546938496583
>1k	0.0026149226087489753	0.8370798713845458
>5k	5.22984521749795E-4	0.6252005523183826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8239	0.3686630583417456	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5538	0.24780386176679053	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3068	0.13728101262197784	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2300	0.10291601337371221	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12658669644966603	0.0
2	0.0	0.0	0.0	0.4644644429648403	0.0
3	0.0	0.0	0.0	0.6615709816218848	0.0
4	0.0	0.0	0.0	1.078336089692648	0.0
5	0.0	0.0	0.0	1.7104641422710969	0.0
6	0.0	0.0	0.0	2.3782995768809467	0.0
7	0.0	0.0	0.0	2.7040063861623604	0.0
8	0.0	0.0	0.0	3.4997709000050117	0.0
9	0.0	0.0	0.0	3.7648914996742486	0.0
10	0.0	0.0	0.0	4.2866756874789695	0.0
11	0.0	0.0	0.0	4.936612684980347	0.0
12	0.0	0.0	0.0	5.430833279638022	0.0
13	0.0	0.0	0.0	5.616619056823958	0.0
14	0.0	0.0	0.0	5.6938060668542425	0.0
15	0.0	0.0	0.0	5.823211767148493	0.0
16	0.0	0.0	0.0	6.12059429970575	0.0
17	0.0	0.0	0.0	6.451402163563078	0.0
18	0.0	0.0	0.0	6.9055750051905465	0.0
19	0.0	0.0	0.0	7.113241621741589	0.0
20	0.0	0.0	0.0	7.329052027176987	0.0
21	0.0	0.0	0.0	7.61565075137639	0.0
22	0.0	0.0	0.0	7.898580295968556	0.0
23	0.0	0.0	0.0	8.204822554894507	0.0
24	0.0	0.0	0.0	8.420990929072074	0.0
25	0.0	0.0	0.0	8.593397624519428	0.0
26	0.0	0.0	0.0	8.774306077593305	0.0
27	0.0	0.0	0.0	8.947697187081625	0.0
28	0.0	0.0	0.0	9.126323589424171	0.0
29	0.0	0.0	0.0	9.325622686627003	0.0
30	0.0	0.0	0.0	9.57517164601187	0.0
31	0.0	0.0	0.0	9.805390293319586	0.0
32	0.0	0.0	0.0	9.998917144554937	0.0
33	0.0	0.0	0.0	10.194994523078245	0.0
34	0.0	0.0	0.0	10.40122926466061	0.0
35	0.0	0.0	0.0	10.681474043686505	0.0
36	0.0	0.0	0.0	10.905249253635173	0.0
37	0.0	0.0	0.0	11.11457147561875	0.0
38	0.0	0.0	0.0	11.3157051626252	0.0
39	0.0	0.0	0.0	11.540196310058207	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	20	7.034717E-4	45.000004	24
GTCGCAA	40	6.8193913E-9	45.000004	27
TAAGTCG	20	7.034717E-4	45.000004	1
CGCTATA	20	7.034717E-4	45.000004	20
TCGCTAG	20	7.034717E-4	45.000004	33
GCGATAT	150	0.0	45.000004	9
TTACGAA	40	6.8193913E-9	45.000004	32
CCGAAAC	20	7.034717E-4	45.000004	23
CACGTAT	20	7.034717E-4	45.000004	14
CGACTCG	20	7.034717E-4	45.000004	22
ATCGATA	20	7.034717E-4	45.000004	29
GTCGGTT	20	7.034717E-4	45.000004	42
GTTGCGA	20	7.034717E-4	45.000004	13
TACCCGC	25	3.8919923E-5	45.0	21
CGTGCAC	25	3.8919923E-5	45.0	45
CGTGTAA	25	3.8919923E-5	45.0	41
CAATACG	25	3.8919923E-5	45.0	1
TAGTACG	50	2.1827873E-11	45.0	1
CGACCGT	30	2.1664255E-6	44.999996	25
TACGCCT	30	2.1664255E-6	44.999996	24
>>END_MODULE