FastQCFastQC Report
Thu 26 May 2016
SRR1547863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547863_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1687604
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101600.6020369707585429No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45180.2677168340440056No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT27510.16301217584219996No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC21310.12627369928016288No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC20880.12372570816376353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT600.045.00000429
AATCCGG253.8915845E-545.00000444
CTCCGAT351.2123746E-745.00000445
TACGCTT302.1661072E-645.00000428
GTTCGCC253.8915845E-545.00000427
TACGACG302.1661072E-645.0000041
TTGACGA253.8915845E-545.00000432
GGACGTA253.8915845E-545.0000049
CGCAATC253.8915845E-545.00000418
TAGGACG600.045.0000041
CGCCCTA253.8915845E-545.00000417
CTTACGT302.1661072E-645.00000429
AAGTACG453.8562575E-1045.01
GTTTACG406.8175723E-945.01
TTTCGCG750.045.01
CACGCTA207.034227E-445.019
CATTACG207.034227E-445.030
TACGATA207.034227E-445.037
CGGATAG207.034227E-445.014
CACTACG406.8175723E-945.01